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Establishing method and use of flue-cured tobacco GC/MS fingerprint

A technology of fingerprint spectrum and establishment method, which is applied in the field of quality analysis of flue-cured tobacco, brand identification of flue-cured tobacco and threshing formula, can solve the problems such as no patent application in the application field, and achieve the improvement of production level, economic benefit and baseline separation. Good effect with many characteristic peaks

Active Publication Date: 2012-06-13
YUNNAN TOBACCO LEAF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore the method that this patent adopts and field of application have not yet seen patent application

Method used

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  • Establishing method and use of flue-cured tobacco GC/MS fingerprint
  • Establishing method and use of flue-cured tobacco GC/MS fingerprint
  • Establishing method and use of flue-cured tobacco GC/MS fingerprint

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Experimental program
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Effect test

Embodiment 1

[0031] The method for establishing a GC / MS fingerprint of flue-cured tobacco according to the present invention is realized through the following steps:

[0032] 1) Preparation of the solution to be tested: Connect a 500mL flat-bottomed flask to one end of the distillation extraction device. Fill the flat-bottomed flask with 20.00g tobacco powder, and add 350mL pure water and 0.3mL phenethyl propionate with a concentration of 4μL / mL. And use a voltage-controllable electric heating mantle for heating; connect a 100mL flask to the other end of the distillation extraction device, put 50mL dichloromethane in the flask, place the flask on a water bath and heat, and distill at a temperature of 56°C. Extract for 2.5 hours, remove a 100mL flask, add 5g of anhydrous sodium sulfate to it, seal it overnight, put it on a rotary evaporator and concentrate to 1mL, pass through a 0.45μm filter membrane, the filtrate is the solution to be tested;

[0033] 2) Obtaining GC / MS fingerprints of flue-cu...

Embodiment 2

[0037] The tested tobacco variety K326, grade C3F, was taken from 15 different tobacco producing areas in Yunnan Province. All tobacco samples were collected in 2009 and uniformly redryed into shredded tobacco. The tobacco sample is dried at 40°C, crushed with a plant grinder, passed through a 60-mesh sieve, sealed with a plastic bag, and stored in an ultra-low temperature refrigerator (-80°C) for testing. The determination method is as follows:

[0038] 1) Preparation of the solution to be tested: Connect a 500mL flat-bottomed flask to one end of the distillation extraction device, and fill the flat-bottomed flask with 20.00g tobacco powder, and add 450mL pure water and 0.6mL phenethyl propionate with a concentration of 4μL / mL. And heat the flat-bottomed flask with an electric heating mantle with controllable voltage; connect a 100mL flask to the other end of the distillation extraction device, put 80mL dichloromethane in the flask, place the flask on a water bath and heat it at...

Embodiment 3

[0043] The tested tobacco variety K326, grade B2F, was taken from 17 different tobacco producing areas in Yunnan Province. According to the preparation method and analysis method of Example 1, the cluster diagram of different samples was obtained ( Figure 4 ).

[0044] Cluster analysis Figure 4 It can be seen that when the distance is about 0.05, the B2F grade tobacco leaves in Yunnan Province in 2009 can be divided into 6 categories: the first category is Shizong, Luoping, and Honghe; the second category is Pu'er, Wenshan, and Lu Liang and Lincang; the third category is Dali, Zhaotong, Baoshan; the fourth category is Xundian, Chuxiong, Fuyuan, Zhanyi; the fifth category is Qilin, Yuxi; and Fuyuan is the sixth category.

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Abstract

The invention provides an establishing method and a use of a flue-cured tobacco GC / MS fingerprint. The establishing method of the flue-cured tobacco GC / MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC / MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC / MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC / MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC / MS detection technology so that the flue-cured tobacco GC / MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC / MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

Description

technical field [0001] The invention belongs to the field of tobacco quality classification, and in particular relates to a quality analysis method in flue-cured tobacco quality analysis, flue-cured tobacco brand identification and threshing formula. technical background [0002] Tobacco, as a natural plant, has quite complex components. Currently, there are more than 5,000 aroma components that have been isolated and identified. These components have a greater or lesser impact on its quality. It is obviously biased to judge the quality of tobacco if only the main chemical components are analyzed based on conventional chemical testing. Therefore, the identification of tobacco quality mainly relies on manual smoking evaluation. Although artificial evaluation can be grasped as a whole, it is easily affected by subjective factors such as personal emotions and sensory differences. Sometimes there are large deviations, and the workload is heavy. [0003] Fingerprint technology...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 矣跃平袁仕信何云燕刘磊尹晓东曹林海涂书新何结望阎铁军李琳
Owner YUNNAN TOBACCO LEAF
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