Method for separating and extracting L-ribose

A ribose and arabinose technology, applied in the field of separation of biochemical products, can solve problems such as inability to crystallize products, lower economic and social benefits, and cumbersome process operations, so as to improve product purity and yield, reduce production costs, yield and The effect of high purity

Active Publication Date: 2010-07-21
ANHUI BBCA FERMENTATION TECH ENG RES
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Problems solved by technology

In the obtained L-ribose reaction solution, it contains more unreacted raw material L-arabinose, and impurities such as catalysts, pigments, and by-products such as xylose. Although it still contains a relatively high concentration of L-ribose, it cannot Qualified products are crystallized, which leads to waste of resources and

Method used

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  • Method for separating and extracting L-ribose
  • Method for separating and extracting L-ribose

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1) Take 20L of the reaction solution of L-arabinose to synthesize L-ribose, and carry out continuous centrifugation at a speed of 3000r / min until no more liquid is centrifuged out;

[0030] 2) The solution obtained after centrifugation was chromatographed with D318 macroporous weakly basic anion exchange resin, the resin loading was 42L, and the resin layer height was 1.5m, and eluted with water;

[0031] The obtained Ionic solution: L-ribose content is 11.87%, purity is 37.04%, xylose content is 24.60%, L-arabinose content is 10.32%, dry matter content is 32.05%, dark green.

[0032] 3) Take 26L of the ionizing liquid after the chromatography, start the simulated moving bed sample injection treatment, adjust the water inlet rate to be 51.66ml / min, the sample injection rate to be 11.48ml / min, the waste water outflow rate to be 51.66ml / min, and the product to flow out. The rate was 11.48ml / min, the backfill rate was 40.18ml / min; the resulting 35.58L L-ribose-rich solutio...

Embodiment 2

[0035] 1) Take 26L of the ionizing solution after the chromatography in Example 1, open the simulated moving bed sample injection treatment, adjust the water inlet rate to be 45.92ml / min, the sample injection rate to be 8.61ml / min, and the waste water outflow rate to be 45.92ml / min. min, the product outflow rate was 8.61ml / min, and the backfill rate was 34.44ml / min; the obtained 36.90L L-ribose-rich solution was light yellow, the L-ribose content was 8.28%, the dry matter was 10.19%, and the purity was 81.2% , the recovery rate of L-ribose was 99%;

[0036] 2) Concentrating and crystallizing the solution rich in L-ribose to obtain L-ribose, and the crystal purity of L-ribose is increased to 99.1%.

Embodiment 3

[0038] 1) Take 26L of the ionizing solution after the chromatography in Example 1, open the simulated moving bed sample injection treatment, adjust the water inlet rate to be 57.40ml / min, the sample injection rate to be 17.22ml / min, and the waste water outflow rate to be 57.40ml / min. min, the product outflow rate was 17.22ml / min, and the backfill rate was 45.92ml / min; the obtained 34.9L L-ribose-rich solution was light yellow, the L-ribose content was 8.14%, the dry matter was 9.01%, and the purity was 90.3% , the recovery rate of L-ribose was 92%;

[0039] 2) Concentrating and crystallizing the solution rich in L-ribose to obtain L-ribose with a purity of 95.4%.

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Abstract

The invention relates to a method for separating and extracting L-ribose from a reaction liquid in which L-Arabitol is used for synthesizing the L-ribose, comprising the following steps of centrifuging the reaction liquid, adopting ion-exchange chromatography and moving bed (SMB) chromatography, concentrating and crystallizing to obtain the L-ribose. The purity of the L-ribose is more than 95% after crystallization. The invention has the characteristics of simple operation, high product purity, light color, high yield and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical product separation, and in particular relates to a method for separating and extracting L-ribose, in particular to a reaction for separating and extracting L-arabinose to synthesize L-ribose by using ion exchange chromatography and simulated moving bed chromatography. A method for L-ribose in liquid. Background technique [0002] L-ribose is an important saccharide related to biological heredity. It plays a very important role in physiology. It is the constituent sugar of various ribonucleic acid (RNA) and various nucleotide coenzymes. It is widely used in food and cosmetics. The industry has high application value. In recent years, with the gradual deepening of genetic engineering research, the reports on the application of L-ribose and its derivatives in medicine have been increasing. Great superiority has been shown in the study, and it is less toxic than D-ribonucleosides. Drugs with L-...

Claims

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Application Information

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IPC IPC(8): C07H3/02C07H1/06
Inventor 李荣杰薛培俭尚海涛吴鹏举何玉春马丽
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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