44results about How to "Many characteristic peaks" patented technology

The invention provides an establishing method and a use of a flue-cured tobacco GC / MS fingerprint. The establishing method of the flue-cured tobacco GC / MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC / MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC / MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC / MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC / MS detection technology so that the flue-cured tobacco GC / MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC / MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

The invention discloses a preparation method of liquorice formula granules and a quality control method for quickly detecting finished liquorice formula granule products, raw medicinal materials and intermediate products. The liquorice formula granules are prepared by performing the technological processes such as water extraction, decompression concentration and spray drying on liquorice serving as a raw material to obtain liquorice spray-dried powder and directly drying the liquorice powder or adding a proper quantity of auxiliary materials for mixing; each gram of the liquorice formula granules is equal to 3 grams of raw medicines. According to the method, the liquorice formula granules or the raw medicinal martials or an ethyl alcohol extract of spray-dried powder are or is tabletted with potassium bromide to prepare a sample, and then a Fourier transformation infrared spectrometer is used for performing infrared measurement on tablets to obtain infrared characterization fingerprints to realize quick detection and quality control on the finished liquorice formula granule products, the raw medicinal materials and the intermediate products, so that the stability and the reliability of the liquorice formula granule production technology are guaranteed. The quality control method disclosed by the invention is scientific, advanced, quick, economical and specific and is high in operability.

The invention relates to a construction method and application of a standard characteristic chromatogram of a Chinese patent medicine, i.e., a pharyngitis-treating tablet. The construction method comprises the following steps: weighing control substances of (R,S)-goitrin, paeoniflorin and harpagoside, a sample of the pharyngitis-treating tablet, and 12 negative samples of the pharyngitis-treating tablet, wherein the 12 negative samples are prepared according to a prescription and preparation process for the pharyngitis-treating tablet and respectively lack in Radix Scrophulariae, isatis roots, Radix Asparagi, Radix Ophiopogonis, tree peony bark, Radix Stemonae Preparata, Chinese olive, Flos Farfarae Preparata, Oroxylum indicum, Rhizoma Rehmanniae, Periostracum Cicadae and peppermint oil; separately preparing control solutions, a test solution and negative sample solutions; precisely sucking up 1 [mu]L of each of the above solutions and injecting the sucked solutions into a liquid chromatograph; carrying out determination according to set chromatographic conditions so as to obtain corresponding chromatograms; and carrying out calculating by using a chromatographic fingerprint similarity evaluation system for traditional Chinese medicines so as to generate the standard characteristic chromatogram of the pharyngitis-treating tablet. The standard characteristic chromatogram of the pharyngitis-treating tablet constructed in the invention for the first time is complete in characteristic peaks, accurate in positioning and fast in construction method, can effectively identify the authenticity of the Chinese patent medicine and evaluate the quality of the Chinese patent medicine, and is expected to become a standard method for quality control of the Chinese patent medicine, i.e., the pharyngitis-treating tablet.

The invention relates to a method for constructing a refined coronary tablet specific chromatogram and measuring six components.Firstly, paeoniflorin, rosmarinic acid, salvianolic acid B, ligustilide, cryptotanshinone and a tanshinone IIA reference substance are acquired, 13 batches of refined coronary tablet samples are acquired, and four negative samples which are prepared according to the prescription proportion and process recorded in the Chinese Pharmacopoeia and lack the root of red-rooted salvia, the root of common peony, chuanxiong rhizome and flos carthami respectively are acquired; a reference substance solution, a test article solution and a negative sample solution are prepared in sequence, 0.5 microliter of each solution is precisely absorbed and injected into a liquid chromatograph for measurement so as to obtain a chromatogram, and calculation is conducted with a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system to obtain the specific chromatogram.The specific chromatogram constructed for the first time contains comprehensive characteristic peaks, positioning is accurate, the authenticity of the Chinese patent medicine is effectively identified, the quality of the Chinese patent medicine is effectively evaluated, multiple components of the refined coronary tablets can also be detected quickly, accurately and quantitatively, and the method is expected to become a standard method for quality control of the Chinese patent medicine refined coronary tablets.

The invention discloses a construction method and an application of a Shenzhiling oral liquid fingerprint. The method comprises the following steps: production of a sample solution, production of a reference substance solution, optimization and determination of chromatographic conditions, determination of the fingerprint, and analysis of the obtained fingerprint of a Shenzhiling oral liquid. Chromatographic columns having different stationary phases are directly connected in series, the same mobile phase is used to carry out gradient elution, and multiple characteristic index components are well separated. Compared with existing fingerprint establishing methods, the above method can realize the separation and detection of possible many characteristic components of the Shenzhiling oral liquid to obtain many fingerprint characteristic peaks, allows the component information of the Shenzhiling oral liquid to be comprehensively reflected, has the advantages of simplicity in operation, highprecision, good stability and good repeatability, can provide a more effective and comprehensive method basis for controlling and evaluating the quality of the Shenzhiling oral liquid, and ensures the effectiveness and the safety of drugs.

The invention discloses a method for establishing the HPLC fingerprint of a Liubanhong garlic enzymatic hydrolysis product. The method concretely comprises the following steps: carrying out solid phase extraction on bulbs of Liubanhong garlic which is a special kind in Baodi to prepare a test article solution, and carrying out high performance liquid chromatography analysis detection under certain conditions to obtain the HPLC fingerprint of the Liubanhong garlic enzymatic hydrolysis product. A standard fingerprint is obtained by determining common characteristic peaks HPLC fingerprint analysis of 10 batches of samples. The standard fingerprint can provide reliable bases for discrimination of the kind and the producing area of the Liubanhong garlic and production quality control. The method has the advantages of convenient operation, high stability, strong specificity, good reappearance, many characteristic peaks, high analysis efficiency, true and false and producing area discrimination of the Liubanhong garlic, and comprehensive, accurate and effective evaluation of the internal quality of the Liubanhong garlic.

The invention provides an establishing method of the fingerprint of a traditional Chinese medicinal composition, and a technology for detecting the components of the traditional Chinese medicinal composition by using the fingerprint established through the method. The raw materials of the traditional Chinese medicinal composition comprise Rhizoma Cibotii, Fructus Rosae Laevigatae, Caulis Spatholobi, Philippine flemingia root, Kadsura coccinea, Millettia specisoa Champ, steamed Ligustri Lucidi Fructus, steamed Chinese Taxillus Twig, brine processed semen cuscutae, processed Rhizoma Corydalis, Zanthoxylum nitidum, processed Boswellia carteri and processed myrrh. The establishing method of the fingerprint is characterized in that the retention time of every compound and every medicinal material in the traditional Chinese medicinal composition is detected through high performance liquid chromatography; and the conditions of the high performance liquid chromatography are as follows: octadecylsilane bonded silica is adopted as a stationary phase, a mobile phase comprises acetonitrile and an aqueous phosphoric acid solution with the weight percentage concentration of 0.1%, the volume percentage of the acetonitrile in the mobile phase uniformly increases to 90% from 5% at 0-170 min, the flow velocity is 1 ml / min, the column temperature is 30 DEG C, and the ultraviolet detection wavelength is 254 nm.

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of an allinase inactivated extract of six-peal red garlics. The establishment method comprises the following specific steps of preparing six-peal red bulbs which belong to a peculiar variety in Baodi County into a test product solution by a solid-phase extraction step and the like; performing HPLC detection under certain conditions to obtain the HPLC fingerprint spectrum of the allinase inactivated extract of the six-peal red garlics; analyzing the HPLC fingerprint spectrums of ten batches of samples to determine a common characteristic peak and obtaining a standard fingerprint spectrum which can provide a reliable basis for the identification of varieties and production places of the six-peal red garlics and control over the production quality. The establishment method disclosed by the invention has the advantages of convenience in operation, high stability, high specificity, good reproducibility, more characteristic peaks and high analytic efficiency; by using the establishment method disclosed by the invention, the authenticity and production place of the six-peal red garlics can be identified, and the internal quality of the six-peal red garlics can be evaluated comprehensively, accurately and effectively.

The invention provides a method for detecting hooker winghead root medicinal materials. According to the method, measurement is performed through UFLC (Ultra Fast Liquid Chromatography). According to the UFLC chromatographic detection method, chemical components in hooker winghead root can be effectively separated, the obtained chromatogram map contains multiple characteristic peaks, the similarity of fingerprint spectrums of medicinal materials in various batches is high, and the degree of separation, precision, stability and reproducibility are high. Moreover, the chromatographic detection time is within 20 minutes, the detection efficiency is high, and a new method is provided for detecting the quality of the hooker winghead root medicinal materials.

The invention relates to the Chinese medicine preparation quality control technology field and especially discloses the HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, a construction method and an application thereof. The construction method comprises the steps of the preparation of the solution of a testing product, comparison product solution preparation, the determination of an HPLC analytical chromatographic condition, the measurement of the fingerprint map spectrum and the like. Through comparing the HPLC fingerprint map spectrums of 10 batches of hawthorn broken wall decoction piece samples, a common peak is determined, and a standard HPLC fingerprint map spectrum is obtained. The construction method has the advantages of simple operation, good reproducibility, a lot of characteristic peaks, and accurate and reliable performance. The HPLC fingerprint map spectrum can comprehensively reflect the characteristic peak information of the sample, and the HPLC fingerprint map spectrum can be used for carrying out quality control on the hawthorn broken wall decoction pieces.

The invention provides a preparation method of morchella esculenta wine. The method comprises the following steps: firstly, treating fresh and clean morchella esculenta at a high pressure of 80-120MPa for 8-15min, then freezing the morchella esculenta at a temperature of -30 to -25 DEG C for 1-2 days, removing frost flakes from the surface of the morchella esculenta, and then soaking the morchella esculenta in basic liquor, thereby obtaining the morchella esculenta wine with a unique flavor and an extremely good mouthfeel. Then, a fingerprint chromatogram of the morchella esculenta wine is established by adopting high-performance liquid chromatography. The preparation method of the morchella esculenta wine is simple, the product is savory and mellow and keeps the scent for a long time, and the method can significantly prolong the discoloring time of the product and effectively keep the clarity and stability of a wine body. Meanwhile, the method for establishing the fingerprint chromatogram of the morchella esculenta wine is easy to implement, and the stability, reproducibility and specificity of the method are good, and can provide a reference for quality control of the morchella esculenta wine.

The invention provides a method for establishing a fingerprint spectrum of flavonoid components in a ginkgo leaf extraction intermediate or a preparation thereof and the fingerprint spectrum established by the method. The method for establishing the fingerprint spectrum comprises the step of detecting the relative retention time and / or the relative peak area of flavonoid components in the ginkgo leaf extraction intermediate or the preparation thereof by taking narcissoside as a reference and adopting a high performance liquid chromatography. The fingerprint spectrum comprises 23 common peaks. The invention also provides an application of the the method for establishing the fingerprint spectrum and / or the established fingerprint in true and false identification and component detection of the ginkgo leaf extraction intermediate and / or the preparation thereof.

The invention relates to the technical field of polypeptide quality standard, in particular to a method for detecting the quality of ossein peptide based on a spectrum-activity relationship. The method comprises the following steps: (1) establishing an HPLC (High-Performance Liquid Chromatography) fingerprint spectrum of a to-be-detected ossein peptide test sample; (2) then screening out characteristic chromatographic peaks; (3) determining an anti-inflammatory function of the to-be-detected ossein peptide test sample; (4) establishing spectrum-activity correlation analysis by adopting a greyrelational analysis method, thus evaluating the anti-inflammatory activity of a common peak. By adopting the method disclosed by the invention, a peak VII, a peak I and a peak V in ossein peptide havehigh relational degree with the anti-inflammatory activity; the method has the advantages that the method is simple, the objectivity is strong, more characteristic peaks are obtained, and the like, and the method is beneficial for carrying out quality control on the ossein peptide.

The invention provides an establishing method of the fingerprint of a traditional Chinese medicinal composition preparation, and a technology for detecting the components of the traditional Chinese medicinal composition preparation by using the fingerprint established through the method. The raw materials of the traditional Chinese medicinal composition preparation comprise Rhizoma Cibotii, Radix Kadsurae Coccineae,Philippine flemingia root, steamed Chinese Taxillus Twig, steamed Ligustri Lucidi Fructus, Caulis Spatholobi, Fructus Rosae Laevigatae, Radix millettiae Speciosae and brine processed semen cuscutae. The establishing method of the fingerprint is characterized in that the retention time of every compound in the traditional Chinese medicinal composition is detected through high performance liquid chromatography; and the conditions of the high performance liquid chromatography are as follows: octadecylsilane bonded silica is adopted as a stationary phase, a mobile phase comprises acetonitrile, methanol and a 0.1% aqueous phosphoric acid solution, gradient elution is carried out for 0-150 min, and the ultraviolet detection wavelength is 230 nm. The relative retention times and the relative peak areas of 43 common peaks in the fingerprint established through the method are calibrated by adopting the absorption peak of protocatechuic acid as a reference peak.

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

The invention discloses a construction method and application of a fingerprint of sweet dream oral liquid. The method comprises the following steps: preparing a test solution, preparing a reference solution, optimizing and determining chromatographic conditions, measuring the fingerprint and processing the obtained fingerprint by using fingerprint software. Chromatographic columns with different stationary phases are connected through a six-way valve; gradient elution is performed by the same mobile phase; and various characteristic index components are well separated. Compared with an existing established fingerprint method, the method achieves that characteristic components of the sweet dream oral liquid can be separated and detected as many as possible, obtains more fingerprint characteristic peaks, and more comprehensively reflects component information of the oral liquid. The method further has the advantages of being easy and convenient to operate, high in precision, good in stability and reproducibility and the like, and the quality of the sweet dream oral liquid can be evaluated more comprehensively and scientifically, so that the quality and the curative effect of the product are guaranteed, and reference is provided for perfection of the quality standard of the product.

The invention discloses an establishment method of inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints specifically comprises the steps of preparing inducing callus cultured Allium sativum L. bulbs into solution for test products through steps such as solid-phase extraction, and performing high-performance chromatographic analysis and detection under certain conditions to obtain HPLC fingerprints of inducing callus cultured Allium sativum L. enzymatic hydrolysate. By analyzing the HPLC fingerprints of 10 batches of samples, characteristic common peaks are determined and standard fingerprints are obtained. The standard fingerprints can provide a reliable basis for variety identification and production quality control of inducing callus cultured Allium sativum L. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints has the advantages that the operation is convenient, the stability is high, the specificity is strong, the repeatability is good, the characteristic peaks are more, the analysis efficiency is high, the truth identification of the inducing callus cultured Allium sativum L. can be realized by adopting the invention, and the interior product quality can be comprehensively, accurately and effectively evaluated.

The invention relates to a method for rapidly identifying traditional Chinese medicine formula particles employing a Fourier transform infrared spectrum, and belongs to the technical field of detection of traditional Chinese medicines. The method comprises the following steps: carrying out absolute ethyl alcohol extraction and ultrasonic extraction on traditional Chinese medicine formula particle samples, and filtering to obtain a sample solution; dropwise adding the sample solution to an agate mortar containing potassium bromide powder; stirring and drying at constant temperature, tabletting and preparing samples; measuring an infrared fingerprint by virtue of a Fourier transform infrared spectrometer; rapidly identifying the traditional Chinese medicine formula particles. According to the method, the defect of poor specificity under a conventional infrared tablet sample preparation method is overcome; rapid detection can be carried out; the result can be compared by virtue of a standard map or can be compared and identified by software similarity. The sample detected by an infrared fingerprint technology is an alcohol extraction concentrated liquor of formula particles; qualitative and semi-quantitative detection can be achieved; the method is easy, accurate, economical, good in repeatability, high in information content and high in spectral characteristics.

The invention provides a method for establishing a fingerprint of leech. The method comprises the following specific steps: preparing the leech into a test solution; and performing separation detection through a high performance liquid chromatograph under certain conditions to obtain the fingerprint of the leech. The invention also provides a standard fingerprint of the leech, which is obtained through the method. The fingerprint can provide a reliable basis for leech identification and internal quality control. The method is simple and convenient to operate, high in stability, high in repeatability and large in quantity of characteristic peaks, and is capable of comprehensively and accurately evaluating the quality of leech medicine.

The invention provides an establishing method and a use of a flue-cured tobacco GC / MS fingerprint. The establishing method of the flue-cured tobacco GC / MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC / MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC / MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC / MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC / MS detection technology so that the flue-cured tobacco GC / MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC / MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

The invention belongs to the field of traditional Chinese medicine preparation analysis, and particularly discloses a method for establishing a heat-clearing and detoxifying oral liquid HPLC fingerprint spectrum. The method comprises the following steps: preparing a reference substance solution and a test solution, determining HPLC chromatographic conditions and manufacturing an HPLC standard fingerprint spectrum. The invention also discloses the HPLC standard fingerprint spectrum of the heat-clearing and detoxifying oral liquid obtained by the method, and the fingerprint spectrum has 30 common peaks. The quality detection method disclosed by the invention is simple and convenient to operate, high in stability and good in reproducibility, obtained spectrum characteristic peaks are comprehensive, the quality of the heat-clearing and detoxifying oral liquid can be comprehensively evaluated and controlled by comparing common peaks of a standard fingerprint spectrum, the internal quality of a preparation can be accurately evaluated, and the safety and effectiveness of clinical medication are ensured.

The invention discloses an HPLC fingerprint detection method for liver-comforting decoction. According to the method, a high performance liquid chromatography under a specific condition is adopted tocarry out detection so as to obtain a fingerprint with 8 common peaks, so that the multi-component information of the products can be reflected. The method is simple, good in reproducibility and multiple in characteristic peaks, is capable of ensuring the quality uniformity and stability of the products and providing scientific basis for the quality control of the products.

The invention discloses a method for establishing the HPLC fingerprint of Tingli Dazao Xiefei Granules. The method comprises the preparation of the test solution, the determination of the HPLC chromatographic conditions and the preparation of the HPLC standard fingerprint. The invention also discloses the HPLC standard fingerprint spectrum of the Tinglidazao Xiefei granule obtained by the method, and the fingerprint spectrum has 27 common peaks. The quality detection method of the present invention is easy to operate, has high stability and good reproducibility, and the obtained spectrum has many characteristic peaks. Through the comparison of the common peaks of the standard fingerprint spectrum, the quality of Tingli Dazao Xiefei Granules can be comprehensively evaluated and controlled. It is beneficial to accurately evaluate the internal quality of preparations and ensure the safety and effectiveness of clinical medication.

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of an allinase inactivated extract of six-peal red garlics. The establishment method comprises the following specific steps of preparing six-peal red bulbs which belong to a peculiar variety in Baodi County into a test product solution by a solid-phase extraction step and the like; performing HPLC detection under certain conditions to obtain the HPLC fingerprint spectrum of the allinase inactivated extract of the six-peal red garlics; analyzing the HPLC fingerprint spectrums of ten batches of samples to determine a common characteristic peak and obtaining a standard fingerprint spectrum which can provide a reliable basis for the identification of varieties and production places of the six-peal red garlics and control over the production quality. The establishment method disclosed by the invention has the advantages of convenience in operation, high stability, high specificity, good reproducibility, more characteristic peaks and high analytic efficiency; by using the establishment method disclosed by the invention, the authenticity and production place of the six-peal red garlics can be identified, and the internal quality of the six-peal red garlics can be evaluated comprehensively, accurately and effectively.

The invention discloses a method for establishing the HPLC fingerprint of a Liubanhong garlic enzymatic hydrolysis product. The method concretely comprises the following steps: carrying out solid phase extraction on bulbs of Liubanhong garlic which is a special kind in Baodi to prepare a test article solution, and carrying out high performance liquid chromatography analysis detection under certain conditions to obtain the HPLC fingerprint of the Liubanhong garlic enzymatic hydrolysis product. A standard fingerprint is obtained by determining common characteristic peaks HPLC fingerprint analysis of 10 batches of samples. The standard fingerprint can provide reliable bases for discrimination of the kind and the producing area of the Liubanhong garlic and production quality control. The method has the advantages of convenient operation, high stability, strong specificity, good reappearance, many characteristic peaks, high analysis efficiency, true and false and producing area discrimination of the Liubanhong garlic, and comprehensive, accurate and effective evaluation of the internal quality of the Liubanhong garlic.