Application of nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants

A technology of nitrogen-doped carbon dots and environmental pollutants, which is applied in the analysis of materials, material analysis by electromagnetic means, and measurement devices, etc., can solve the problems of high toxicity, poor water solubility, and complex synthesis process, so as to avoid decomposition and sensitivity High, simple preparation steps

Inactive Publication Date: 2015-12-30
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these materials still have many deficiencies, such as poor water solubility, complex synthesis process, and high toxicity.

Method used

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  • Application of nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants
  • Application of nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants
  • Application of nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Method for preparing nitrogen-doped carbon dots:

[0035] Step 1, weigh 0.2682g of malic acid in a microwave reactor, add 10mL of secondary water, then add 121μL of ethanolamine, stir well, and ultrasonically obtain a clear solution;

[0036] Step 2, the microwave reactor was placed in a microwave oven (700 watts) and reacted for 10 minutes under high heat to obtain a brown solid;

[0037] Step 3, take out the microwave reactor, cool it naturally, add 20mL of secondary water into it, stir and dissolve to obtain a brown solution, remove insoluble matter by filtration to obtain a clear brown solution, pass through a 500-1000Da dialysis bag, and dialyze in a glass container for at least Impurities were removed in 3 days, and a pure aqueous solution of nitrogen-doped carbon quantum dots was obtained.

Embodiment 2

[0039] Instrument and detection conditions: Bruker Autoflex II mass spectrometer (Bruker Daltonics, Germany); nitrogen laser, wavelength 337nm, negative ion mode; laser energy 60-70%; accumulation times: 500 times.

[0040] Solution configuration: the matrix is ​​made into a nitrogen-doped carbon dot aqueous solution with a concentration of 1.0mg / mL;

[0041] Pipette 1 μL of the matrix solution onto the MALDI target, and after natural drying, perform MALDI-TOFMS analysis ( figure 1 ).

Embodiment 3

[0043] Instrument and detection conditions: Bruker Autoflex II mass spectrometer (Bruker Daltonics, Germany); nitrogen laser, wavelength 337nm, negative ion mode; laser energy 60-70%; accumulation times: 500 times.

[0044] Solution configuration: the matrix is ​​made into a carbon dot aqueous solution with a concentration of 2.0mg / mL; the mixture sample of 1-hydroxypyrene, 2,4-dihydroxyanthraquinone, and 11,12-dihydroxybenzopyrene is mixed with acetonitrile / acetone (1 : 1) is a solvent, and a solution with a concentration of 800 μg / mL is prepared.

[0045] Pipette 1 μL of the matrix solution on the MALDI target, and after natural drying, pipette 1 μL of the hydroxy polycyclic aromatic hydrocarbon mixture solution on the MALDI target, and after natural drying, perform MALDI-TOFMS analysis ( figure 2 ).

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Abstract

The invention discloses an application of a nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants, and particularly relates to the application taking the nitrogen-doped carbon point (N-CDs) as a matrix of a matrix-assisted laser desorption ionization-time of flight time mass spectrometry (MALDI-TOF MS) to the analysis of the micro-molecular environmental pollutants. By taking the nitrogen-doped carbon point as the matrix, the analysis and detection on the micro-molecular environmental pollutants with the m/z ranging from 150 to 1000 can be efficiently and rapidly realized. Suitable micro-molecule types comprise hydroxyl polycyclic aromatic hydrocarbon, nitryl polycyclic aromatic hydrocarbon, bisphenol compounds, perfluoro-acid environmental pollutants and the like. When the nitrogen-doped carbon point is used as the matrix to detect micro-molecules, background interferences of a traditional organic matrix ion peak can be effectively eliminated; an ionization agent does not need to be added and the requirements on sample treatment are reduced; the nitrogen-doped carbon point has good reproducibility and high salt resistance; and an efficient and rapid detection method is provided for the environmental pollutants and an application range of the carbon point is expanded.

Description

technical field [0001] The invention relates to the use of nitrogen-doped carbon dots, in particular to the application of nitrogen-doped carbon dots as a MALDI-TOFMS matrix in the analysis of small molecule environmental pollutants. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS), since first reported by Karas et al., has become an effective tool for the analysis of non-volatile macromolecules such as proteins, peptides, carbohydrates, oligonucleotides , oligosaccharides, synthetic polymers, etc. Its superior advantages include simple sample preparation, soft ionization, high sensitivity, good salt tolerance, fast and high efficiency. These features make MALDI-TOFMS more and more popular in sample detection and identification. Although it performs well in the analysis of high molecular weight compounds, due to the traditional organic matrices such as α-cyano-4-hydroxy-cinnamic acid (CHCA), 2,5-dihydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 路雯婧李勇董川双少敏蔡宗苇
Owner SHANXI UNIV
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