Production method and application of needle-point graphene electrochemical electrode

A kind of ene electrochemistry and electrochemistry technology, which is applied in the preparation and application field of needle-point graphene electrochemical electrodes, and achieves the effects of simple preparation process, easy functional modification and small size

Inactive Publication Date: 2012-09-26
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still few reports on the use of graphene combined with micro-nano tips to directly prepare electrochemical electrodes and study their application in the field of electrochemical biosensing after functionalization.

Method used

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  • Production method and application of needle-point graphene electrochemical electrode
  • Production method and application of needle-point graphene electrochemical electrode
  • Production method and application of needle-point graphene electrochemical electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Direct transfer of graphene films synthesized by chemical vapor deposition onto glass tip surfaces;

[0031] Use 0.5 mg of conductive silver glue to connect one end of the graphene film to one end of the metal wire. After the silver glue is cured for 15 minutes, a non-functional needle-point graphene electrochemical electrode can be obtained;

[0032] The tip diameter of the glass needle tip is 5.0 µm.

[0033] The metal wire is made of copper.

[0034] figure 1 Schematic diagram of the structure of the needle-tip graphene electrochemical electrode prepared by the above method; figure 2 Comparison of the cyclic voltammetry curves of the needle-tip graphene electrochemical electrode prepared by the above method and that of the gold nanoelectrode.

Embodiment 2

[0036] Direct transfer of graphene films synthesized by chemical vapor deposition onto glass tip surfaces;

[0037] Connect one end of the graphene film to one end of the metal wire with 0.5 mg of conductive silver glue, and obtain a non-functional needle-point graphene electrochemical electrode after the silver glue is cured for 15 minutes;

[0038] The tip diameter of the glass needle tip is 1.0 μm.

[0039] The metal wire material is titanium.

[0040] A 10.0 mmol cobalt nitrate aqueous solution was used as the electrolyte, and a layer of cobalt hydroxide was deposited on the surface of the needle-tip graphene by electrochemical deposition at a constant potential of -1.0 volts for 60 seconds.

[0041] After the graphene tip with cobalt hydroxide deposited on the surface is washed and dried, it is placed in a muffle furnace and baked at 400° C. for 2 hours to obtain a functionalized tip-type graphene electrochemical electrode modified by tricobalt tetroxide.

[0042] ima...

Embodiment 3

[0044] Direct transfer of graphene films synthesized by chemical vapor deposition onto glass tip surfaces;

[0045] Connect one end of the graphene film to one end of the metal wire with 0.5 mg of conductive silver glue, and obtain a non-functional needle-point graphene electrochemical electrode after the silver glue is cured for 15 minutes;

[0046] The tip diameter of the glass needle tip is 1.0 μm.

[0047] The metal wire is made of copper.

[0048] A 10.0 mmol cobalt nitrate aqueous solution was used as the electrolyte, and a layer of cobalt hydroxide was deposited on the surface of the needle-tip graphene by electrochemical deposition at a constant potential of -1.0 volts for 60 seconds.

[0049] After the graphene tip with cobalt hydroxide deposited on the surface is washed and dried, it is placed in a muffle furnace and baked at 400° C. for 2 hours to obtain a functionalized tip-type graphene electrochemical electrode modified by tricobalt tetroxide.

[0050] Figure...

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Abstract

The invention discloses a production method and application of a needle-point graphene electrochemical electrode. The production method includes the steps of transferring a graphene film synthesized by chemical vapor deposition to the surface of a micro-nano glass needle point, and using liquid conductive silver glue to connect the graphene and a lead to obtain a needle-point graphene electrode; using cobalt nitrate aqueous solution as electrolyte to deposit cobalt hydroxide on the surface of the needle-point graphene electrode by electrochemical deposition, and heating in a muffle furnace at 300-500 DEG C for 2-4 hours to obtain the needle-point graphene electrochemical electrode functionally modified by tricobalt tetroxide. The needle-point graphene electrochemical electrode can be used to detect micro-droplet samples. After surface modification by tricobalt tetroxide, a high-sensitivity electrochemical biosensor available for enzyme-free detection of glucose is obtained. In addition, the needle-point graphene electrochemical electrode can be used to puncture an active cell to detect intracellular metabolic process in real time.

Description

technical field [0001] The invention relates to a preparation method of a needle-point graphene electrochemical electrode for electrochemical biosensing and its application in the field of electrochemical biosensing. Background technique [0002] Electrochemical biosensors play a vital role in today's highly sensitive biomolecular detection, clinical medicine and genetic engineering research fields. Among them, the analysis and detection of micro-droplet samples by electrochemical electrodes and the real-time detection of intracellular metabolic processes have become a hot spot in the field of biosensing research, and the improvement and improvement of the performance of electrochemical electrodes is very important for the expansion of electrochemical biosensors in micro-samples. The application in the field of analysis and detection and ensuring its stable operation play a decisive role. [0003] Graphene is a two-dimensional honeycomb crystal structure composed of a singl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
Inventor 董晓臣黄维赵强汪联辉刘淑娟魏昂
Owner NANJING UNIV OF POSTS & TELECOMM
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