Quality control method for red sage root and notoginseng preparation

A technology of Sanqi and Danshen, which is applied in the field of HPLC-DAD fingerprint identification of Danshen and Sanqi compound, which can solve the problems of failure to identify at the same time

Inactive Publication Date: 2006-07-26
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology fails to simultaneously identify multiple types of active ingredients in Danshen and Panax notoginseng with fingerprints

Method used

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  • Quality control method for red sage root and notoginseng preparation
  • Quality control method for red sage root and notoginseng preparation
  • Quality control method for red sage root and notoginseng preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] 1 Experimental materials

[0087] 1.1 Instruments and equipment:

[0088] Agilent 1100 series high performance liquid chromatography, including G1312A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315A DAD detector, HP Chemstation chromatography workstation (U.S. HP Company); rotary thin film evaporator (Switzerland) Büchi company).

[0089] 1.2 Reagents and reagents:

[0090] Medicinal materials: Danshen (Shaanxi Tasly Plant Pharmaceutical Co., Ltd., Shangluo, Shaanxi), identified by Professor Li Ping as the dry root of Salvia miltiorrhiza Bge. The dry root of Panax notoginseng (Burk.) F.H.Chen.

[0091] 2. Experimental method

[0092] 2.1 Chromatographic conditions

[0093]Chromatographic column: C18 chromatographic column (ZORBAX ODS 4.6×250mm ID, 5μm) and C18 pre-column (4.6×12.5mmID, 5μm); column temperature: 30°C; mobile phase, A is 0.1% phosphoric acid water; B is acetonitrile; A+B=100%, gradient elution: 0-10min, 7-17%B, 10-12mi...

Embodiment 2

[0098] Take 20 commercially available Danqi Tablets (Hunan Anbang Pharmaceutical Co., Ltd., batch number: 040803), remove the sugar coating and grind them into powder, accurately weigh 0.5g, put in a 25ml brown volumetric flask, and dilute to volume with 70% methanol. Ultrasonic extraction was performed for 30 min, allowed to cool, and 70% methanol was added to make up the weight. The solution was passed through a 0.45 μm membrane filter. Other conditions are with embodiment 1. Inject 10 μl for chromatographic analysis, get the HPLC-DAD fingerprint of seven tablets of Dandan, see Image 6 .

Embodiment 3

[0100] Get commercially available Compound Danshen Tablets (Guangdong Baiyunshan Pharmaceutical Factory, batch number: 03121024; Nanjing Tongrentang Pharmaceutical Co., Ltd., batch number: 040602; Zhejiang Huqing Yutang Pharmaceutical Co., Ltd., batch number: 050308) 20 tablets, ground into powder, and accurately weighed Take 0.5g, put it in a 25ml brown volumetric flask, and dilute to volume with 70% methanol. Ultrasonic extraction was performed for 30 min, allowed to cool, and 70% methanol was added to make up the weight. The solution was passed through a 0.45 μm membrane filter. Other conditions are with embodiment 1. Inject 10 μl of sample for chromatographic analysis, and obtain the HPLC-DAD fingerprints of Compound Danshen Tablets from 3 manufacturers, see Figure 7-9 .

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Abstract

The invention relates to a HPLC-DAD fingerprint pattern identification method for the compound of salvia miltiorrhizae and pseudo-ginseng, which can be applied as one of the quality control indexes for compound preparations containing salvia miltiorrhizae and pseudo-ginseng medicinal materials such as Danqi tablet, compound salvia miltiorrhizae tablet and compound salvia miltiorrhizae drop pill.

Description

Technical field: [0001] The invention relates to the field of quality control of natural medicines, in particular to the field of fingerprints, in particular to an HPLC-DAD fingerprint identification method of Danshen and Panax notoginseng compound prescriptions. It can be used as one of the indicators for the quality control of compound preparations containing Danshen and Panax notoginseng. Background technique: [0002] Preparations containing Danshen and Panax notoginseng, commonly known as compound Danshen preparations, are widely used clinically to treat cardiovascular diseases. The common ones are Compound Danshen Tablets, Compound Danshen Dripping Pills, Danqi Tablets, Guanxin Danshen Tablets, etc. [0003] Salvia miltiorrhiza and Panax notoginseng are the two main components of compound salvia miltiorrhiza preparations. Salvia miltiorrhiza is the dry root of Salvia miltiorrhiza Bge., a plant in the labiatae family. Tune, amenorrhea, dysmenorrhea, accumulation of lu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61K36/258A61P9/10G01N30/02G01N33/15
Inventor 李萍韦英杰李松林
Owner CHINA PHARM UNIV
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