Detection method of fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet and obtained fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet

A technology of Qiling Jianshen tablets and fingerprints, applied in the field of quality control of traditional Chinese medicine preparations, to achieve the effects of objective conclusion, excellent stability and good reproducibility

Active Publication Date: 2018-03-09
JIANGSU KANION PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In the prior art, there is no public report on the finge

Method used

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  • Detection method of fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet and obtained fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet
  • Detection method of fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet and obtained fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet
  • Detection method of fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet and obtained fingerprint spectrum of astragalus membranaceus and poria cocos kidney invigoration tablet

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Effect test

Embodiment 1

[0069] Embodiment 1, a kind of detection method of fingerprint of Qiling Jianshen tablet adopts high performance liquid chromatography to detect, and its steps are as follows:

[0070] (1) Preparation of the test solution: get an appropriate amount of contents under the Qilingjianshen tablet loading difference item, mix well, grind finely, accurately weigh, place in a stoppered conical flask, and accurately add a volume concentration of 15mL of 30% methanol, weighed; ultrasonically treated for 15min, allowed to cool, weighed again, and supplemented with 30% methanol to make up the lost weight, shake well, filter, continue filtrate with microporous membrane filter, filtrate As the test solution;

[0071] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of chlorogenic acid reference substance, sinomenine reference substance, and icariin reference substance, and prepare a solution containing chlorogenic acid, icariin, and sinomenine with met...

Embodiment 2

[0075] Embodiment 2, a kind of detection method of fingerprint of Qiling Jianshen tablet adopts high performance liquid chromatography to detect, and its steps are as follows:

[0076] (1) Preparation of the test solution: get an appropriate amount of contents under the Qilingjianshen tablet loading difference item, mix well, grind finely, accurately weigh, place in a stoppered conical flask, and accurately add a volume concentration of 75mL of 70% methanol, weighed; ultrasonically treated for 60min, allowed to cool, weighed again, supplemented the lost weight with 70% methanol by volume concentration, shaked well, filtered, continued filtrate filtered with microporous membrane, filtrate As the test solution;

[0077] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of chlorogenic acid reference substance, sinomenine reference substance, and icariin reference substance, and prepare a solution containing chlorogenic acid, icariin, and sino...

Embodiment 3

[0081] Embodiment 3, a kind of detection method of fingerprint of Qiling Jianshen tablet adopts high performance liquid chromatography to detect, and its steps are as follows:

[0082] (1) Preparation of the test solution: take 0.35 g of the content of Qiling Jianshen Tablets under the item of loading difference, mix well, grind finely, accurately weigh, place in a stoppered conical flask, and accurately add volume concentration 50mL of 50% methanol, weighed; ultrasonically treated for 30min, allowed to cool, weighed again, supplemented the lost weight with 50% methanol by volume, shaken well, filtered, and took the filtrate to pass through a 0.22μm filter membrane, The filtrate is used as the test solution;

[0083] (2) Preparation of reference substance solution: Accurately weigh appropriate amounts of chlorogenic acid reference substance, sinomenine reference substance, and icariin reference substance, and use methanol to prepare chlorogenic acid 40 μg, icariin 40 μg, A mi...

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Abstract

The invention discloses a detection method of a fingerprint spectrum of an astragalus membranaceus and poria cocos kidney invigoration tablet and the fingerprint spectrum of the astragalus membranaceus and poria cocos kidney invigoration tablet. According to the method, a standard fingerprint spectrum of the astragalus membranaceus and poria cocos kidney invigoration tablet is established; high performance liquid chromatography and gradient elution are adopted; the number of theoretical plates is calculated according to a sinomenine peak and is not less than 100000. The similarity between thefingerprint spectrum of the astragalus membranaceus and poria cocos kidney invigoration tablet obtained by the method and a control fingerprint spectrum is greater than 0.9; the fingerprint spectrum can comprehensively and effectively characterize quality of the astragalus membranaceus and poria cocos kidney invigoration tablet; a quality evaluation system of the astragalus membranaceus and poriacocos kidney invigoration tablet is perfected; the obtained fingerprint spectrum of the astragalus membranaceus and poria cocos kidney invigoration tablet has many characteristic peaks and is high insimilarity; the quality of the astragalus membranaceus and poria cocos kidney invigoration tablet can be monitored accurately and reliably; the detected fingerprint spectrum is recognized by a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system provided by Chinese Pharmacopoeia Commission; the fingerprint spectrum of the astragalus membranaceus and poriacocos kidney invigoration tablet is evaluated according to an obtained similarity result; a conclusion is relatively objective and accurate; and the method has the advantages that the method is simpleand convenient, excellent in stability, high in precision, good in repeatability and the like.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine preparations, in particular to a method for detecting the fingerprints of Qiling Jianshen Tablets and the fingerprints of Qiling Jianshen Tablets obtained by the method. Background technique [0002] Since traditional Chinese medicines and their preparations are multi-component complex systems, the evaluation of their quality should adopt appropriate detection methods that can provide rich identification information. Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. At the present stage, when most of the active ingredients of traditional Chinese medicine are not clear, the fingerprint of traditional Chinese medicine is of great significance for effectively controlling the quality of...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 萧伟郎悦胡军华秦建平李东坡任伟李家春王振中
Owner JIANGSU KANION PHARMA CO LTD
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