Dispersed solid-phase extraction material, preparation method and application thereof

Abstract

The invention discloses a dispersed solid-phase extraction material, a preparation method and application thereof, and belongs to the technical field of the detection of aflatoxin in edible oil. The preparation method of the dispersed solid-phase extraction material includes the following steps that: humic acid is weighed and is subjected to ultrasonic washing for 12 to 18 min, and the washed humic acid is subjected to centrifuging, and a solid precipitate is obtained and is dispersed in acetone, and the acetone is subjected to heating and evaporation until the acetone is completely evaporated, and residues are obtained; the residues are taken and arranged in a Soxhlet extractor, and 180 ml of cleaning fluid is added to the Soxhlet extractor, and heating, refluxing and cleaning are performed until a reflux liquid is clear and colorless, and heating is stopped; a cleaned material is taken out, and the material is dried at 100 DEG C for 1.5 to 2.5h, and is cooled for 0.5 to 1h, and thematerial is sieved by a 120-mesh screen, screen underflow is obtained, and the dispersed solid-phase extraction material is obtained. The preparation method disclosed by the invention is suitable formost existing humic acids on the market at present. The preparation method is simple in process and beneficial to control; and the prepared dispersed solid-phase extraction material is stable in property.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin detection in edible oil, and in particular relates to a dispersed solid-phase extraction material and a preparation method and application thereof. Background technique [0002] my country is not only a major producer of edible oil but also the largest consumer of edible oil in the world. The annual per capita consumption of edible oil in my country is increasing year by year with the continuous improvement of living standards. Therefore, "edible oil safety" is an important part of "food safety", not only related to the health of the national physique, but also directly affects the development of the national economy. [0003] Aflatoxins (AFs) are a major pollutant of edible oil, which are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. So far, a total of 18 AFs have been reported, of which AFB 1 、AFB 2 、AFG 1 and AFG 2 are the four most common. AFs have the charac...

AI Technical Summary

Problems solved by technology

However, these sample pretreatment methods will face the following problems when detecting AFs in edible oil in large quantities: 1. Liquid-liquid extraction will consume a large amount of organic reagents and have a great impact on the recovery of the method; 2. Extraction Separated from the purification steps, the operation process is cumbersome and time-consuming; 3. The IAC column and MFC column used in sample pretreatment are expensive

Based on this, the current food safety testing industry is facing problems such as cumbersome sample pretreatment methods, high testing costs, and poor stability when testing AFs in edible oils in large quantities.

In order to evaluate, monitor and ensure the safety of edible oil, a large number of AFs in local edible oil are tested every year all over the country. Using the above sample pretreatment method will undoubtedly consume a lot of time and manpower, and at the same time consume huge funds

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