A kind of dispersed solid phase extraction material and its preparation method and application

A technique of dispersing solid phase and extraction, which is applied to the analysis of materials, material separation, and material inspection products, etc. It can solve the problems of cumbersome operation process, high detection cost, and large expenditure, and achieve broad application prospects, low matrix interference, and guaranteed quality Effect

Active Publication Date: 2020-10-16
GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these sample pretreatment methods will face the following problems when detecting AFs in edible oil in large quantities: 1. Liquid-liquid extraction will consume a large amount of organic reagents and have a great impact on the recovery of the method; 2. Extraction Separated from the purification steps, the operation process is cumbersome and time-consuming; 3. The IAC column and MFC column used in sample pretreatment are expensive
Based on this, the current food safety testing industry is facing problems such as cumbersome sample pretreatment methods, high testing costs, and poor stability when testing AFs in edible oils in large quantities.
In order to evaluate, monitor and ensure the safety of edible oil, a large number of AFs in local edible oil are tested every year all over the country. Using the above sample pretreatment method will undoubtedly consume a lot of time and manpower, and at the same time consume huge funds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of dispersed solid phase extraction material and its preparation method and application
  • A kind of dispersed solid phase extraction material and its preparation method and application
  • A kind of dispersed solid phase extraction material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The application provides a method for preparing a dispersed solid phase extraction material, comprising the following steps:

[0038]S1. Weigh 5g of the humic acid sample sold in China and put it into a 1L beaker, add 500mL of water, fully stir and disperse, put the beaker into an ultrasonic instrument, and perform ultrasonic cleaning. The time of ultrasonic cleaning is 15min. Set aside for 8 hours, remove the supernatant, add 500mL of water again, perform ultrasonic cleaning for 15 minutes, let stand for 8 hours, remove the supernatant, repeat the above steps 10 times, until the supernatant has no obvious light yellow phenomenon under natural light; If the particles in the beaker are not completely settled after standing for 8 hours during the standing process of the sample, repeat the above steps for 10 times of cleaning; Min speed, centrifuge for 6min, centrifuge to obtain solid precipitate, transfer the precipitate to a 75mL evaporating dish with acetone, heat the e...

Embodiment 2

[0043] The application provides a method for preparing a dispersed solid phase extraction material, comprising the following steps:

[0044] S1. Weigh 5g of the humic acid sample sold in China and put it into a 1L beaker, add 500mL of water, fully stir and disperse, put the beaker into an ultrasonic instrument, and perform ultrasonic cleaning. The time of ultrasonic cleaning is 12min. Set aside for 8 hours, remove the supernatant, add 500mL of water again, perform ultrasonic cleaning for 12 minutes, let stand for 8 hours, remove the supernatant, repeat the above steps 10 times, until the supernatant has no obvious light yellow phenomenon under natural light; If the particles in the beaker are not completely settled after standing for 8 hours during the standing process of the sample, repeat the above steps for 10 times of cleaning; Min speed, centrifuge for 6min, centrifuge to obtain solid precipitate, transfer the precipitate to a 75mL evaporating dish with acetone, heat the ...

Embodiment 3

[0049] The application provides a method for preparing a dispersed solid phase extraction material, comprising the following steps:

[0050] S1. Weigh 5g of the humic acid sample sold in China and put it into a 1L beaker, add 500mL of water, fully stir and disperse, put the beaker into an ultrasonic instrument, and perform ultrasonic cleaning. The time of ultrasonic cleaning is 16min. Leave for 7 hours, remove the supernatant, add 500mL of water again, perform ultrasonic cleaning for 16 minutes, let stand for 7 hours, remove the supernatant, repeat the above steps 10 times, until the supernatant has no obvious light yellow phenomenon under natural light; If the particles in the beaker are not completely settled after standing for 8 hours during the standing process of the sample, repeat the above steps for 10 times of cleaning; Min speed, centrifuge for 6min, centrifuge to obtain solid precipitate, transfer the precipitate to a 75mL evaporating dish with acetone, heat the evap...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a dispersed solid phase extraction material and its preparation method and application, belonging to the technical field of aflatoxin detection in edible oil. The preparation method of the dispersed solid phase extraction material comprises the following steps: weighing humic acid, washing with ultrasonic waves 12‑18min, after washing with water, centrifuge to take the solid precipitate, disperse it in acetone, heat and evaporate until the acetone is completely evaporated, and obtain a residue; take the residue, put it in a Soxhlet extractor, add 180mL of cleaning solution, and heat and reflux to wash until After the reflux liquid is clear and colorless, stop heating; take out the cleaned material, dry the material at 100°C for 1.5-2.5h, cool it for 0.5-1h, and then pass through a 120-mesh sieve, take the under-sieved material, and obtain the dispersed solid phase extraction material. The preparation method of the present invention is applicable to most humic acids currently on sale, and the preparation method has simple process, is beneficial to control, and the properties of the dispersed solid-phase extraction material after cleaning and preparation are stable.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin detection in edible oil, and in particular relates to a dispersed solid-phase extraction material and a preparation method and application thereof. Background technique [0002] my country is not only a major producer of edible oil but also the largest consumer of edible oil in the world. The annual per capita consumption of edible oil in my country is increasing year by year with the continuous improvement of living standards. Therefore, "edible oil safety" is an important part of "food safety", not only related to the health of the national physique, but also directly affects the development of the national economy. [0003] Aflatoxins (AFs) are a major pollutant of edible oil, which are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. So far, a total of 18 AFs have been reported, of which AFB 1 、AFB 2 、AFG 1 and AFG 2 are the four most common. AFs have the charac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062G01N33/50G01N2333/38G01N2030/027
Inventor 唐阳廖艳华刘平雷宁生梁川
Owner GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap