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A method for the production of d-tagatose based on the in situ coupling of enzymatic isomerization and continuous chromatographic separation

A technology of continuous chromatography and production method, applied in the field of D-tagatose production, can solve the problems that cannot be used to improve the conversion rate of D-galactose to D-tagatose, and cannot change the chemical balance, so as to overcome the feedback Inhibiting effect, reducing equipment costs, reducing the effect of operating units

Active Publication Date: 2018-03-20
塔格糖生物科技产业发展湖北有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the reaction is still carried out in a batch tank reactor, this separation cannot change the chemical equilibrium, nor can it be used to improve the conversion rate of D-galactose to D-tagatose, and the yield of D-tagatose is only 40%

Method used

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  • A method for the production of d-tagatose based on the in situ coupling of enzymatic isomerization and continuous chromatographic separation
  • A method for the production of d-tagatose based on the in situ coupling of enzymatic isomerization and continuous chromatographic separation
  • A method for the production of d-tagatose based on the in situ coupling of enzymatic isomerization and continuous chromatographic separation

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Effect test

Embodiment 1

[0066] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.1 U / ml, and the pH value is 6; preheating temperature, 60°C; column temperature, 60°C; column number and distribution, 8, 2 / 2 / 2 / 2 distribution; switching time, 60 min; flow rate in zone I is 1.4, flow rate in zone II is 0.8, flow rate in zone III is 0.807, and flow rate in zone IV is 0.4.

[0067] Results: The feed liquid treatment rate was 0.59 ml / min, the conversion rate of D-galactose was 82.9%, the yield of D-tagatose was 82.0%, and the purity of D-tagatose in the product solution was 99.5%.

Embodiment 2

[0069] Operating conditions: the concentration of D-galactose in the feed liquid is 10 g / l, the enzyme activity unit is 0.18 U / ml, and the pH value is 7.4; preheating temperature, 70°C; column temperature, 70°C; column number and distribution, 8, 1 / 3 / 3 / 1 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.4, flow rate in zone III is 0.6, and flow rate in zone IV is 0.4.

[0070] Results: The feed liquid treatment rate was 12.7 ml / min, the conversion rate of D-galactose was 94.8%, the yield of D-tagatose was 94.8%, and the purity of D-tagatose in the product solution was 95.0%.

Embodiment 3

[0072]Operating conditions: the concentration of D-galactose in the feed solution is 100 g / l, the enzyme activity unit is 0.28 U / ml, and the pH value is 7; preheating temperature, 80°C; column temperature, 80°C; column number and distribution, 16, 2 / 6 / 6 / 2 distribution; switching time, 80 min; flow rate in zone I is 1.35, flow rate in zone II is 0.5, flow rate in zone III is 0.93, and flow rate in zone IV is 0.35.

[0073] Results: The feed liquid treatment rate was 27.3 ml / min, the conversion rate of D-galactose was 81.6%, the yield of D-tagatose was 80.9%, and the purity of D-tagatose in the product solution was 95.0%.

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Abstract

The invention belongs to the field of biology and chemical industry, and particularly discloses a method for continuously producing D-tagatose based on enzymatic isomerization reaction and chromatographic separation in-situ coupling technology using D-galactose as raw material, comprising the following steps: configuring Buffer solution; fully dissolve thermophilic L-arabinose isomerase in water as an enzyme solution; fully dissolve D-galactose in water as a raw material solution; mix and preheat the raw material solution, buffer solution, and enzyme solution Finally, it is used as the feed liquid of the simulated moving bed reactor (SMBR); the feed liquid enters the simulated moving bed reactor, and after in-situ reaction and separation, the D-tagatose product solution is obtained. The invention establishes suitable operating conditions for SMBR, the conversion rate of D-galactose reaches more than 80%, the purity of D-tagatose reaches more than 95%, and the yield exceeds 80%. The method disclosed by the invention has low cost, less pollution and high conversion rate, can realize continuous production, is beneficial to ensure the uniformity of product quality, and has good commercial application prospects.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a D-tagatose production method based on in-situ coupling of enzymatic isomerization reaction and continuous chromatographic separation. Background technique [0002] Tagatose is a six-carbon ketose sugar with the molecular formula C 6 h 12 o 6 , is the epimer of fructose and the aldehyde and ketone isomer of galactose. The molecular weight of tagatose is 180g / mol. It is a white crystal under normal conditions. Its melting point and glass transition temperature are 134°C and 15°C respectively. It is soluble in water, slightly soluble in ethanol, and has good acid stability. Compared with sweeteners such as xylitol, D-tagatose has the characteristics of better taste, stronger processability, more health effects and higher safety. It is an excellent food sweetener. D-tagatose was officially approved by the US Food and Drug Administration (US FDA) as a generally recognized as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/24C12P19/02C07H3/02C07H1/06
Inventor 徐进余卫芳周慧君
Owner 塔格糖生物科技产业发展湖北有限公司
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