44results about How to "Many characteristic peaks" patented technology

The invention provides an establishing method and a use of a flue-cured tobacco GC/MS fingerprint. The establishing method of the flue-cured tobacco GC/MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC/MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC/MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC/MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC/MS detection technology so that the flue-cured tobacco GC/MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC/MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

The invention relates to a construction method and application of a standard characteristic chromatogram of a Chinese patent medicine, i.e., a pharyngitis-treating tablet. The construction method comprises the following steps: weighing control substances of (R,S)-goitrin, paeoniflorin and harpagoside, a sample of the pharyngitis-treating tablet, and 12 negative samples of the pharyngitis-treating tablet, wherein the 12 negative samples are prepared according to a prescription and preparation process for the pharyngitis-treating tablet and respectively lack in Radix Scrophulariae, isatis roots, Radix Asparagi, Radix Ophiopogonis, tree peony bark, Radix Stemonae Preparata, Chinese olive, Flos Farfarae Preparata, Oroxylum indicum, Rhizoma Rehmanniae, Periostracum Cicadae and peppermint oil; separately preparing control solutions, a test solution and negative sample solutions; precisely sucking up 1 [mu]L of each of the above solutions and injecting the sucked solutions into a liquid chromatograph; carrying out determination according to set chromatographic conditions so as to obtain corresponding chromatograms; and carrying out calculating by using a chromatographic fingerprint similarity evaluation system for traditional Chinese medicines so as to generate the standard characteristic chromatogram of the pharyngitis-treating tablet. The standard characteristic chromatogram of the pharyngitis-treating tablet constructed in the invention for the first time is complete in characteristic peaks, accurate in positioning and fast in construction method, can effectively identify the authenticity of the Chinese patent medicine and evaluate the quality of the Chinese patent medicine, and is expected to become a standard method for quality control of the Chinese patent medicine, i.e., the pharyngitis-treating tablet.

The invention provides an establishing method of the fingerprint of a traditional Chinese medicinal composition, and a technology for detecting the components of the traditional Chinese medicinal composition by using the fingerprint established through the method. The raw materials of the traditional Chinese medicinal composition comprise Rhizoma Cibotii, Fructus Rosae Laevigatae, Caulis Spatholobi, Philippine flemingia root, Kadsura coccinea, Millettia specisoa Champ, steamed Ligustri Lucidi Fructus, steamed Chinese Taxillus Twig, brine processed semen cuscutae, processed Rhizoma Corydalis, Zanthoxylum nitidum, processed Boswellia carteri and processed myrrh. The establishing method of the fingerprint is characterized in that the retention time of every compound and every medicinal material in the traditional Chinese medicinal composition is detected through high performance liquid chromatography; and the conditions of the high performance liquid chromatography are as follows: octadecylsilane bonded silica is adopted as a stationary phase, a mobile phase comprises acetonitrile and an aqueous phosphoric acid solution with the weight percentage concentration of 0.1%, the volume percentage of the acetonitrile in the mobile phase uniformly increases to 90% from 5% at 0-170 min, the flow velocity is 1 ml/min, the column temperature is 30 DEG C, and the ultraviolet detection wavelength is 254 nm.

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of an allinase inactivated extract of six-peal red garlics. The establishment method comprises the following specific steps of preparing six-peal red bulbs which belong to a peculiar variety in Baodi County into a test product solution by a solid-phase extraction step and the like; performing HPLC detection under certain conditions to obtain the HPLC fingerprint spectrum of the allinase inactivated extract of the six-peal red garlics; analyzing the HPLC fingerprint spectrums of ten batches of samples to determine a common characteristic peak and obtaining a standard fingerprint spectrum which can provide a reliable basis for the identification of varieties and production places of the six-peal red garlics and control over the production quality. The establishment method disclosed by the invention has the advantages of convenience in operation, high stability, high specificity, good reproducibility, more characteristic peaks and high analytic efficiency; by using the establishment method disclosed by the invention, the authenticity and production place of the six-peal red garlics can be identified, and the internal quality of the six-peal red garlics can be evaluated comprehensively, accurately and effectively.

The invention provides a method for detecting hooker winghead root medicinal materials. According to the method, measurement is performed through UFLC (Ultra Fast Liquid Chromatography). According to the UFLC chromatographic detection method, chemical components in hooker winghead root can be effectively separated, the obtained chromatogram map contains multiple characteristic peaks, the similarity of fingerprint spectrums of medicinal materials in various batches is high, and the degree of separation, precision, stability and reproducibility are high. Moreover, the chromatographic detection time is within 20 minutes, the detection efficiency is high, and a new method is provided for detecting the quality of the hooker winghead root medicinal materials.

The invention provides a preparation method of morchella esculenta wine. The method comprises the following steps: firstly, treating fresh and clean morchella esculenta at a high pressure of 80-120MPa for 8-15min, then freezing the morchella esculenta at a temperature of -30 to -25 DEG C for 1-2 days, removing frost flakes from the surface of the morchella esculenta, and then soaking the morchella esculenta in basic liquor, thereby obtaining the morchella esculenta wine with a unique flavor and an extremely good mouthfeel. Then, a fingerprint chromatogram of the morchella esculenta wine is established by adopting high-performance liquid chromatography. The preparation method of the morchella esculenta wine is simple, the product is savory and mellow and keeps the scent for a long time, and the method can significantly prolong the discoloring time of the product and effectively keep the clarity and stability of a wine body. Meanwhile, the method for establishing the fingerprint chromatogram of the morchella esculenta wine is easy to implement, and the stability, reproducibility and specificity of the method are good, and can provide a reference for quality control of the morchella esculenta wine.

The invention relates to the technical field of polypeptide quality standard, in particular to a method for detecting the quality of ossein peptide based on a spectrum-activity relationship. The method comprises the following steps: (1) establishing an HPLC (High-Performance Liquid Chromatography) fingerprint spectrum of a to-be-detected ossein peptide test sample; (2) then screening out characteristic chromatographic peaks; (3) determining an anti-inflammatory function of the to-be-detected ossein peptide test sample; (4) establishing spectrum-activity correlation analysis by adopting a greyrelational analysis method, thus evaluating the anti-inflammatory activity of a common peak. By adopting the method disclosed by the invention, a peak VII, a peak I and a peak V in ossein peptide havehigh relational degree with the anti-inflammatory activity; the method has the advantages that the method is simple, the objectivity is strong, more characteristic peaks are obtained, and the like, and the method is beneficial for carrying out quality control on the ossein peptide.

The invention provides an establishing method of the fingerprint of a traditional Chinese medicinal composition preparation, and a technology for detecting the components of the traditional Chinese medicinal composition preparation by using the fingerprint established through the method. The raw materials of the traditional Chinese medicinal composition preparation comprise Rhizoma Cibotii, Radix Kadsurae Coccineae,Philippine flemingia root, steamed Chinese Taxillus Twig, steamed Ligustri Lucidi Fructus, Caulis Spatholobi, Fructus Rosae Laevigatae, Radix millettiae Speciosae and brine processed semen cuscutae. The establishing method of the fingerprint is characterized in that the retention time of every compound in the traditional Chinese medicinal composition is detected through high performance liquid chromatography; and the conditions of the high performance liquid chromatography are as follows: octadecylsilane bonded silica is adopted as a stationary phase, a mobile phase comprises acetonitrile, methanol and a 0.1% aqueous phosphoric acid solution, gradient elution is carried out for 0-150 min, and the ultraviolet detection wavelength is 230 nm. The relative retention times and the relative peak areas of 43 common peaks in the fingerprint established through the method are calibrated by adopting the absorption peak of protocatechuic acid as a reference peak.

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

The invention discloses a construction method and application of a fingerprint of sweet dream oral liquid. The method comprises the following steps: preparing a test solution, preparing a reference solution, optimizing and determining chromatographic conditions, measuring the fingerprint and processing the obtained fingerprint by using fingerprint software. Chromatographic columns with different stationary phases are connected through a six-way valve; gradient elution is performed by the same mobile phase; and various characteristic index components are well separated. Compared with an existing established fingerprint method, the method achieves that characteristic components of the sweet dream oral liquid can be separated and detected as many as possible, obtains more fingerprint characteristic peaks, and more comprehensively reflects component information of the oral liquid. The method further has the advantages of being easy and convenient to operate, high in precision, good in stability and reproducibility and the like, and the quality of the sweet dream oral liquid can be evaluated more comprehensively and scientifically, so that the quality and the curative effect of the product are guaranteed, and reference is provided for perfection of the quality standard of the product.

The invention discloses an establishment method of inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints specifically comprises the steps of preparing inducing callus cultured Allium sativum L. bulbs into solution for test products through steps such as solid-phase extraction, and performing high-performance chromatographic analysis and detection under certain conditions to obtain HPLC fingerprints of inducing callus cultured Allium sativum L. enzymatic hydrolysate. By analyzing the HPLC fingerprints of 10 batches of samples, characteristic common peaks are determined and standard fingerprints are obtained. The standard fingerprints can provide a reliable basis for variety identification and production quality control of inducing callus cultured Allium sativum L. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints has the advantages that the operation is convenient, the stability is high, the specificity is strong, the repeatability is good, the characteristic peaks are more, the analysis efficiency is high, the truth identification of the inducing callus cultured Allium sativum L. can be realized by adopting the invention, and the interior product quality can be comprehensively, accurately and effectively evaluated.

The invention relates to a method for rapidly identifying traditional Chinese medicine formula particles employing a Fourier transform infrared spectrum, and belongs to the technical field of detection of traditional Chinese medicines. The method comprises the following steps: carrying out absolute ethyl alcohol extraction and ultrasonic extraction on traditional Chinese medicine formula particle samples, and filtering to obtain a sample solution; dropwise adding the sample solution to an agate mortar containing potassium bromide powder; stirring and drying at constant temperature, tabletting and preparing samples; measuring an infrared fingerprint by virtue of a Fourier transform infrared spectrometer; rapidly identifying the traditional Chinese medicine formula particles. According to the method, the defect of poor specificity under a conventional infrared tablet sample preparation method is overcome; rapid detection can be carried out; the result can be compared by virtue of a standard map or can be compared and identified by software similarity. The sample detected by an infrared fingerprint technology is an alcohol extraction concentrated liquor of formula particles; qualitative and semi-quantitative detection can be achieved; the method is easy, accurate, economical, good in repeatability, high in information content and high in spectral characteristics.