Method for establishing Anoectochilus roxburghii fingerprint, and Anoectochilus roxburghii fingerprint and Anoectochilus formosanus Hay fingerprint

A technology of fingerprint spectrum and establishment method, which is applied to the fingerprint spectrum of Fujian clematis and Taiwan clematis, and the establishment of clematis fingerprints, can solve the problems of inability to accurately identify and evaluate the quality of clematis medicinal materials, and achieve relative Stable retention time, quality control, and good method precision

Active Publication Date: 2012-11-07
闽王(厦门)药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In terms of quality evaluation of Auranthus clematis, in the literature published at home and abroad, microscopic identification, thin-layer chromatography identification, or determination of single component content, such as flavonoids, auroglutinin, etc., are generally used, and are used as Obviously, this quality evaluation method cannot accurately identify and evaluate the quality of the medicinal materials

Method used

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  • Method for establishing Anoectochilus roxburghii fingerprint, and Anoectochilus roxburghii fingerprint and Anoectochilus formosanus Hay fingerprint
  • Method for establishing Anoectochilus roxburghii fingerprint, and Anoectochilus roxburghii fingerprint and Anoectochilus formosanus Hay fingerprint
  • Method for establishing Anoectochilus roxburghii fingerprint, and Anoectochilus roxburghii fingerprint and Anoectochilus formosanus Hay fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Establish fingerprints of A. roxburghii:

[0074] (1) Take 4 batches of authentic Fujian Clematis clematis tissue culture products to prepare the test solution, specifically: respectively accurately weigh 0.2 g of the fine powder of the above test product and place it in a stoppered Erlenmeyer flask. Add 20mL of methanol, seal it tightly, weigh it accurately, and after ultrasonic treatment for 45 minutes, let it cool to room temperature, make up the lost weight with methanol, shake well, take the supernatant after centrifugation for 10 minutes, and pass the supernatant through 0.25μm Membrane filtration obtains the test solution;

[0075] (2) Chromatographic conditions: chromatographic column is Aglient Extend C18 (4.6×250mm, 5μm); elution method: use gradient eluent composed of 0.4% phosphoric acid and methanol as mobile phase for gradient elution; column temperature: 30°C ;Ultraviolet detection: detection wavelength is 316nm; flow rate: 1.0mL / min; the gradient elutio...

Embodiment 2

[0095] (1) The establishment of the fingerprint of the test product includes the following steps:

[0096] a Preparation of the test solution: take Fujian clematis (pointed leaf) tissue culture product, Fujian clematis (round leaf) tissue culture product, Fujian clematis (big leaf) wild product, Fujian clematis ( Small leaf) wild product, commercially available clematis counterfeit 1, commercially available clematis counterfeit 2, commercially available clematis counterfeit 3, commercially available clematis counterfeit 4 and commercially available variegated leaf orchid for testing The preparation of the solution is specifically: respectively accurately weigh 0.2 g of the fine powder of the above-mentioned test product and place it in a stoppered Erlenmeyer flask, accurately add 20 mL of methanol, seal it tightly, accurately weigh it, and after ultrasonic treatment for 45 minutes, let it cool to room temperature , make up the lost weight with methanol, shake well, take the su...

Embodiment 3

[0105] Establish fingerprints of A. taiwanensis:

[0106] (1) Take 4 batches of authentic Taiwan clematis to prepare the test solution, specifically: respectively accurately weigh 0.2 g of the fine powder of the above test product and place it in a stoppered Erlenmeyer flask, and precisely add 20 mL of methanol , densely packed, accurately weighed, after ultrasonic treatment for 45 minutes, let cool to room temperature, make up for the lost weight with methanol, shake well, take the supernatant after centrifugation for 10 minutes, and filter the supernatant through a 0.25 μm filter membrane , to obtain the test solution;

[0107] (2) Chromatographic conditions: chromatographic column is Aglient Extend C18 (4.6×250mm, 5μm); elution method: use gradient eluent composed of 0.4% phosphoric acid and methanol as mobile phase for gradient elution; column temperature: 30°C ;Ultraviolet detection: detection wavelength is 316nm; flow rate: 1.0mL / min; the gradient elution program is car...

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Abstract

The present invention discloses a method for establishing an Anoectochilus roxburghii fingerprint, and an Anoectochilus roxburghii fingerprint and an Anoectochilus formosanus Hay fingerprint. The method provided by the present invention comprises the following factors: (1) preparation of a test solution; (2) chromatographic condition: using an octadecylsilane bonded silica gel as a column filler; elution mode: using a gradient elution solution consisting of phosphoric acid and methanol as a mobile phase to conduct gradient elution; and ultraviolet detection with a detection wave length of 310-320nm; and (3) determination in accordance with a high performance liquid chromatography to obtain a fingerprint. The method provided by the invention employs high-performance liquid chromatography to establish the Anoectochilus roxburghii fingerprint and improve the level of quality control of Anoectochilus roxburghii; the invention provides an internal control peak fingerprint discrimination method to solve the problem of lack of reference substance for relevant components of Anoectochilus roxburghii; and the internal control peak is used as a reference to obtain stable relative retention time of each characteristic peak and relative peak area, and realize better precision of the method.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and in particular relates to a method for establishing fingerprints of clematis and fingerprints of clematis from Fujian and clematis from Taiwan. Background technique [0002] Clematis is Anoectochilus roburghii (Wall.) Lindl), Anoectochilus formosanus Hay, a rare perennial Chinese herbal medicine. It is used in a wide range of folks, and it is known as "King of Medicine", "Golden Grass", "Miraculous Medicine" and "Bird Ginseng" in Hong Kong, Taiwan and Southeast Asia. Flat in nature and sweet in taste, it belongs to the lung, liver, kidney and bladder channels. It has the effects of clearing heat and cooling blood, detoxifying and reducing swelling, dispelling wind and dampness, calming convulsions and calming liver, nourishing kidney, diuresis, moistening lung and relieving cough. It is used to treat children with convulsions, high fever, rheumatoid arthritis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 胡珊梅陈咏梅吴波
Owner 闽王(厦门)药业有限公司
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