Abelmoschus manihot polysaccharide with anti-tumor activity and preparation method thereof
A technology of anti-tumor activity and hollyhock flower, which is applied in the direction of anti-tumor drugs, organic active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of lagging research on chemical ingredients, polysaccharides, etc., and achieve no adverse reactions and good results. Antitumor activity, convenient administration effect
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Embodiment 1
[0031] 1. The preparation of hollyhock flower polysaccharide, such as figure 1 As shown in the process flow, it specifically includes the following steps:
[0032] (1) Degreasing
[0033] Weigh 400g of hollyhock flowers, soak and extract with absolute ethanol 8 times the total weight of hollyhock flowers in a water bath at 70°C for 8 hours, suction filter while hot, and dry the dregs at 60°C to obtain degreased medicinal materials ;
[0034] (2) Extraction of crude polysaccharide
[0035] Take 200 g of the degreased hollyhock medicinal material in step (1), add 10 times the amount of water, and soak and extract twice at 70 ° C for 8 hours each time. Filtrate, combine the filtrates, centrifuge at 2000rpm for 10min, and concentrate the supernatant under reduced pressure at 70°C to 1 / 10 of the original volume, then add 95% ethanol until the ethanol concentration is 70%, let it stand at 4°C for 24h, and filter out the precipitate , the precipitate was washed successively with ...
Embodiment 2
[0064] Example 2 Antitumor activity experiment of hollyhock flower polysaccharide
[0065] 1 Materials and reagents
[0066] 1.1 Main reagents DMEM medium: GIBCO, calf serum: HyClone company; trypsin: GIBCO company; MTT: American amresco company.
[0067] 1.2 Cell lines of human liver cancer SMMC-7721, HepG2, gastric cancer MGC-803, MKN-45 cells used in the experiment were donated by the Medical Oncology Laboratory of Jiangsu Provincial Hospital of Traditional Chinese Medicine.
[0068] 2 methods
[0069] 2.1 Cell culture Human liver cancer SMMC-7721, HepG2, gastric cancer MGC-803, MKN-45 cells were cultured in DMEM medium (containing penicillin 100U / ml; streptomycin 100μg / ml; 10% fetal bovine serum), placed at 37°C , cultured in a 5% CO2 constant temperature incubator, and the cells in the logarithmic growth phase were taken for experiments.
[0070] 2.2 The effect of hollyhock polysaccharides on cell proliferation Take the cells in the logarithmic growth phase as 1*10 5 ...
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