Preparation method of polysaccharide in pine cone from Pinus koraiensis

A technology of polysaccharides and pine cones, which is applied in the field of preparation of neutral polysaccharides from Korean pine cones, can solve the problems of inability to completely remove weak and medium polar chemical components, low protein removal rate, etc., and achieve ABTS free removal base and chelate Fe2+, inhibit growth and reproduction, and improve quality

Inactive Publication Date: 2011-04-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that the weak and medium polar chemical components in the Korean pine cone cannot be completely removed in the existing preparation m

Method used

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  • Preparation method of polysaccharide in pine cone from Pinus koraiensis
  • Preparation method of polysaccharide in pine cone from Pinus koraiensis
  • Preparation method of polysaccharide in pine cone from Pinus koraiensis

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specific Embodiment approach 1

[0013] Specific embodiment one: the preparation method of a kind of Korean pine cone polysaccharide of present embodiment, carry out according to the following steps: one, degreasing: the Korean pine pine cone is pulverized, and the mass concentration that adds the Korean pine pine cone volume 5~8 times is 95 % ethanol solution, processed in slightly boiling state for 2 to 4 hours, filtered, and repeated the above-mentioned treatment and filtration steps for 2 to 3 times, dried to obtain degreased Korean pine tower; 2. Extraction: to degreased Korean pine Add distilled water in the tower, the volume ratio of the Korean pine tower and the distilled water is 1: 10~12, extract 2~3 times under the condition of 100 ℃, extract 2~4h each time, filter to obtain the Korean pine tower water extract; , preparation of crude polysaccharide: concentrating the Korean pine tower water extract to 10% to 13% of the original volume to obtain a concentrated solution, then adding a mass concentrati...

specific Embodiment approach 2

[0016] Specific embodiment two: the difference between this embodiment and specific embodiment one is: add the mass concentration of 6~7 times of the volume of the Korean pine pine cone in the step one and be 95% ethanol solution. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one is: add the mass concentration of 5 times of the volume of the Korean pine pine tower in the step one and be the ethanol solution of 95%. Others are the same as in the first embodiment.

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Abstract

The invention discloses a preparation method of polysaccharide in pine cone from Pinus koraiensis, relating to a preparation method of polysaccharide in pine cone from Pinus koraiensis. The invention solves the problems that the traditional preparation method of the polysaccharide in pine cone from Pinus koraiensis cannot completely remove chemical components with weak polarity and medium polarity in the pine cone from Pinus koraiensis and the protein removal rate is relatively lower. The preparation method comprises the following steps: 1. degreasing; 2. extracting; 3. preparing coarse polysaccharide; 4. deproteinizing; and 5. decoloring, collecting precipitates, freezing and drying to obtain the refined polysaccharide in pine cone from Pinus koraiensis. The extraction rate of the neutral polysaccharide in pine cone from Pinus koraiensis prepared by the invention can reach 1.15-1.52 percent, the protein removal rate is up to over 70 percent and the pigment removal rate is up to over 95 percent. The chromatography test shows that the neutral polysaccharide mainly comprises seven monosaccharides, namely D-ribose, L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose and D-galactose, and the preparation method of polysaccharide in pine cone from Pinus koraiensis is applied to the field of preparation of neutral polysaccharide pine cone from Pinus koraiensis.

Description

technical field [0001] The invention relates to a preparation method of the neutral polysaccharide of Korean pine cones. Background technique [0002] Pine cones are the cones of Pinaceae (Pinaceae) plants of the genus Pinus. Pinaceae plants are widely distributed in my country, rich in resources, and have considerable development potential. Especially in the Greater and Lesser Khingan Mountains forest area in my country, there are rich forest resources. Among them, the Lesser Khingan Mountains forest area in the east of Heilongjiang Province is even more famous as the "hometown of Korean pine". "Compendium of Materia Medica" records the origin, shape, efficacy and indications of pine leaves and pine cones. Among them, pine cones have the functions of eliminating phlegm, relieving cough and asthma, dispelling wind, moistening intestines, antibacterial and anti-inflammatory. [0003] At present, most of the polysaccharides extracted and separated from Korean pine cones are a...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杨鑫赵海田王静蒙琦徐韧博
Owner HARBIN INST OF TECH
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