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Method for preparing xylitol and L-arabinose mixed crystal from xylose mother liquid

A technology of arabinose and xylose mother liquor, applied in the biological field, can solve the problems of complex production operations, large equipment investment, and reduced corporate profits, and achieve the effects of solving xylose mother liquor treatment, reducing production costs, and reducing energy consumption

Inactive Publication Date: 2010-12-22
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to its high sugar concentration, its viscosity is very high, and its composition is relatively complex. In addition to the above-mentioned monosaccharides, it also contains a large amount of pigments and colloids (Applied Chemical Industry, Vol. 38, No. 1, 2009, pages 8-10) , the xylose in it can no longer be separated by current crystallization techniques
my country produced about 40,000 tons of xylose mother liquor in 2009, mostly as low-priced raw materials, such as raw materials for the preparation of caramel pigments (China Food Additives, No. 5, 2002, pages 54-56; Chinese Condiments, 2007 2, pp. 54-59), greatly reducing corporate profits
The method that this invention needs to adopt is relatively complicated, and needs to go through more than 2 steps such as concentration and crystallization, and also needs to use simulated mobile separation, which is relatively complicated in actual production operation and requires a lot of equipment investment

Method used

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  • Method for preparing xylitol and L-arabinose mixed crystal from xylose mother liquid
  • Method for preparing xylitol and L-arabinose mixed crystal from xylose mother liquid
  • Method for preparing xylitol and L-arabinose mixed crystal from xylose mother liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Measure 1000 milliliters of xylose mother liquor, and measure the refractive index to be 67.4. This mother liquor is the residual dark brown viscous liquid that cannot recrystallize after preparing xylose with corncob hydrolyzate, the content of xylose is 45% (mass volume percentage), the content of L-arabinose is 15% (mass volume percentage ), the content of glucose is 12% (mass volume percentage), and the rest are other unknown miscellaneous sugars. Add 20 grams of powdered activated carbon (Chinese medicine), decolorize at 80° C. for 90 minutes, rotate and stir at 90 rpm. Then use filter cloth to filter to obtain a light brown viscous liquid, the measured refractive index is 62.2, and the reduced refractive index of 5.2 is the loss of activated carbon adsorption, and the yield is 92.2%. The decolorized mother liquor was diluted 4 times with pure water, the measured refractive index was 15.1, and the electrical conductivity was 82.4 μS / cm. Add 1.5% yeast powder by m...

Embodiment 2

[0044] Measure 500 milliliters of xylose mother liquor, this xylose mother liquor is with embodiment 1. Diluted 8 times with pure water, the measured refractive index is 8.5, and the conductivity is 76.5. Yeast powder with a mass percentage of 1.5% was added, fully dissolved, and the measured conductivity was 1.6mS / cm. The large increase in conductivity was caused by the added yeast powder. Put the prepared fermented liquid into a 5-liter fermenter, and filter to sterilize. The cultivation of Candida ssp. X828 seeds is the same as in Example 1. Then the seed solution was inoculated into a fermenter according to 20% (V / V) for fermentation, and fermented at 32° C. and 300 rpm for 48 hours. Yeast thalline was separated by centrifugation to obtain a tea-brown fermented liquid. Concentrate by rotation to a volume of 2 liters, add 20 grams of powdered activated carbon (Sinopharm), decolorize at 80° C. for 90 minutes, and rotate and stir at 90 rpm. Then use a filter cloth to filt...

Embodiment 3

[0046]The yeast cell part that embodiment 1 obtains is suspended in the xylose mother liquor diluted 5 times with pure water, is packed in the shaking flask of 2 liters, and charging 400 milliliters, fully mixes, and xylose mother liquor is the same as embodiment 1. After mixing, measure the optical density of the cells at OD600, and the measured value is 108. The cells were converted to xylose mother liquor at 32° C. and 220 rpm. During the conversion, samples were taken every 6 hours for HPLC analysis of sugar alcohol content therein. At 48 hours, xylose was basically converted to xylitol, and about 15% of L-arabinose was converted to L-arabitol. The contents of xylitol, L-arabinose and L-arabitol in the conversion solution were 58.6%, 21.2% and 9.5%, respectively. The HPLC chromatogram when transforming 48 hours is as Figure 4 shown. The decolorization, desalination and crystallization steps of the conversion solution are the same as in Example 1. 14.2 grams of mixed ...

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Abstract

The invention relates to a method for preparing rare sugar alcohol, which belongs to the field of biotechnology. The invention describes a method for preparing a xylitol and L-arabinose mixed crystal from xylose mother liquid by a biological method. The method comprises the following steps of: inoculating yeast (Candida sp)X828 CGMCC No.3691 which cannot use L-arabinose as a unique carbon source to a xylose mother liquid culture medium to ferment; consuming glucose and galactose in the xylose mother liquid; reducing the xylose to obtain xylitol so as to obtain xylitol and L-arabinose-containing fermentation liquid; and concentrating, desalting, reconcentrating and crystallizing the fermentation liquid to obtain the xylitol and L-arabinose mixed crystal. In the method, an additional value of the xylose mother liquid can be increased; and the xylitol and L-arabinose mixed crystal can be directly used for preparing a product which comprises the L-arabinose and the xylitol at the same time.

Description

technical field [0001] The invention relates to a method for preparing rare sugar alcohols in the field of biotechnology. A fermentation method using yeast (Candidasp) X828 CGMCC No.3691 to purify xylose mother liquor, consume sugars other than L-arabinose and xylose in xylose mother liquor, and convert xylose into xylitol at the same time Method for enriching L-arabinose. Background technique [0002] The acid hydrolyzate of bagasse and corncob undergoes purification processes such as neutralization, decolorization, ion exchange, and concentration to obtain a xylose-rich massecuite, and the tea-brown viscous liquid left after the xylose is separated by gradient cooling crystallization is the xylose mother liquor. On average, one ton of xylose is obtained for every ton of xylose separated by crystallization. Xylose mother liquor is rich in xylose, L-arabinose, glucose and D-galactose, and its content varies depending on the source of xylose mother liquor and different batc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C07H3/02C07H1/08A23L1/30A61K31/7004A61P3/04A61P3/10C12R1/72A23L33/125
Inventor 姜明国杨立芳王犇
Owner GUANGXI UNIV FOR NATITIES
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