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Capillary action-based in situ sampling-microextraction device for allelochemicals in soil

A technology of capillary action and extraction device, which is applied in the direction of sampling device, measuring device, instrument, etc.

Inactive Publication Date: 2019-02-15
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the in situ sampling technology of soil solution has hardly been applied in the ecological field of allelopathic research, it has become an important research method in soil, plant nutrition, ecological environment and other disciplines. How to effectively extract soil solution has become a important technical issues

Method used

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  • Capillary action-based in situ sampling-microextraction device for allelochemicals in soil
  • Capillary action-based in situ sampling-microextraction device for allelochemicals in soil

Examples

Experimental program
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Effect test

Embodiment 1

[0029] like figure 1 , figure 2 As shown, the drill bit 1 is connected to the sampling tube 2 through threads, and the sealed double-ended stud 3 at the other end of the sampling tube 2 is connected to the tail pipe 5, and the outer end of the tail pipe 5 is provided with a sealing end cap 6; when applied to solid-phase microextraction , in-situ sampling of soil allelochemicals based on capillary action-a small hole is reserved on the sealing end cover 6 of the micro-extraction device, and the channel 4 set in the middle of the tail pipe 5 can allow the solid-phase extraction fiber 9 to pass through; During phase microextraction, in-situ sampling of soil allelochemicals based on capillary action-microextraction device puts a bent U-shaped hollow fiber 7 in the sampling tube 2, and connects the micro sample injector 8 during extraction. At this time, the channel 4 Used as an insertion channel for the cleaning syringe 11. Among them, the sampling pipe 2 is not only a collecto...

Embodiment 2

[0033]Taking capsicum as a model plant, the in-situ sampling-microextraction device based on capillary action of soil allelochemicals was placed in five pots A, B, C, D, and E, and pot E was the soil where no capsicum was planted, as a treatment control . Three days before the transplanting of the seedlings, add 2L of water per pot per day, and then add 1L of water per day to help the tubes absorb the soil solution. After continuous culture and collection for three weeks, sampling experiment: solid phase microextraction, the solid phase The extraction fiber 9, under the protection of the micro-extraction protection needle tube 10, passes through the rubber double-ended stud 3, inserts the sampling tube 2 and extends the extraction fiber 9, after the extraction is completed, shrinks back to the extraction fiber 9, and pulls out the micro-extraction The device is directly used for sample injection of instruments such as GC-MS. The small hole on the sealing end cap 6 is blocked b...

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Abstract

The invention provides a capillary action-based in situ sampling-microextraction device for allelochemicals in soil, and belongs to the technical field of plant root exudates and allelochemicals sampling. In the invention, the drill bit is connected to one end of a sampling tube through threads, the tube wall of the sampling tube has a micro hole. A double-head stud is a sealing rubber double-sided thread cap at the other end of the sampling tube. The other end of the sampling tube is connected to one end of the double-head stud through the threads, and the other end of the double-head stud isconnected to a tail tube through the threads. A channel is arranged in the middle of the tail tube, and a sealed end cover is arranged at the outer end of the tail tube. The sampling tube is made ofhigh-wetting alumina, and is repeatedly heated and sintered into a microporous ceramic sampling tube with a capillary diameter of 0.05-100um. The in situ sampling-microextraction device has strong capillary action, and liquid flows into the tube from micropores, which facilitates periodic microextraction, and qualitative and quantitative analysis of the allelochemicals in the soil.

Description

technical field [0001] The invention relates to an in-situ sampling-microextraction device for soil allelochemicals based on capillary action, and belongs to the technical field of sampling plant root exudates and allelochemicals. Background technique [0002] The release pathways of plant allelochemicals mainly include: root secretion to the outside world, plant rain and fog leaching and volatilization; plant or residue decomposition under the action of soil physical and chemical factors and microorganisms; pollen dispersal and seed germination. In order to study the impact of environmental factors such as root exudates species and concentration, stress on root exudates, allelochemical substances and allelopathic effects in root exudates, people have designed many collection methods for root exudates, mainly containing: solution Culture collection, matrix culture (vermiculite culture, sand culture, agar culture) collection, soil culture collection, automatic continuous coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/08G01N30/06
CPCG01N1/08G01N30/06G01N2030/062
Inventor 王炎赵敏王宁
Owner HARBIN INST OF TECH
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