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Micro-nano composite dual-layer skin framework and manufacturing method thereof

A composite double-layer, micro-nano technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of mechanical strength to be improved, adhesion carrier is not ideal, etc., to achieve low preparation cost, facilitate migration and proliferation, The effect of simple preparation process

Active Publication Date: 2016-11-16
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional bio-3D printing technology, the electrohydrodynamic jet printing technology has higher resolution, and the printed scaffold has finer micron fibers and smaller pore size, which is conducive to the adhesion and growth of cells on the scaffold. , but the prepared scaffold is not ideal as a cell adhesion carrier, and its mechanical strength needs to be improved

Method used

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  • Micro-nano composite dual-layer skin framework and manufacturing method thereof
  • Micro-nano composite dual-layer skin framework and manufacturing method thereof
  • Micro-nano composite dual-layer skin framework and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, see Figure 1~6, a micro-nano composite double-layer skin scaffold, which is composed of a nanofiber layer and a three-dimensional structure scaffold layer. The nanofiber membrane is a flat nanofiber membrane prepared by electrospinning, which is conducive to cell adhesion and growth, the three-dimensional structure support layer is combined on the nanofiber layer, providing a microenvironment for cell growth, which is conducive to cell penetration and proliferation, and the three-dimensional structure support layer is directly written by electrohydrodynamic jet printing method A three-dimensional structural scaffold composed of microfibers, the three-dimensional structural scaffold layer has a higher porosity than the nanofiber layer, and the nanofibrous membrane is used as an epidermal scaffold for the adhesion and growth of skin cells, and the The three-dimensional structure support layer is used as a dermis support for the penetration and proliferat...

Embodiment 2

[0044] This embodiment is basically the same as Embodiment 1, especially in that:

[0045] In this example, see figure 2 and image 3 , a method for preparing a micro-nano composite double-layer skin scaffold, comprising the steps of:

[0046] a. This step is identical with embodiment one;

[0047] b. This step is identical with embodiment one;

[0048] c. This step is identical with embodiment one;

[0049] d. Using an electrohydrodynamic jet printing device, see image 3 , the nanofiber membrane 6 obtained in the step b is fixed on the metal collecting plate 4 of the electrohydrodynamic jet printing device, the metal collecting plate 4 is placed in the ethanol container 5 containing the ethanol solution, and the ethanol liquid is The surface is higher than the metal collecting plate 4 to be 2 mm, and then the container is fixed on the working platform of the electrohydrodynamic jet printer, and as a receiving device, the organic phase B in the step c is loaded into the...

Embodiment 3

[0052] This embodiment is basically the same as the previous embodiment, and the special features are:

[0053] In this example, see figure 2 and image 3 , a method for preparing a micro-nano composite double-layer skin scaffold, comprising the steps of:

[0054] a. This step is identical with embodiment one;

[0055] b. This step is identical with embodiment one;

[0056] c. This step is identical with embodiment one;

[0057] d. Using an electrohydrodynamic jet printing device, see image 3 , the nanofiber membrane 6 obtained in the step b is fixed on the metal collecting plate 4 of the electrohydrodynamic jet printing device, the metal collecting plate 4 is placed in the ethanol container 5 containing the ethanol solution, and the ethanol liquid is The surface is higher than the metal collecting plate 4 to be 2 mm, and then the container is fixed on the working platform of the electrohydrodynamic jet printer, and as a receiving device, the organic phase B in the step...

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Abstract

The invention discloses a micro-nano composite dual-layer skin framework and a manufacturing method thereof which are applied to the skin regenerative tissue engineering. The manufacturing method includes the steps of firstly, preparing a nanofiber membrane layer by the electrospinning technique and utilizing the same as a surface layer of skin; secondly, preparing a three-dimensional framework by direct writing on the nanofiber membrane layer by an electrohydrodynamic inkjet printing method and utilizing the same as dermis. Specifically, the nanofiber membrane refers to a layer of nanofibers uniform in diameter; the nanofibers provide suitable surface morphology for cell adhesion and growth, thereby facilitating cell adhesion and growth; the three-dimensional framework refers to a three-dimension space composed of nanofibers by the electrohydrodynamic inkjet printing method, provides a microenvironment for growth of cells, and facilitates cell infiltration and proliferation; both the nanofiber membrane and the three-dimensional framework are made from biodegradable high-polymer material. Compared with the conventional artificial skin framework, the micro-nano composite dual-layer skin framework has the advantages of high plasticity, good mechanical property, three-dimension structure with high porosity, and high biocompatibility.

Description

technical field [0001] The present invention relates to a skin scaffold imitating extracellular matrix and its preparation method, and also relates to an electrospinning method, a biological 3D printing method and regenerative medicine engineering, in particular to a skin scaffold with a three-dimensional structure and its preparation The method is applied in the technical field of tissue engineering. Background technique [0002] At present, human skin tissue injuries such as large-scale burns are treated by autologous or allogeneic skin transplantation, which not only leads to limited sources of treatment materials, but also causes secondary harm to patients. In recent years, with the rise of bio-3D printing technology, it has become a good choice to use tissue engineering technology to construct skin scaffolds that mimic extracellular matrix to treat skin injuries. [0003] At present, in the field of artificial tissue engineering skin scaffolds, the materials used for t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/40B33Y10/00
CPCA61L27/18A61L27/60B33Y10/00C08L67/04
Inventor 胡庆夕李冬冬刘丽军江晨刘亦李帅刘媛媛
Owner SHANGHAI UNIV
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