Method for constructing refined coronary tablet specific chromatogram and measuring six components

Abstract

The invention relates to a method for constructing a refined coronary tablet specific chromatogram and measuring six components.Firstly, paeoniflorin, rosmarinic acid, salvianolic acid B, ligustilide, cryptotanshinone and a tanshinone IIA reference substance are acquired, 13 batches of refined coronary tablet samples are acquired, and four negative samples which are prepared according to the prescription proportion and process recorded in the Chinese Pharmacopoeia and lack the root of red-rooted salvia, the root of common peony, chuanxiong rhizome and flos carthami respectively are acquired; a reference substance solution, a test article solution and a negative sample solution are prepared in sequence, 0.5 microliter of each solution is precisely absorbed and injected into a liquid chromatograph for measurement so as to obtain a chromatogram, and calculation is conducted with a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system to obtain the specific chromatogram.The specific chromatogram constructed for the first time contains comprehensive characteristic peaks, positioning is accurate, the authenticity of the Chinese patent medicine is effectively identified, the quality of the Chinese patent medicine is effectively evaluated, multiple components of the refined coronary tablets can also be detected quickly, accurately and quantitatively, and the method is expected to become a standard method for quality control of the Chinese patent medicine refined coronary tablets.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to the construction of a characteristic map of refined Guanxin Tablets and a method for determining six components. Background technique [0002] Refined Guanxin Tablets is a traditional Chinese medicine preparation composed of five Chinese herbal medicines, Danshen, Radix Paeoniae Rubra, Chuanxiong, Safflower, and Jiangxiang. The chemical components are very complex. The preparation has the effect of promoting blood circulation and removing blood stasis. It is used for chest obstruction caused by internal stagnation of blood stasis, symptoms include chest tightness and tingling pain in the precordial area; coronary heart disease angina pectoris sees the above syndromes. Its preparation method is to extract the volatile oil from the balsamic, the distilled aqueous solution is collected in another device, and the other four flavors of Chinese herbal ...

AI Technical Summary

Problems solved by technology

Zhang Lingyan et al. (Chinese herbal medicine. 46 (14): 2092-2095) measured the content of 7 kinds of index components in refined Guanxin Tablets. They can only measure the contents of 7 kinds of qualitative components such as Danshensu, which cannot be used for refining Guanxin Tablets. Unable to detect qualitative components, the quality controllability of refined Guanxin tablets is poor, and the quality of refined Guanxin tablets cannot be comprehensively and effectively measured

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