Establishment method of hplc-elsd fingerprint of Anemarrhena medicinal material

Abstract

The invention relates to an establishing method of rhizoma anemarrhenae HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) fingerprint and a standard fingerprint established by using the establishing method. The establishing method comprises chromatographic conditions, the preparation of a test substance solution, and the preparation of a control substance solution, wherein the chromatographic condition is a Thermo C8 chromatographic column (4.6mm*250mm, 5 microns); a mobile phase is an acetonitrile-0.2% acetic acid aqueous solution for gradient elution; the column temperature is 20 DEG C; the flow speed is 1mL.min<-1>; the flow speed of an ELSD atomizer is 2.6L.min<-1>; the temperature of a drift tube is 100 DEG C; and the sampling size is 20 microlitres. The establishing method provided by the invention has the advantages of good stability and repeatability, high precision, capability of simultaneous reflection of the characteristics of saponins and xanthones, which are the main chemical ingredients of the rhizoma anemarrhenae, and capability of reflecting the quality of the rhizoma anemarrhenae according to the overall characteristics of the chromatogram; and the establishing method can be used as one method for controlling the quality of the rhizoma anemarrhenae.

Description

technical field [0001] The invention relates to a method for establishing a fingerprint of an anemarrhena medicinal material and a standard fingerprint thereof, in particular to a method for establishing an HPLC-ELSD fingerprint of an anemarrhena medicinal material and a standard fingerprint thereof. Background technique [0002] Anemarrhena asphodeloides Bunge. is a commonly used Chinese medicinal material. It comes from the dried rhizome of Anemarrhena asphodeloides Bunge. In my country, it is mainly distributed in Hebei, Shanxi, Inner Mongolia, Liaoning, Heilongjiang, Jilin and other provinces, and also in Henan, Shandong and Anhui provinces. [0003] Over the past 10 years, scholars at home and abroad have conducted extensive and in-depth research on the chemical components of Anemarrhena, and found that Anemarrhena contains steroidal saponins, bilipidones, lignans, polysaccharides, organic acids and Trace element components, the main components of which are steroidal s...

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Problems solved by technology

[0016] The purpose of the present invention is to solve the problem that the overall quality of Anemarrhena medicinal material cannot be comprehensively and comprehensively reflected and controlled in the current quality evaluation and component analysis of Anemarrhena medicinal material, thereby providing an HPLC-ELSD fingerprint analysis method for Anemarrhena medicinal material, including chromatographic conditions , the preparation of the test solution, the preparation of the reference solution

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