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Bacterial strain for producing D-arabitol and application thereof

A technology of arabitol and seeds, applied in fungi, methods based on microorganisms, microorganisms, etc., can solve problems such as pollution, increased extraction cost, and increased extraction difficulty, and achieve the effect of wide application prospects

Active Publication Date: 2020-03-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of D-arabinitol in the natural state, the extraction difficulty increases, the extraction efficiency is low, and the substrate is expensive, resulting in an increase in extraction costs
At present, the industrial production of D-arabitol at home and abroad mainly adopts the chemical synthesis method. This method is direct, has few by-products, and is easy to extract. However, the production process is complicated, energy consumption is large, the substrate is expensive, and the pollution is serious.

Method used

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  • Bacterial strain for producing D-arabitol and application thereof
  • Bacterial strain for producing D-arabitol and application thereof
  • Bacterial strain for producing D-arabitol and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: ARTP mutagenesis treatment of starting strains and screening of high-yield D-arabitol strains

[0031] The starting strain Candida parapsilosis (Candida parapsilosis) SK26.002 was inoculated into the seed medium, and cultured at 200 rpm at 30° C. for 21 h. Take 1 mL of the bacterial solution in a 1.5 mL centrifuge tube, centrifuge at 12,000 rpm for 5 min, discard the supernatant, and resuspend the bacterial cells with 1 mL of 0.9% sterile saline, centrifuge and wash the bacterial cells again to make a bacterial suspension. Then take 10 μL of the bacterial suspension and spread it evenly on the surface of the metal slide, place the slide in the ARTP mutagenesis system, place the slide in the corresponding grooves with sterile tweezers, and set the plasma mutagenesis time as 140s, after the sample processing is completed, use sterile tweezers to move the slide to a 1.5mL centrifuge tube filled with 1mL sterile saline, and shake evenly on the shaker. The new ba...

Embodiment 2

[0033] Example 2: Comparison of strain D-arabitol production before and after mutagenesis

[0034]Use an inoculation loop to dip a ring of bacteria solution of the starting strain SK26.002 and the mutant strain SK26.002A6 from the preserved bacteria solution in the glycerol tube, respectively, streak on the plate medium, and incubate them upside down in a 30°C incubator for 24 hours. Pick a single colony of the starting strain SK26.002 and a single colony of the mutant strain SK26.002 A6 that have grown well, and inoculate them in the seed medium, cultivate them at 30°C and 200rpm for 21 hours, and then inoculate the seed solution with 4% inoculum in the In a 250mL Erlenmeyer flask containing 50mL of fermentation medium, culture at 30°C and 200rpm for 72h. After the fermentation, the bacterial liquid was collected and centrifuged at 8000 rpm for 10 min to obtain a supernatant liquid containing D-arabitol. The supernatant was filtered through a 0.22 μm microporous membrane, an...

Embodiment 3

[0036] Example 3: Comparison of carbon source utilization patterns of strains before and after mutagenesis

[0037] Using glucose, fructose, sucrose, galactose, maltose, starch, lactose, glycerol and xylose as carbon sources, the carbon source utilization patterns of the starting strain and the Candida parapsilosis mutant strain SK26.002 A6 were compared.

[0038] Prepare medium containing 100 g / L of the above-mentioned carbon source and 20 g / L of yeast extract respectively, and distribute them in 20×200 mm test tubes with 10 mL aliquots, inoculate the respective seed cultures, and incubate at 30 Cultivate for 48 hours at 200 rpm. The carbon source utilization pattern of the strains before and after mutagenesis was analyzed by measuring cell growth and product formation.

[0039] The results showed that both the mutant strain and the starting strain had the ability to utilize glucose, fructose, sucrose, galactose, maltose, starch, lactose or glycerol for growth, and neither h...

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Abstract

The invention discloses a strain for producing D-arabitol and application of the strain, belonging to the technical field of biology. The invention provides Candida parapsilosis SK26.002A6 obtained bymutation breeding. The Candida parapsilosis SK26.002A6 is preserved in the China Center for Type Culture Collection (CCTCC) at present, with an accession number of CCTCC NO: M2019518. The strain canproduce D-arabitol at high yield; a production method has the advantages of mild production conditions, environmental friendliness, low cost and the like, and a new method is provided for industrial preparation of D-arabitol; and the D-arabitol produced by the method is safe and reliable, is a functional product with great market potential, and has good application prospects in the fields of food,medicines, chemical engineering and the like.

Description

technical field [0001] The invention relates to a strain producing D-arabitol and its application, especially the application in producing D-arabitol, which belongs to the field of biotechnology. Background technique [0002] D-arabitol is an important pentitol, which mainly exists in lichens and mushrooms in nature, has a sweet taste similar to sucrose, has a lower calorie content, and can inhibit the growth of cariogenic bacteria and Acid production. D-arabinitol can produce a significant cooling sensation in the oral cavity due to its endothermic dissolution, and can be a potential substitute for xylitol and other anti-caries polyols such as sorbitol and erythritol. At the same time, D-arabitol can be used as a transport medium through the blood-brain barrier and used as a drug intermediate for some drug synthesis. Therefore, D-arabinitol has a great application prospect in diabetic supplies, oral health care and pharmaceutical industry. D-arabitol has been listed as o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/18C12R1/72
CPCC12N1/16C12P7/18C12N1/165C12R2001/72
Inventor 江波张涛郑思梦
Owner JIANGNAN UNIV
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