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Construction method of light-assisted bipolar self-energized aptamer sensor for detecting lincomycin

An aptamer sensor and construction method technology, applied in the field of electrochemical biosensing, can solve the problems of bacterial drug resistance, large scale of instruments, health problems, etc., achieve sensitive detection, reduce production costs, and avoid the use of biomass Effect

Active Publication Date: 2021-09-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, residual LIN in food of animal origin may cause health problems such as allergic reactions and bacterial resistance
At present, the classic methods used to detect LIN include high performance liquid chromatography, chemiluminescence and fluorescence methods. Although these methods have high detection accuracy, they all have disadvantages such as complicated operation, long time-consuming and large-scale instruments.

Method used

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  • Construction method of light-assisted bipolar self-energized aptamer sensor for detecting lincomycin
  • Construction method of light-assisted bipolar self-energized aptamer sensor for detecting lincomycin
  • Construction method of light-assisted bipolar self-energized aptamer sensor for detecting lincomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) TiO 2 / NG preparation

[0046] Disperse 100 mg of glycine and 240 mg of titanium sulfate into 10 mL of graphene oxide aqueous dispersion (1.0 mg / mL), and sonicate for 2 h; transfer the formed suspension to a stainless steel autoclave, react at 180 ° C for 12 h, and Cool to room temperature, centrifuge, and wash the resulting precipitate three times with ultrapure water and ethanol; finally, freeze-dry to obtain TiO 2 / NG functional nanocomposites. Monomer TiO 2 It is prepared under the same conditions as above without adding glycine and graphene oxide.

[0047] (2) ZnPc / MoS 2 preparation of

[0048] Disperse 5.0mg of molybdenum disulfide and 3.0mg of zinc phthalocyanine powder into a mixed solution containing 7mL of water and 3mL of tert-butanol; place the above solution under an ultrasonic cell disruptor, and ultrasonically break it for 6 hours to obtain uniform zinc phthalocyanine Nanoparticle-modified molybdenum disulfide nanosheet suspension, namely ZnPc / M...

Embodiment 2

[0061] (1) TiO 2 / NG preparation

[0062]Disperse 50 mg of glycine and 120 mg of titanium sulfate into 5 mL of graphene oxide aqueous dispersion (1.0 mg / mL), and sonicate for 1 h; transfer the formed suspension to a stainless steel autoclave, react at 160 °C for 10 h, and Cool to room temperature, centrifuge, and wash the resulting precipitate three times with ultrapure water and ethanol; finally, freeze-dry to obtain TiO 2 / NG functional nanocomposites. Monomer TiO 2 It is prepared under the same conditions as above without adding glycine and graphene oxide.

[0063] (2) ZnPc / MoS 2 preparation of

[0064] Disperse 4.0mg of molybdenum disulfide and 2.0mg of zinc phthalocyanine powder into a mixed solution containing 6mL of water and 2mL of tert-butanol; place the above solution under an ultrasonic cell disruptor, and ultrasonically break it for 4 hours to obtain uniform zinc phthalocyanine Nanoparticle-modified molybdenum disulfide nanosheet suspension, namely ZnPc / MoS ...

Embodiment 3

[0067] (1) TiO 2 / NG preparation

[0068] Disperse 150 mg of glycine and 360 mg of titanium sulfate into 15 mL of graphene oxide aqueous dispersion (1.0 mg / mL), and sonicate for 3 h; transfer the formed suspension to a stainless steel autoclave, react at 200 ° C for 14 h, and Cool to room temperature, centrifuge, and wash the resulting precipitate three times with ultrapure water and ethanol; finally, freeze-dry to obtain TiO 2 / NG functional nanocomposites. Monomer TiO 2 It is prepared under the same conditions as above without adding glycine and graphene oxide.

[0069] (2) ZnPc / MoS 2 preparation of

[0070] Disperse 6.0mg of molybdenum disulfide and 4.0mg of zinc phthalocyanine powder into a mixed solution containing 8mL of water and 4mL of tert-butanol; place the above solution under an ultrasonic cell disruptor, and perform ultrasonic crushing for 8 hours to obtain uniform zinc phthalocyanine Nanoparticle-modified molybdenum disulfide nanosheet suspension, namely Zn...

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Abstract

The invention provides a construction method of a photo-assisted bipolar self-energized aptamer sensor, which is used for rapidly, simply, economically and sensitively detecting lincomycin (LIN), and comprises the following steps: 1, preparing a photo-anode material nitrogen-doped graphene-loaded titanium dioxide (TiO2 / NG) through adoption of a one-step hydrothermal method, preparing a photocathode material molybdenum disulfide nanosheet loaded zinc phthalocyanine (ZnPc / MoS2) by a cosolvent method without chemical reaction; and 2, constructing the light-assisted bipolar self-energized aptamer sensor for detecting the LIN. The light-assisted bipolar self-energized adapter sensor constructed by the invention does not need an external power supply, realizes two-dimensional energy conversion from light energy and chemical energy to electric energy, and has unique advantages in the aspect of pushing miniature and convenient sensing equipment. Meanwhile, the cathode and the anode of the sensor are both made of photosensitive semiconductor materials, so that the use of biomasses such as enzymes and microorganisms and a noble metal Pt electrode is avoided, the solar energy utilization efficiency is greatly improved, and the manufacturing cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensing, and relates to a construction method of a light-assisted bipolar fuel cell-based self-powered aptamer sensor for detecting lincomycin. Background technique [0002] Self-powered electrochemical biosensor is a new type of electrochemical biosensing detection device, which has shown good application prospects in the fields of life analysis, environmental monitoring and food safety detection. Compared with traditional electrochemical biosensors, its biggest feature is that it does not require an external energy supply device (battery or power supply), by converting the concentration change of the target into the change of the power signal (such as open circuit voltage, output current or power, etc.) , and realize the sensing application according to the correlation between the two. Self-powered electrochemical biosensors take full advantage of the energy conversion in electrochem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26H02J7/35
CPCG01N27/30G01N27/305G01N27/26H02J7/35Y02P80/20Y02E60/50
Inventor 王坤张萌戴震
Owner JIANGSU UNIV
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