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D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling

A production method, continuous chromatography technology, applied in the field of D-tagatose production, can solve the problems that cannot be used to improve the conversion rate of D-galactose to D-tagatose, cannot change the chemical balance, etc., to overcome feedback Inhibition, equipment cost reduction, and process simplification effects

Active Publication Date: 2014-07-16
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the reaction is still carried out in a batch tank reactor, this separation cannot change the chemical equilibrium, nor can it be used to improve the conversion rate of D-galactose to D-tagatose, and the yield of D-tagatose is only 40%

Method used

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  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling
  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling
  • D-tagatose production method based on enzymatic isomerization reaction and continuous chromatographic separation in-situ coupling

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Experimental program
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Effect test

Embodiment 1

[0066] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.1 U / ml, and the pH value is 6; preheating temperature, 60°C; column temperature, 60°C; column number and distribution, 8, 2 / 2 / 2 / 2 distribution; switching time, 60 min; flow rate in zone I is 1.4, flow rate in zone II is 0.8, flow rate in zone III is 0.807, and flow rate in zone IV is 0.4.

[0067] Results: The feed liquid treatment rate was 0.59 ml / min, the conversion rate of D-galactose was 82.9%, the yield of D-tagatose was 82.0%, and the purity of D-tagatose in the product solution was 99.5%.

Embodiment 2

[0069] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.18 U / ml, and the pH value is 7.4; preheating temperature, 70°C; column temperature, 70°C; column number and distribution, 8, 1 / 3 / 3 / 1 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.4, flow rate in zone III is 0.6, and flow rate in zone IV is 0.4.

[0070] Results: The feed liquid treatment rate was 12.7 ml / min, the conversion rate of D-galactose was 94.8%, the yield of D-tagatose was 94.8%, and the purity of D-tagatose in the product solution was 95.0%.

Embodiment 3

[0072]Operating conditions: the concentration of D-galactose in the feed solution is 100 g / l, the enzyme activity unit is 0.28 U / ml, and the pH value is 7; preheating temperature, 80°C; column temperature, 80°C; column number and distribution, 16, 2 / 6 / 6 / 2 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.5, flow rate in zone III is 0.93, and flow rate in zone IV is 0.35.

[0073] Results: The feed liquid treatment rate was 27.3 ml / min, the conversion rate of D-galactose was 81.6%, the yield of D-tagatose was 80.9%, and the purity of D-tagatose in the product solution was 95.0%.

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Abstract

The invention belongs to the field of biology and chemical industry, and particularly discloses a method for continuously producing D-tagatose by adopting D-galactose as a raw material on the basis of enzymatic isomerization reaction and a continuous chromatographic separation in-situ coupling technology. The method comprises the following steps of preparing a buffering solution; adequately dissolving the thermophilic L-arabinose isomerase in the water to obtain an enzyme solution; adequately dissolving D-galactose in the water to obtain a raw material solution; mixing and preheating the raw material solution, the buffering solution and the enzyme solution to obtain a feeding solution of a simulated moving bed reactor (SMBR); and placing the feeding solution into the SMBR to obtain a D-tagatose product solution through the in-situ reaction and separation. By determining the appropriate operating condition of the SMBR, the conversion rate of the D-galactose reaches more than 80 percent, the purity of the D-tagatose reaches more than 95 percent, and the yield is more than 80 percent. The method is low in cost, less in pollution and high in conversion rate; moreover, continuity in production can be realized, the consistency of the product quality can be guaranteed, and the commercialized application prospect is good.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a D-tagatose production method based on in-situ coupling of enzymatic isomerization reaction and continuous chromatographic separation. Background technique [0002] Tagatose is a six-carbon ketose sugar with the molecular formula C 6 h 12 o 6 , is the epimer of fructose and the aldehyde and ketone isomer of galactose. The molecular weight of tagatose is 180g / mol. It is a white crystal under normal conditions. The melting point and glass transition temperature are 134 ℃ and 15 ℃ respectively. It is soluble in water and slightly soluble in ethanol, and has good acid stability. Compared with sweeteners such as xylitol, D-tagatose has the characteristics of better taste, stronger processability, more health effects and higher safety. It is an excellent food sweetener. D-tagatose was officially approved by the US Food and Drug Administration (US FDA) as a generally recognized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/02C07H3/02C07H1/06
Inventor 徐进余卫芳周慧君
Owner WENZHOU UNIVERSITY
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