Abelmoschus manihot polysaccharide with anti-tumor activity and preparation method thereof

A technology of anti-tumor activity and hollyhock flower, which is applied in the direction of anti-tumor drugs, organic active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of lagging research on chemical ingredients, polysaccharides, etc., and achieve no adverse reactions and good results. Antitumor activity, convenient administration effect

Active Publication Date: 2013-03-13
江苏鼎泰药物研究(集团)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the relevant research on hollyhock flowers mainly focuses on the pharmacology and pharmacodynamics of flavonoids; however, the research on polysaccharides, another important chemical component in hol

Method used

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  • Abelmoschus manihot polysaccharide with anti-tumor activity and preparation method thereof
  • Abelmoschus manihot polysaccharide with anti-tumor activity and preparation method thereof
  • Abelmoschus manihot polysaccharide with anti-tumor activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. The preparation of hollyhock flower polysaccharide, such as figure 1 As shown in the process flow, it specifically includes the following steps:

[0032] (1) Degreasing

[0033] Weigh 400g of hollyhock flowers, soak and extract with absolute ethanol 8 times the total weight of hollyhock flowers in a water bath at 70°C for 8 hours, suction filter while hot, and dry the dregs at 60°C to obtain degreased medicinal materials ;

[0034] (2) Extraction of crude polysaccharide

[0035] Take 200 g of the degreased hollyhock medicinal material in step (1), add 10 times the amount of water, and soak and extract twice at 70 ° C for 8 hours each time. Filtrate, combine the filtrates, centrifuge at 2000rpm for 10min, and concentrate the supernatant under reduced pressure at 70°C to 1 / 10 of the original volume, then add 95% ethanol until the ethanol concentration is 70%, let it stand at 4°C for 24h, and filter out the precipitate , the precipitate was washed successively with ...

Embodiment 2

[0064] Example 2 Antitumor activity experiment of hollyhock flower polysaccharide

[0065] 1 Materials and reagents

[0066] 1.1 Main reagents DMEM medium: GIBCO, calf serum: HyClone company; trypsin: GIBCO company; MTT: American amresco company.

[0067] 1.2 Cell lines of human liver cancer SMMC-7721, HepG2, gastric cancer MGC-803, MKN-45 cells used in the experiment were donated by the Medical Oncology Laboratory of Jiangsu Provincial Hospital of Traditional Chinese Medicine.

[0068] 2 methods

[0069] 2.1 Cell culture Human liver cancer SMMC-7721, HepG2, gastric cancer MGC-803, MKN-45 cells were cultured in DMEM medium (containing penicillin 100U / ml; streptomycin 100μg / ml; 10% fetal bovine serum), placed at 37°C , cultured in a 5% CO2 constant temperature incubator, and the cells in the logarithmic growth phase were taken for experiments.

[0070] 2.2 The effect of hollyhock polysaccharides on cell proliferation Take the cells in the logarithmic growth phase as 1*10 5 ...

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Abstract

The invention discloses an abelmoschus manihot polysaccharide with an anti-tumor activity and a preparation method thereof. The polysaccharide is formed by beta-D-glucopyranose, alpha-D-mannopyranose, alpha-D-galactose and alpha-L-fucopyranose in a mol ratio of 1: 0.91: 2.14: 1.0, and has the molecular weight of 8867. The structural formula of a basic unit of the polysaccharide is that alpha-D-galactose (1->6) and alpha-D-mannopyranose (2->6) are taken as main chains and mannose C-3 branches are connected with the alpha-L-fucopyranose (1->3) and connected with the beta-D-glucopyranose (->1). According to the abelmoschus manihot polysaccharide disclosed by the invention, an extraction and separation process is selected through a large number of experiments; firstly, a water extraction and alcohol precipitation method is used for obtaining a rough polysaccharide and then protein is removed; and then, a DEAE-52 (Diethyl Aminoethanol-52) cellulose resin and SephadexG-100 gel resin are combined for purifying to prepare the abelmoschus manihot polysaccharide with high purity. Anti-tumor experiment results show that the abelmoschus manihot polysaccharide has very good anti-tumor activity and has no untoward effects after being used for a long period; and the abelmoschus manihot polysaccharide can be conveniently prepared into medicines with various preparations with pharmaceutically acceptable carriers, and is convenient to take clinically.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a marshmallow flower polysaccharide with antitumor activity and its preparation method and application. Background technique [0002] Hollyhock flower is the dried corolla of Malvaceae Abelmoschus manihot L.Medicus. It has the functions of clearing heat and detoxifying, removing blood stasis, reducing inflammation and analgesia. disease. At present, the relevant research on hollyhock flowers mainly focuses on the pharmacology and pharmacodynamics of flavonoids; however, the research on polysaccharides, another important chemical component in hollyhock flowers, is relatively lagging behind. So far, there have been no reports on the systematic preparation, structure identification and pharmacological activity analysis of polysaccharides from marshmallow flowers. A large amount of research data shows that natural polysaccharides have good anti-tumor biological activity, so...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 刘志辉李爽王琼祈明浩钱芳陆超严海兵
Owner 江苏鼎泰药物研究(集团)股份有限公司
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