HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, construction method and application thereof

A technology of broken-wall decoction pieces and fingerprints, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of short time consumption, comprehensive characteristic peak information, and many characteristic peaks

Active Publication Date: 2019-04-16
ZHONGSHAN ZHONGZHI PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing methods for quality detection of broken-walled decoction pieces of hawthorn, and to provide an HPLC fingerprint of the broken-walled decoction pieces of hawthorn and its construction method and application

Method used

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  • HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, construction method and application thereof
  • HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, construction method and application thereof
  • HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 The construction method of the HPLC fingerprint of hawthorn broken wall decoction pieces

[0065] A method for constructing the HPLC fingerprint of hawthorn broken-wall decoction pieces, comprising the following steps:

[0066] S1. Preparation of the test solution: Precisely weigh the broken pieces of hawthorn, add 70% methanol according to the mass volume ratio of 1:25, infiltrate and homogenate at 12,000 rpm for 2 minutes, take it out and cool to room temperature, and make up with 70% methanol Lose weight, shake well, filter through a 0.22 μm microporous membrane after coarse filtration, and take the filtrate to obtain the test solution;

[0067] S2. Preparation of reference substance solution: take chlorogenic acid as reference substance, add methanol to prepare reference substance solution;

[0068] Chromatographic conditions for S3.HPLC analysis: pass the test solution obtained in S1 through an octadecylsilane bonded silica gel column, the column tempera...

Embodiment 2

[0072] Example 2 Establishment of shared pattern of fingerprints of hawthorn broken-wall decoction pieces

[0073] 1. Identification of reference peaks

[0074] According to the construction method of Example 1, measure the HPLC fingerprint of reference substance solution chlorogenic acid, record the chromatogram, and compare it with the HPLC fingerprint of hawthorn broken wall decoction pieces, analyze the retention time and the ultraviolet absorption situation of the corresponding peak, Such as figure 1 shown.

[0075] Point out: No. 4 peak is chlorogenic acid. Among them, peak No. 6 has moderate height, good resolution and moderate retention time, and was selected as the reference peak.

[0076] 2. Establishment of fingerprints and confirmation of common peaks

[0077] Take 10 batches of hawthorn broken wall decoction pieces (batch numbers are UGP20181201, UGP20181202, UGP20181203, UGP20181204, UGP20181205, UGP20181206, UGP20181207, UGP20181208, UGP20181209, UGP20181210...

Embodiment 3

[0083] Embodiment 3 methodological verification

[0084] 1. Specific inspection

[0085] Get the hawthorn broken wall decoction piece that batch number is UGP20180505, prepare need testing solution and blank need testing solution according to the method for embodiment 1 step S1, sample injection, measure according to the chromatographic condition of embodiment 1 step S3, record chromatogram, as Figure 4 shown. The results show that the solvent has basically no absorption under the chromatographic conditions, and has little influence on the chromatogram of the sample. It shows that the specificity of the established method is good.

[0086] 2. Instrument precision

[0087]Get the hawthorn broken wall decoction piece that batch number is UGP20180505, prepare need testing solution according to the method for embodiment 1 step S1, continuous sample injection 6 times, measure according to the chromatographic condition of embodiment 1 step S3, record chromatogram (see Figure 5...

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Abstract

The invention relates to the Chinese medicine preparation quality control technology field and especially discloses the HPLC fingerprint map spectrum of hawthorn broken wall decoction pieces, a construction method and an application thereof. The construction method comprises the steps of the preparation of the solution of a testing product, comparison product solution preparation, the determination of an HPLC analytical chromatographic condition, the measurement of the fingerprint map spectrum and the like. Through comparing the HPLC fingerprint map spectrums of 10 batches of hawthorn broken wall decoction piece samples, a common peak is determined, and a standard HPLC fingerprint map spectrum is obtained. The construction method has the advantages of simple operation, good reproducibility, a lot of characteristic peaks, and accurate and reliable performance. The HPLC fingerprint map spectrum can comprehensively reflect the characteristic peak information of the sample, and the HPLC fingerprint map spectrum can be used for carrying out quality control on the hawthorn broken wall decoction pieces.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicine preparations, in particular to an HPLC fingerprint of cracked hawthorn decoction pieces, a construction method and application thereof. Background technique [0002] Hawthorn is the dry and mature fruit of Crataegus pimiatificia Bge.var.major N.E.Br. or Crataegus pinnatifida Bge., a plant of the Rosaceae family. Epigastric fullness, dysentery and abdominal pain, amenorrhea due to blood stasis, postpartum stasis, tingling pain in trusted subordinates, chest pain and heartache, hernia pain, hyperlipidemia, and Jiao Hawthorn can enhance the effect of digestion and stagnation. [0003] Traditional Chinese medicine wall-broken decoction pieces use modern ultrafine pulverization technology to break the cell wall of traditional decoction pieces into fine particles with a particle size distribution D90 of less than 45 μm, and then make 30-100 mesh particles. Compar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 甘均龙史军杰彭丽华成金乐陈炜璇陈勇军徐吉银王义娜冯顺
Owner ZHONGSHAN ZHONGZHI PHARMA GRP
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