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Strain HD924 for producing dihydroxyacetone by microbial fermentation and method

A kind of technology of dihydroxyacetone and HD924, applied in the field of fermenting glycerol to produce dihydroxyacetone

Active Publication Date: 2012-03-28
CHANGXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on microbial resources with high yield of dihydroxyacetone at home and abroad

Method used

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  • Strain HD924 for producing dihydroxyacetone by microbial fermentation and method
  • Strain HD924 for producing dihydroxyacetone by microbial fermentation and method
  • Strain HD924 for producing dihydroxyacetone by microbial fermentation and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for producing dihydroxyacetone by fermenting glycerol with the bacterial strain HD924 is specifically as follows: the bacterial strain HD924 is first inoculated into a primary seed medium with a 10% inoculum size (the composition of the primary seed medium is: glycerol 30g / L, yeast powder 15g / L, KH 2 PO 4 0.3g / L, pH natural), cultured aerobically at 28°C for 24h; then the cultured primary seeds were inserted into the secondary seed medium at an inoculum size of 5% (the composition of the secondary seed medium was : Glycerin 30g / L, yeast powder 15g / L, CaCO 3 0.3g / L, pH 6.0), after 8 hours, put the cultured secondary seeds into the fermentation medium containing different glycerin concentrations (250mL triangular flask, liquid volume 50mL) at 200 rpm / min, fermented at 28°C for 48 h, and measured the content of dihydroxyacetone. The experimental results are shown in Table 4.

[0040] Wherein, the composition of the fermentation medium is: glycerol 50~350g...

Embodiment 2

[0043] Put the cultured secondary seeds into 5% inoculum of glycerol (glycerin comes from food grade glycerol, industrial grade glycerol and saponified crude glycerol respectively) into the fermentation medium with a concentration of 100g / L (250mL Erlenmeyer flask, filled with liquid volume 50mL) at 200 rpm, fermented at 28°C for 72 hours, and measured the content of dihydroxyacetone. The experimental results are shown in Table 5. Wherein, the fermentation medium consists of: glycerol 100g / L, yeast powder 10g / L, CaCO 3 3g / L, pH 6.0; the purity of food grade glycerin is 99.5%, the purity of industrial grade glycerin is 95%, and the saponified crude glycerin is waste glycerin from soap making industry (purity 60%). One, the cultivation of secondary seed is identical with embodiment 1.

[0044]

Embodiment 3

[0046] Utilize the method for producing dihydroxyacetone by fermenting glycerol with the bacterial strain HD924, specifically: the bacterial strain HD924 is directly inserted into the fermentation medium (250mL triangular flask, liquid capacity 50mL; the fermentation medium consists of: Food grade glycerol 100g / L, peptone 8g / L, calcium carbonate 5g / L, culture medium pH 7), rotating speed 200 rpm, fermented at 30°C for 72 h under aerobic conditions; The content of hydroxyacetone was 67.4 g / L.

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PUM

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Abstract

The invention relates to a strain HD924 for producing dihydroxyacetone by microbial fermentation. The strain HD924 is classified into Acetobacteraceae Gluconobacter Gluconobacterfrateurii, and its collection number in the common micro-organism center of the CCCCM is CGMCC No.5397. The invention also provides a method for producing dihydroxyacetone by using the strain to ferment glycerin. dihydroxyacetone can be remarkably accumulated under aerobic conditions with the maximum of 170g / L. By the adoption of the method provided by the invention, industrial production can be realized.

Description

technical field [0001] The invention belongs to the field of biomanufacturing, and in particular relates to a bacterial strain HD924 for producing dihydroxyacetone by fermenting glycerin with an aerobic microorganism and a method for producing dihydroxyacetone by fermenting glycerin with the bacterial strain. Background technique [0002] Dihydroxyacetone, also known as 1,3-dihydroxyacetone, English name is 1,3-dihydroxyacetone or dihydroxyacetone, abbreviated as DHA, molecular formula is C 3 h 6 o 3 , with a molecular weight of 90.08, is the simplest three-carbon ketose. Its appearance is white or off-white powder crystals, with a sweet and cool taste, and it is easy to absorb moisture and decompose. In general, it is a dimer (1,4-Dioxane) crystal, which becomes a monomer after being dissolved or heated. [0003] Dihydroxyacetone is a naturally occurring, water-soluble and simplest ketose sugar. It is biodegradable, edible and non-toxic to humans and the environment. It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/26C12R1/01
Inventor 刘宇鹏陈万河王磊李华孙杨
Owner CHANGXING PHARMA
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