D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling

A production method, continuous chromatography technology, applied in the field of D-tagatose production, can solve the problems that cannot be used to improve the conversion rate of D-galactose to D-tagatose, cannot change the chemical balance, etc., to overcome feedback Inhibition, equipment cost reduction, and process simplification effects

Active Publication Date: 2014-07-16
WENZHOU UNIVERSITY
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the reaction is still carried out in a batch tank reactor, this separation cannot change the chemical equilibrium, nor can it be used to improve the conversion rate of D-galactose to D-tagatose, and the yield of D-tagatose is only 40%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling
  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling
  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.1 U / ml, and the pH value is 6; preheating temperature, 60°C; column temperature, 60°C; column number and distribution, 8, 2 / 2 / 2 / 2 distribution; switching time, 60 min; flow rate in zone I is 1.4, flow rate in zone II is 0.8, flow rate in zone III is 0.807, and flow rate in zone IV is 0.4.

[0067] Results: The feed liquid treatment rate was 0.59 ml / min, the conversion rate of D-galactose was 82.9%, the yield of D-tagatose was 82.0%, and the purity of D-tagatose in the product solution was 99.5%.

Embodiment 2

[0069] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.18 U / ml, and the pH value is 7.4; preheating temperature, 70°C; column temperature, 70°C; column number and distribution, 8, 1 / 3 / 3 / 1 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.4, flow rate in zone III is 0.6, and flow rate in zone IV is 0.4.

[0070] Results: The feed liquid treatment rate was 12.7 ml / min, the conversion rate of D-galactose was 94.8%, the yield of D-tagatose was 94.8%, and the purity of D-tagatose in the product solution was 95.0%.

Embodiment 3

[0072]Operating conditions: the concentration of D-galactose in the feed solution is 100 g / l, the enzyme activity unit is 0.28 U / ml, and the pH value is 7; preheating temperature, 80°C; column temperature, 80°C; column number and distribution, 16, 2 / 6 / 6 / 2 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.5, flow rate in zone III is 0.93, and flow rate in zone IV is 0.35.

[0073] Results: The feed liquid treatment rate was 27.3 ml / min, the conversion rate of D-galactose was 81.6%, the yield of D-tagatose was 80.9%, and the purity of D-tagatose in the product solution was 95.0%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
glass transition temperatureaaaaaaaaaa
particle diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of biology and chemical industry, and particularly discloses a method for continuously producing D-tagatose by adopting D-galactose as a raw material on the basis of enzymatic isomerization reaction and a continuous chromatographic separation in-situ coupling technology. The method comprises the following steps of preparing a buffering solution; adequately dissolving the thermophilic L-arabinose isomerase in the water to obtain an enzyme solution; adequately dissolving D-galactose in the water to obtain a raw material solution; mixing and preheating the raw material solution, the buffering solution and the enzyme solution to obtain a feeding solution of a simulated moving bed reactor (SMBR); and placing the feeding solution into the SMBR to obtain a D-tagatose product solution through the in-situ reaction and separation. By determining the appropriate operating condition of the SMBR, the conversion rate of the D-galactose reaches more than 80 percent, the purity of the D-tagatose reaches more than 95 percent, and the yield is more than 80 percent. The method is low in cost, less in pollution and high in conversion rate; moreover, continuity in production can be realized, the consistency of the product quality can be guaranteed, and the commercialized application prospect is good.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a D-tagatose production method based on in-situ coupling of enzymatic isomerization reaction and continuous chromatographic separation. Background technique [0002] Tagatose is a six-carbon ketose sugar with the molecular formula C 6 h 12 o 6 , is the epimer of fructose and the aldehyde and ketone isomer of galactose. The molecular weight of tagatose is 180g / mol. It is a white crystal under normal conditions. The melting point and glass transition temperature are 134 ℃ and 15 ℃ respectively. It is soluble in water and slightly soluble in ethanol, and has good acid stability. Compared with sweeteners such as xylitol, D-tagatose has the characteristics of better taste, stronger processability, more health effects and higher safety. It is an excellent food sweetener. D-tagatose was officially approved by the US Food and Drug Administration (US FDA) as a generally recognized ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/02C07H3/02C07H1/06
Inventor 徐进余卫芳周慧君
Owner WENZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap