Detection method for stevioside in wine

Abstract

The invention relates to a detection method for stevioside in wine. The detection method comprises the following steps that: 1) weighing 8-12ml of wine test sample in a centrifuge tube, adding 25-35mlof extraction liquid, and carrying out vortex oscillation, ultrasonic extraction and filtering for standby; 2) continuously loading the filtered extraction liquid to a silica gel solid phase extraction small column at a speed of 1-3ml / min, and drying by nitrogen to enable the wine test sample to be enriched on the silica gel solid phase extraction small column; and 3) carrying out elution on theenriched silica gel solid phase extraction small column by elution liquid, collecting the elution liquid subjected to elution, after the elution liquid is dried and concentrated, carrying out volume metering by first solution to obtain sample solution, and after filtering is carried out, loading the sample liquid to a high performance liquid chromatograph for detecting the contents of the stevioside in sample liquid. By use of the detection method, through extraction, elution and detection, the contents of the stevioside in the wine can be quickly detected, detection time is obviously shortened, and the detection method is free from the interference of multiple components in a matrix, is simple in operation, is accurate in detection results and is suitable for quickly detecting the stevioside in the wine.

Description

technical field [0001] The invention relates to the field of food additive detection, in particular to a method for detecting steviol glycosides in wine. Background technique [0002] Steviol glycoside is a mixture of diterpene glycosides containing 8 components extracted from the leaves of the Compositae herb Stevia rebaudiana, also known as stevioside and stevioside. In addition to its high sweetness and low calorie value, stevioside In addition to its properties, it also has many functional activities, such as lowering blood sugar, lowering blood pressure, anti-tumor, antidiarrheal, etc. This new type of sweetener is widely used in different fields such as food, beverage, medicine, and daily chemical industry. Although stevioside has many advantages, it is also controversial. In recent years, it has been reported that it is carcinogenic, but many experiments have proved that stevioside is still very safe when used reasonably. [0003] Wine is known as "the oldest drink a...

AI Technical Summary

Problems solved by technology

People's consumption concept is changing, and it is developing towards low-alcohol and healthy, and the low-alcohol wine has the functions of softening brain capillaries and beautifying, which can meet consumers' health requirements, coupled with the popularization of wine knowledge and the promotion of new media. In the future, China's wine consumption will increase rapidly, and the development of wine in China has great potential. However, sweeteners, such as sweet glycosides, are added to wine. If the content exceeds the standard, it will pose a potential threat to human health. Therefore, it is necessary to detect The content of additives in wine, existing detection methods include spectrophotometry, capillary electrophoresis, gas chromatography, etc., these methods are due to the multi-component interference in the matrix, the error is large, and the pretreatment method is loaded down with trivial details, low efficiency, can not Rapid detection of steviol glycoside content in wine