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A kind of bergamot flavone extract and its separation and purification method

A technology of bergamot flavonoids and extracts, which is applied in the field of separation and purification, can solve the problems of low extraction rate, many impurities, and generate a large amount of acid-base solution, and achieves the effects of large extraction amount and high extraction efficiency.

Active Publication Date: 2021-12-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, there are many impurities easily soluble in water during hot water extraction, the post-treatment is complicated, and the extraction efficiency is not high; the solvent consumption of the organic solvent extraction method is large, the time is long, and the extraction rate is low; the alkaline water extraction method has high leaching ability, but the impurities More, not conducive to purification, and will produce a large amount of acid-base solution, causing environmental pollution

Method used

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  • A kind of bergamot flavone extract and its separation and purification method
  • A kind of bergamot flavone extract and its separation and purification method
  • A kind of bergamot flavone extract and its separation and purification method

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Effect test

Embodiment 1

[0043] Embodiment 1 A kind of method for separating and purifying flavone extract from Guangfo hands

[0044] 1. Purification method

[0045] (1) Preparation of the crude extract of bergamot flavonoids: 400g of bergamot flavonoids was extracted by continuous phase change extraction. The extraction conditions were: ethanol concentration 85%, extraction temperature 90°C, extraction time 120min, flow rate 20L / h;

[0046] (2) Polyamide resin pretreatment: Take an appropriate amount of polyamide resin and soak it in 95% ethanol for 24 hours, stir continuously, remove air bubbles, and put it into the column. Elute with 3 column volumes of 95% ethanol until the eluate is transparent and there is no residue (or very little residue) after evaporation to dryness. Then sequentially elute with 2.5 times column volume of 5% NaOH aqueous solution, 1 times column volume of distilled water, and 2.5 times column volume of 10% acetic acid aqueous solution, and finally elute with distilled wat...

Embodiment 2

[0059] Example 2 Influence of sample loading flow rate on polyamide adsorption effect

[0060] Pretreated polyamide resin 5g (on a dry basis) is packed into a glass chromatography column (26×400 mm) by a wet method, the column volume is 20mL, and the sample loading is 2.087mg / mL (flavonoid concentration) prepared in Example 1 The crude extract of flavonoids was loaded at 1.0mL / min, 1.5mL / min, and 2.0mL / min respectively for dynamic adsorption, and the effluent was collected according to the column bed volume (BV) (BV is the volume multiple of the resin), and each The concentration of total flavonoids in bergamot in part of the effluent was detected, and the concentration of flavonoids was detected, and the flow rate was plotted against the concentration of the effluent to investigate the effect of sample loading flow rate on the adsorption effect. result Figure 4 shown. Depend on Figure 4 It can be seen that when the sample loading flow rate is 1 mL / min, the time to reach ...

Embodiment 3

[0061] Example 3 The influence of sample loading volume on polyamide adsorption effect

[0062] Pretreated polyamide resin 5g (on a dry basis) is packed into a glass chromatography column (26×400 mm) by a wet method, the column volume is 20mL, and the sample loading is 2.087mg / mL (flavonoid concentration) prepared in Example 1 The crude extract of flavonoids, the loading flow rate is 1mL / min, the direction flows through the resin bed from top to bottom, the effluent is collected according to the column bed volume (BV) (BV is the resin volume multiple), and the total amount of bergamot in each effluent is measured. Concentration of flavonoids, taking the concentration of total flavonoids in the effluent as one-tenth of the concentration of the sample solution as the leak point, taking the volume of the effluent (BV) as the abscissa, the percentage of the total flavonoids in the effluent and the total flavonoids in the sample solution Values ​​(C / C0 x 100%) were used to plot the...

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Abstract

The invention provides a bergamot flavonoid extract and a separation and purification method thereof. The invention uses bergamot powder as a raw material, adopts continuous phase change extraction equipment to obtain bergamot flavonoid crude extract, and then adopts polyamide column chromatography to separate and purify the bergamot flavonoid crude extract to obtain bergamot flavonoid extract. The method can efficiently enrich and purify flavonoids, the total flavonoid content in the obtained 30% ethanol eluted fraction is >80%, and the total flavonoid content in the 60% ethanol eluted fraction is >50%, and the obtained flavonoid extract is rich in components, Rich in eriocitrin, hesperidin, rutin naringin, diosmin, narcissin, kaempferol‑3‑O‑rutinoside, and rutin. The invention provides an efficient, green and environment-friendly method capable of effectively retaining and enriching the functional components of the flavonoid extract, and provides a new way for the comprehensive utilization of bergamot.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a bergamot flavonoid extract and a separation and purification method thereof. Background technique [0002] Bergamot is the dry fruit of Citrus medica L.var.Sarcodactylis Swingle, also known as bergamot, bergamot citron, citron, etc. It has been carried on until now, and in 2012, it was added as a traditional Chinese medicine for both medicine and food. According to "Materia Medica Congxin", "Southern Yunnan Materia Medica", "Compendium of Materia Medica" and other records, bergamot has the effects of eliminating phlegm and stagnation, drying dampness and relieving cough. Clinically, bergamot is also used to treat common spleen and stomach diseases such as chronic gastritis, peptic ulcer, functional dyspepsia, and even acute severe pancreatitis. In my country, it is divided into Guangberg Bergamot, Sichuan Bergamot, Golden Bergamot, and Fujian Bergamot acco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/752
CPCA61K36/752A61K2236/333A61K2236/55
Inventor 周爱梅罗旭洸
Owner SOUTH CHINA AGRI UNIV
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