Extraction method and application of Pinus koraiensis pine needle polyphenol compound

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an extraction method and application of a pinus koraiensis pine needle polyphenol compound. The extraction method comprises the steps of 1, pretreating pinus koraiensis pine needles, performing drying in the sun, performing cleaning, performing sterilization, and then adding the sterilized pinus koraiensis pine needles into a constant-temperature drying box at the temperature of 40 DEG C till complete drying is achieved; 2, putting the treated pinus koraiensis pine needles into a high-speed crusher for crushing, putting the completely dried pinus koraiensis pine needles into the high-speed crusher for electric crushing, and then performing screening through a 60-mesh sieve; and 3, taking a proper amount of pine needle powder, performing high-shear extraction according to a certain material-liquid ratio, performing centrifugal filtration to obtain a pine needle polyphenol extracting solution, selecting the pine needle powder and an ethanol solution with the purity of 60% according to a ratio of 1: 10, adding the pine needle powder and the ethanol solution into a container, and performing extraction by a high-shear emulsification dispersion machine at 40 DEG C. The pine needle polyphenol extracting solution is reasonable in structure, high in extraction rate, short in extraction time, low in solvent consumption and low in tool wear.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for extracting polyphenolic compounds from pine needles of Korean pine and its application. Background technique [0002] Pine needles are the leaves of the gymnosperms Pinaceae Pinus, which are extremely rich in resources in my country, but the leaves of Pinus plants are rarely developed and utilized, resulting in a large waste of resources. The development of domestic pine needle tea is relatively late, and the process is simple. The dried pine needles after initial processing are mainly made into tea for drinking. Long-term drinking of pine needle tea can play a certain role in lowering blood pressure and blood sugar. Domestic scholars believe that the functional components in pine needles are phenolic acid components, mainly pine needle polyphenols. For the extraction process of pine needle polyphenols, most domestic literatures use a certain volume fraction of ...

AI Technical Summary

Problems solved by technology

However, the influence of these methods on the extraction effect of pine polyphenol by sonic wave-compound enzyme method

But these methods all have shortcoming, for example: solvent consumption is big, extraction time is longer and extraction rate is low

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