Method for purifying tagatose by using sequential simulated moving bed chromatography (SSMB)

A technology for simulating mobile chromatography and tagatose, which is applied in the field of sequential simulated mobile chromatography to purify tagatose, can solve the problem that tagatose cannot be efficiently purified, and achieves the advantages of increasing resin utilization rate, reducing concentration cost and reducing equipment investment. Effect

Active Publication Date: 2014-08-20
HEILONGJIANG HAOTIAN CORN DEV
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the difficult problem that tagatose cannot be efficiently purified, and a method for purifying tagatose by sequential simulated mobile chromatography has been studied, comprising the following steps:

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for purifying tagatose by using sequential simulated moving bed chromatography (SSMB)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Such as figure 1 As shown, the sequential analog mobile chromatographic equipment used in the present invention consists of 6 chromatographic columns (inner diameter 35mm, length 1000mm), each chromatographic column is 1L, a total of 6L, and the inlet and outlet are controlled by solenoid valves. Achieved through the following steps:

[0021] A) Blending of feed syrup: blend the desalted and decolorized tagatose syrup to a concentration of 60%;

[0022] B) Filtration: filter the syrup solution obtained in step A) with a 50 micron filter to obtain a clear liquid without visible impurities;

[0023] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment, and the purification method is as follows:

[0024] a) Simultaneously feed materials, feed eluent, and discharge materials. Column 1 enters eluent D (deionized water), and component A (tagatose component) exits from the...

Embodiment 2

[0031] In the method for purifying tagatose by sequential simulated mobile chromatography of the present invention, the test adopts sequential simulated mobile chromatography equipment for purification. The sequential simulated mobile chromatography equipment used in the present invention is composed of 6 chromatographic columns (35 mm in inner diameter and 1000 mm in length). Each chromatographic column is 1L, a total of 6L, and the material in and out is controlled by a solenoid valve. It is achieved through the following steps:

[0032] A) operate according to step A) in embodiment 1;

[0033] B) operate according to step B) in embodiment 1;

[0034] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment, and the purification method is as follows:

[0035] a) Simultaneously feed materials, feed eluent, and discharge materials. Column 1 enters eluent D (deionized water), a...

Embodiment 3

[0042] In the method for purifying tagatose by sequential simulated mobile chromatography of the present invention, the test adopts sequential simulated mobile chromatography equipment for purification. The sequential simulated mobile chromatography equipment used in the present invention is composed of 6 chromatographic columns (35 mm in inner diameter and 1000 mm in length). Each chromatographic column is 1L, a total of 6L, and the material in and out is controlled by a solenoid valve. It is achieved through the following steps:

[0043] A) operate according to step A) in embodiment 1;

[0044] B) operate according to step B) in embodiment 1;

[0045] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment, and the purification steps are as follows:

[0046] a) Simultaneously feed materials, feed eluent, and discharge materials. Column 1 enters eluent D (deionized water), a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for purifying tagatose by using sequential simulated moving bed chromatography (SSMB). Pretreated tagatose crude syrup is used as a raw material, an SSMB is utilized to purify the tagatose, deionized water is used as eluting agent, the working temperature of the separating column is 50-70 DEG C, and a calcium-type strongly-acidic cation gel resin is used as a chromatographic stationary phase, thereby obtaining the high-purity tagatose and implementing higher yield. The whole technique has the advantages of low operating cost, low production energy consumption, zero discharge and high degree of automation, and can implement continuous production and enhance production efficiency.

Description

technical field [0001] The invention belongs to the field of food industry, and specifically relates to a sequential simulated mobile chromatography separation and purification technology, in particular to a sequential simulated mobile chromatography purification technology for tagatose. Background technique [0002] Tagatose is a rare natural ketohexose, an isomer of galacturosone, and an epimer of fructose at the C-4 position. Tagatose products have been approved for use by the food hygiene departments of the United States, Australia, Japan, South Korea, and New Zealand, but have not yet been industrialized in my country. Our country has also carried out some research work on tagatose. Chinese patent 200910307138.5 has announced a kind of method for preparing L-tagatose, and what this method has adopted is the method of fermentation; Chinese patent 201080067326.6 has announced a kind of method for preparing tagatose and glucose, and its method is that Hydrolysis to galac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/02C07H1/06
Inventor 李良玉于伟贾鹏禹李朝阳井雪莲张丽萍吕获柱
Owner HEILONGJIANG HAOTIAN CORN DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap