Method for identifying stigma maydis polysaccharide by utilizing thin layer chromatography
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A corn silk polysaccharide and thin-layer chromatography technology, which is applied in the field of drug identification and achieves the effects of good separation, clear spot color, and good popularity of instruments, equipment and materials.
Inactive Publication Date: 2012-07-25
JILIN INST OF CHEM TECH
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Embodiment 1
[0044] 1. Polysaccharide hydrolysis: Take 1 mg of crude polysaccharide and dissolve it in 5 ml of 2mol / L trifluoroacetic acid solution, and place it in a reaction kettle for hydrolysis at 120°C for 3 hours. After cooling, vacuum filter to obtain the filtrate, spin dry under reduced pressure, add 1.5ml of 50% ethanol to dissolve, and obtain a reddish-brown solution, which is the polysaccharide sample after hydrolysis.
[0045] 2. Polysaccharide decolorization: Take 0.5ml polysaccharide sample after hydrolysis and add 30uL H 2 o 2 , heating at 70°C for 10 minutes to decolorize. A light yellow solution is obtained, which is the polysaccharide sample after decolorization.
[0046] 3. Monosaccharide reference substance: prepare standard solutions of D-glucose, D-xylose, D-mannose, L-arabinose, α-rhamnose, and D-galactose at different concentrations. In addition, 0.3 mL of D-glucose, D-mannose, L-arabinose, α-rhamnose, D-galactose, D-galacturonic acid, and D-xylose were prepared ...
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Abstract
The invention relates to a method for identifying stigma maydis polysaccharide by utilizing thin layer chromatography and belongs to the technical field of medicine authentication. The method comprises the following steps that: a thin layer plate is prepared by silica gel G and sodium carboxymethylcellulose (CMC-Na); D-glucose, D-xylose, D-mannose, L-Arabinose, Alfa-rhamnose, D-galactose and D-galacturonic acid are used as reference substances; corn stigma polysaccharide crude product extracted by water extraction and alcohol precipitation is used as a sample, the sample is dissolved by 50% ethanol, trifluoroacetic acid is utilized to carry out hydrolysis, hydrogen peroxide is used for decoloration, normal butanol methanol, chloroform, glacial acetic acid and water are used as developing solvents, and aniline, diphenylamine, phosphoric acid and water solution are used for color development. The spot position and color of the sample and the reference substances are consistent, so that the type of saccharine contained in the corn stigma can be judged. The method has high specificity, is simple to operate and can be used for accurately identifying the type of monosaccharide in the stigma maydis polysaccharide.
Description
technical field [0001] The invention relates to a TLC identification method for corn silk polysaccharin, belonging to the field of drug identification. Background technique [0002] The "hair" of corn, that is, Stigma maydis, is the style and stigma of the grass plant Maize, and is a commonly used traditional Chinese medicine. Traditional Chinese medicine believes that corn silk has light taste, flat nature, diuretic, calming liver, and choleretic effects. Modern medical research believes that corn silk contains fatty oil, saponins, alkaloids, flavonoids, vitamin K 3 , ascorbic acid, organic acids, etc., have diuretic, hypotensive, hypoglycemic, choleretic, hemostatic, antibacterial, immune-enhancing, anti-cancer and other effects. Scholars have studied the hypoglycemic effect of its water decoction. The content of corn silk polysaccharide is 4% in corn silk, which is one of the most important water-soluble components of corn silk. A large number of experiments have prove...
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