HPLC fingerprint detection method for liver-comforting decoction

A technology of fingerprint and detection method, which is applied in the field of quality detection of drugs, can solve problems such as the influence of consistency between batches of quality, instability of medicinal materials, unfavorable curative effect, etc., and achieve quality uniformity, many characteristic peaks, and simple detection methods Effect

Active Publication Date: 2019-10-11
HENAN TALOPH PHARMACEUTICAL STOCK CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Huagan decoction has a long and rich history of human use in my country, but due to the instability of its medicinal materials and complex ingredients, the consistency of its quality between batches is easily affected, which is not conducive to the steady development of curative effect

Method used

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  • HPLC fingerprint detection method for liver-comforting decoction
  • HPLC fingerprint detection method for liver-comforting decoction
  • HPLC fingerprint detection method for liver-comforting decoction

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Experimental program
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Embodiment 1

[0029] 1 material

[0030] 1.1 Instruments and reagents:

[0031] Instruments: Ultimate 3000 High Performance Liquid Chromatography (Thermo Fisher, Germany); DAD-3000 Diode Array Detector (Thermo Fisher, Germany); MS105DU Electronic Analytical Balance (Mettler-Toledo); KQ-300E Ultrasonic Cleaner Machine (Kunshan Ultrasonic Instrument Co., Ltd.); HH-S26S digital display constant temperature water bath, etc.

[0032] Reagents: acetonitrile, chromatographically pure (Merck); formic acid, chromatographically pure (Fisher); phosphoric acid, chromatographically pure (Kermel); ethanol, analytically pure (Kermel); Watsons purified water.

[0033] Paeoniflorin reference substance (batch number: 110736-201842, China National Institutes for Food and Drug Control);

[0034] Huagan decoction was provided by Henan Tailong Pharmaceutical Co., Ltd., and the batch number information is shown in Table 1.

[0035] Table 1 Batch number information of Huagan decoction

[0036]

[0037]

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Abstract

The invention discloses an HPLC fingerprint detection method for liver-comforting decoction. According to the method, a high performance liquid chromatography under a specific condition is adopted tocarry out detection so as to obtain a fingerprint with 8 common peaks, so that the multi-component information of the products can be reflected. The method is simple, good in reproducibility and multiple in characteristic peaks, is capable of ensuring the quality uniformity and stability of the products and providing scientific basis for the quality control of the products.

Description

technical field [0001] The invention relates to a method for detecting the quality of medicines, in particular to a method for detecting HPLC fingerprints of Huaganjian. Background technique [0002] Huaganjian is an ancient classic recipe, which comes from Volume 51 of "Jingyue Quanshu" by Zhang Jingyue in the Ming Dynasty, Hanzheng. The original text of the prescription is: cure anger and damage the liver, so the qi is reversed and the fire is caused, resulting in syndromes such as dysphoria, hypochondriac pain, fullness and blood circulation. Qingpi and tangerine peel are 2 qian each, peony 2 qian, peony bark, gardenia (stir-fried), and Alisma alisma are 2 qian each, and Tu Fritillaria 2 and 3 qian. One and a half cups of water, fry for seven or eight minutes. Food away from warm clothes. [0003] This prescription has the functions of soothing the liver and relieving depression, clearing the liver and purging fire. It is used to treat anger that injures the liver, rev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36G01N2030/047
Inventor 郭中华张立壮陈杰中其木格张志平刘伟伟胡宇
Owner HENAN TALOPH PHARMACEUTICAL STOCK CO LTD
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