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Method for detecting hooker winghead root medicinal materials

A detection method and technology of herbal materials, applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve problems such as lack of unified standards and complex components, and achieve good stability and reproducibility, high detection efficiency, and similarity high effect

Inactive Publication Date: 2014-05-14
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the herb of Pteroptera, due to its complex composition, some specific components cannot truly reflect the quality of the medicinal material, and there is no unified standard for comprehensive research on fingerprints to control its quality

Method used

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  • Method for detecting hooker winghead root medicinal materials
  • Method for detecting hooker winghead root medicinal materials
  • Method for detecting hooker winghead root medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: UFLC detection method of the Tibetan medicine Pterostellaris (external standard method)

[0030] (1) Preparation of the test solution: Take 0.5g of Pterostella herb powder, weigh it accurately, place it in a 100mL conical flask, add 50mL of 70% methanol solution, weigh it, and ultrasonically treat it (power: 200W, frequency : 40kHz) for 30min, put it to room temperature, weigh again, make up the lost weight with 70% methanol solution, filter through a 0.22μm microporous membrane, and take the subsequent filtrate to obtain the final product.

[0031] (2) Preparation of the reference substance solution: Preparation of the reference substance solution: Accurately weigh 10 mg of the reference substance of chlorogenic acid, loganin, swertiroside, rutaecarina, and bissenside A, add methanol to dissolve and determine Volume to 25mL, as the reference substance stock solution. Precisely draw an appropriate amount of the above-mentioned reference substance stock solut...

Embodiment 2

[0035] Example 2: UFLC detection method of Tibetan medicine pterygium (fingerprint comparison method of reference substance)

[0036] (1) Preparation of the test solution: Take 0.5g of Pterostella herb powder, weigh it accurately, place it in a 100mL conical flask, add 50mL of 70% methanol solution, weigh it, and ultrasonically treat it (power: 200W, frequency : 40kHz) for 30min, put it to room temperature, weigh again, make up the lost weight with 70% methanol solution, filter through a 0.22μm microporous membrane, and take the subsequent filtrate to obtain the final product.

[0037] (2) Preparation of the reference substance solution: Preparation of the reference substance solution: Accurately weigh 10 mg of the reference substance of chlorogenic acid, loganin, swertiroside, rutaecarina, and bissenside A, add methanol to dissolve and determine Volume to 25mL, as the reference substance stock solution. Precisely draw an appropriate amount of the above-mentioned reference su...

Embodiment 3

[0041] Example 3: UFLC detection method of Tibetan medicine pterygium (standard fingerprint comparison method)

[0042] (1) Preparation of the test solution: Take 0.5g of Pterostella herb powder, weigh it accurately, place it in a 100mL conical flask, add 50mL of 70% methanol solution, weigh it, and ultrasonically treat it (power: 200W, frequency : 40kHz) for 30min, put it to room temperature, weigh again, make up the lost weight with 70% methanol solution, filter through a 0.22μm microporous membrane, and take the subsequent filtrate to obtain the final product.

[0043] (2) Accurately draw 4 μL of the test product solution, inject it into the chromatograph, and perform detection under the following chromatographic conditions, and then compare the obtained fingerprint of the test product with figure 2 By comparing the standard fingerprints shown, and calculating the similarity, the detection of the Pterosphataceae medicinal material to be tested can be realized. If the simil...

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Abstract

The invention provides a method for detecting hooker winghead root medicinal materials. According to the method, measurement is performed through UFLC (Ultra Fast Liquid Chromatography). According to the UFLC chromatographic detection method, chemical components in hooker winghead root can be effectively separated, the obtained chromatogram map contains multiple characteristic peaks, the similarity of fingerprint spectrums of medicinal materials in various batches is high, and the degree of separation, precision, stability and reproducibility are high. Moreover, the chromatographic detection time is within 20 minutes, the detection efficiency is high, and a new method is provided for detecting the quality of the hooker winghead root medicinal materials.

Description

technical field [0001] The invention relates to a method for detecting the herba pterygium medicinal material. Background technique [0002] Pterocephalus hookeri is Pterocephalus hookeri (C.B.Clarke) The dried whole herb is a traditional Tibetan medicinal material. It has the effects of detoxifying and eliminating plague, clearing heat and stopping dysentery, dispelling wind and dredging numbness, and can treat true brucellosis and blast poison; modern chemical and pharmacological studies have shown that pterygium contains oleanane-type pentacyclic triterpene saponins, cyclic Compounds such as iridoid glycosides, alkaloids, polysaccharides, etc. Among them, iridoid glycosides and oleanane-type pentacyclic triterpene saponins are their therapeutic agents for rheumatoid arthritis (equivalent to Tibetan medicine "true cloth disease") Main active ingredient. [0003] The fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum of a certain type or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张艺赖先荣高燕
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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