On-line digestion combined liquid chromatography system and method for detecting coloring agent in tea

Abstract

The invention provides an on-line digestion combined liquid chromatography system and a method for detecting a coloring agent in tea. The on-line digestion combined liquid chromatography system comprises a first runner, a second runner, a waste liquid runner, an analysis runner, a switching valve and the like and further comprises a digestion runner, a digestion liquid runner, a buffering runner, an extraction runner and the like. The on-line digestion combined liquid chromatography system can realize on-line extraction of coloring agents to be digested in tea or other solid substrates without tedious one-line digestion, extraction and concentration processes, and achieves integration and high automation degree of extraction and liquid chromatographic analysis, so that the working efficiency is greatly improved and time is saved. The method provided by the invention is used for on-line detection of other coloring agents which do not need digestion in tea, and different coloring agents can be detected by the same equipment.

Description

technical field [0001] The invention belongs to the field of liquid chromatographic systems, and in particular relates to an on-line digestion combined liquid chromatographic system and a detection method for colorants in tea. Background technique [0002] Liquid chromatography has the advantages of high detection sensitivity, high separation efficiency, high selectivity, low detection limit, less sample consumption, convenience and quickness, etc., especially combined with mass spectrometry detector, which greatly improves detection sensitivity and anti-interference ability, and has been widely used In the safety testing of the food industry, such as the detection of natural toxins, pesticides, food additives, veterinary drugs, etc. in food. [0003] Generally, no coloring agents are added to tea, but some unscrupulous traders will add some coloring agents to improve the color and appearance of tea, such as phthalocyanine blue, phthalocyanine red, phthalocyanine green G and...

AI Technical Summary

Problems solved by technology

Doing so requires a long manual process, which is cumbersome, time-consuming and labor-intensive

[0005] In addition, the above extraction process requires extraction containers, heating appliances, ultrasonic instruments, vibration instruments and other auxiliary instruments. After the sample is processed, it needs to be manually transferred to the liquid chromatograph for analysis, and the complete integration of sample processing and liquid chromatography cannot be realized. The on-line determination of liquid chromatography greatly reduces the automation of liquid chromatography, which in turn affects the efficiency and speed of batch sample analysis.

In addition, due to the instability of the chemical structure, some target components are prone to oxidation, volatilization, decomposition and other problems, so the problem of target loss may occur during the transfer process, thus affecting the accuracy of the entire liquid chromatographic determination

[0006] In short, there is no liquid chromatography system that can realize on-line digestion and analysis in the prior art, and there is no detection method for coloring agents in tea leaves. It is of great significance to develop such a system and method

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