Method for detecting nitrofuran metabolite residues in chicken ossein and chicken bone white soup

A technology of nitrofuran and chicken bone soup, which is applied in the detection of drug residues, pretreatment detection of nitrofuran metabolites in chicken bone and chicken bone soup, and can solve the problems of inability to stratify, large ethyl acetate, and inability to To achieve the extraction effect and other problems, to achieve the effect of improving efficiency, convenient pre-treatment, simple and feasible operation process

Inactive Publication Date: 2020-05-19
山东中质华检测试检验有限公司
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Problems solved by technology

[0005] Except for the above-mentioned matrix, the extraction of nitrofuran metabolites in samples with relatively large oil content and complex matrix, such as chicken bone essence and chicken bone soup, cannot achieve a good extraction effect according to the above standards, and some even because the oil content is too high. So large that it cannot be separated during the extraction process of ethyl acetate

Method used

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  • Method for detecting nitrofuran metabolite residues in chicken ossein and chicken bone white soup
  • Method for detecting nitrofuran metabolite residues in chicken ossein and chicken bone white soup
  • Method for detecting nitrofuran metabolite residues in chicken ossein and chicken bone white soup

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Embodiment

[0051] Take a certain concentration of standard solution for the above treatment, and establish a standard working curve according to the concentration and peak area of ​​the standard solution;

[0052] Described liquid chromatography condition is: C18 chromatographic column, 50mm * 3.0mm id, particle diameter 2.7 μ m; Mobile phase: the ammonium acetate of 0.5mmol / L contains 0.1% formic acid and acetonitrile; Gradient elution (its elution program is as shown in the table 1); flow rate 0.4mL / min; column temperature: 30°C; injection volume: 10μL; detection mode (conditions as shown in Table 2): multiple reaction detection; mode: positive ion mode.

[0053] Table 1 Liquid chromatography elution program

[0054]

[0055] Table 2 Mass Spectrometry Conditions

[0056]

[0057]

[0058]

[0059] Table 3 The recovery rate and deviation of nitrofuran drugs in chicken bone element and chicken bone white soup

[0060]

[0061] Result calculation: the content of nitrofura...

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Abstract

The invention discloses a method for detecting nitrofuran metabolite residues in chicken ossein and chicken bone white soup. The method comprises steps of (1) weighing a sample; (2) adding diatomite for grinding treatment; (3) adding extracting solution into the treated sample, and uniformly mixing; (4) carrying out derivatization treatment; (5) adjusting a pH value to about 7.2-7.5 by using acidand alkali; (6) extracting with ethyl acetate, and collecting extraction solution; and (7) carrying out 40 DEG C nitrogen blow-drying of the extract, and allowing 0.1% formic acid water to pass through a film machine at a constant volume for detection. The detection method of nitrofuran metabolite is simple, and pretreatment is convenient and efficient.

Description

technical field [0001] The invention relates to a detection method for drug residues, in particular to a pretreatment detection method for nitrofuran metabolites in chicken bone essence and chicken bone soup, and belongs to the field of food safety detection. Background technique [0002] Nitrofuran drugs are synthetic broad-spectrum antibacterial drugs with a 5-nitro structure, mainly including furazolidone, furaltadone, nitrofurazone, nitrofurantoin, and nifsol. They act on the microbial enzyme system, inhibit acetyl coenzyme A, interfere with the metabolism of microbial carbohydrates, and thus play an antibacterial role. Because nitrofuran drugs have carcinogenic and teratogenic side effects on the human body, the Ministry of Agriculture of the People's Republic of China proposed in Announcement No. 235 and No. 560 that nitrofuran drugs are prohibited to be used in the breeding process, and should not be used in animal food. Check out. However, it is still used in lives...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/06G01N30/02G01N2030/062
Inventor 王成梅郑文娟仲光凤李淑敏崔浩威
Owner 山东中质华检测试检验有限公司
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