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Establishment method and standard spectrum of hplc fingerprint of Shouhui Tongbian Capsules

A technology of laxative and fingerprinting, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems that have not yet been published in patents and literature reports, and achieve uniformity and stability, good reproducibility, and characteristic The effect of many peaks

Active Publication Date: 2020-03-10
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the quality of Shouhui Laxative Capsules is controlled by fingerprints, and there are no patent publications and literature reports at home and abroad.

Method used

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  • Establishment method and standard spectrum of hplc fingerprint of Shouhui Tongbian Capsules
  • Establishment method and standard spectrum of hplc fingerprint of Shouhui Tongbian Capsules
  • Establishment method and standard spectrum of hplc fingerprint of Shouhui Tongbian Capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Establishment of HPLC Standard Fingerprint of Shouhui Tongbian Capsules

[0025] 1 Instruments and reagents

[0026] 1.1 Instrument

[0027] Agilent 1100 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0028] 1.2 Reagent

[0029] Shouhui Tongbian Capsules were provided by Lunan Houpu Pharmaceutical Co., Ltd., see Table 1; acetonitrile was chromatographically pure, water was double distilled water, and the rest of the reagents were analytically pure.

[0030] Table 1 Batch No. of Test Samples of Shouhui Laxative Capsules

[0031]

[0032] 2 Methods and results

[0033] 2.1 Chromatographic conditions: Chromatographic column: Tianjin Turner Kromasil C 18 (4.6 × 250mm, 5um) column; mobile phase: acetonitrile is used as mobile phase A, and 0.10% phosphoric acid aqueous solution is used as mobile phase B in volume percentage, and the gradient elution is carried o...

Embodiment 2

[0053] Example 2 Establishment of HPLC Standard Fingerprint of Shouhui Tongbian Capsules

[0054] 1 Instruments and reagents

[0055] 1.1 Instrument

[0056] Agilent 1100 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0057] 1.2 Reagent

[0058] Shouhui Tongbian Capsules were provided by Lunan Houpu Pharmaceutical Co., Ltd., see Table 4; acetonitrile was chromatographically pure, water was double distilled water, and other reagents were analytically pure.

[0059] Table 4 Batch No. of Test Samples of Shouhui Laxative Capsules

[0060]

[0061] 2 Methods and results

[0062] 2.1 Chromatographic conditions: Chromatographic column: Tianjin Turner Kromasil C 18 (4.6 × 250mm, 5um) column; mobile phase: acetonitrile is used as mobile phase A, and 0.05% phosphoric acid aqueous solution is used as mobile phase B in volume percentage, and the gradient elution is carried out in the ...

Embodiment 3

[0068] Example 3 Establishment of HPLC Standard Fingerprint of Shouhui Laxative Capsules

[0069] 1 Instruments and reagents

[0070] 1.1 Instrument

[0071] Agilent 1100 high performance liquid chromatography (USA): DAD detector, quaternary low-pressure gradient pump, AgilentOpen Lab chromatography workstation.

[0072] 1.2 Reagent

[0073] Shouhui Tongbian Capsules were provided by Lunan Houpu Pharmaceutical Co., Ltd., see Table 5; acetonitrile was chromatographically pure, water was double distilled water, and the rest of the reagents were analytically pure.

[0074] Table 5 Batch No. of Test Samples of Shouhui Laxative Capsules

[0075]

[0076] 2 Methods and results

[0077] 2.1 Chromatographic conditions: Chromatographic column: Tianjin Turner Kromasil C 18 (4.6 × 250mm, 5um) column; mobile phase: acetonitrile is used as mobile phase A, and 0.20% phosphoric acid aqueous solution is used as mobile phase B in volume percentage, and the gradient elution is carried o...

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Abstract

The invention discloses a polygonum multiflorum and aloe purgation capsule HPLC fingerprint spectrum establishing method. The method comprises the steps of preparation of a solution for testing products, determination of the HPLC chromatographic condition and HPLC standard fingerprint spectrum making. A polygonum multiflorum and aloe purgation capsule HPLC fingerprint spectrum obtained through themethod has 25 common peaks, and the relative retention time tR thereof is 0.101, 0.108, 0.137, 0.268, 0.282, 0.312, 0.690, 0.717, 0.798, 0.887, 0.925, 1.000, 1.052, 1.125, 1.173, 1.255, 1.270, 1.409,1.489, 1.520, 1.648, 1.861, 1.893, 2.048 and 2.158 separately in sequence. The spectrum obtained through the fingerprint spectrum establishing method has many characteristic peaks, the reproducibility is good, operation is easy, and the stability is high; by means of comparison of the standard fingerprint spectrum common peaks, the quality of polygonum multiflorum and aloe purgation capsules canbe comprehensively evaluated and controlled, and the uniformity and stability of the inner quality are ensured.

Description

technical field [0001] The invention relates to a method for establishing an HPLC fingerprint of Shouhui Laxative Capsules and a standard fingerprint thereof, belonging to the field of analysis of traditional Chinese medicine preparations. Background technique [0002] Chinese patent CN100453105C discloses a traditional Chinese medicine composition with laxative detoxification, weight loss and fat-lowering functions and a preparation method. The product is a Chinese medicine compound preparation "Shouhui Laxative Capsule" for treating functional constipation. The prescription of Shouhui Tongbian Capsules is guided by the theory of traditional Chinese medicine and integrated with clinical experience. It is supplemented with products for nourishing blood and moistening dryness, and products for nourishing qi and invigorating the spleen in order to reduce turbidity and eliminate turbidity. The taste of the medicine of ruffian achieves both tonifying and reducing. It is used for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 刘武占关永霞
Owner LUNAN PHARMA GROUP CORPORATION
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