Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product

A technology of Cordyceps sinensis polysaccharide and Cordyceps sinensis, which is applied in the field of food inspection and analysis, and achieves the effect of simple and convenient pretreatment and easy popularization

Inactive Publication Date: 2014-10-08
黄宏南
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there is no record of a rapid, accurate, and easy-to-popular detection met

Method used

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  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product
  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product
  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product

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Embodiment 1

[0026] The method provided by the invention is used to measure the polysaccharide peptide of Cordyceps sinensis in Qinghai Jiadui Cordyceps sinensis pure powder sample.

[0027] 1. The instruments and medicines used in the determination include: Waterse2695 high performance liquid chromatography system, Waterse2996 diode array detector, Empower chromatography workstation and data processing system; Cordyceps polysaccharide peptide standard (>98%, National Juncao Engineering Technology Research center);

[0028] 2. The determination steps are as follows:

[0029] (1) Sample processing

[0030] Weigh 0.25g of pure powder sample of Qinghai Jiadui Cordyceps sinensis, add 10ml of water and extract in 60°C water bath for 30min, after cooling, add water to 50ml to make it an aqueous solution of Cordyceps sinensis product with a concentration of 5.00mg / ml, filter This solution, take 2ml of filtrate, add 6ml of absolute ethanol and then centrifuge 15min by centrifuge, the rotating sp...

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Abstract

The invention relates to a method for simply, quickly, directly and accurately measuring the connect of cordyceps sinensis polysaccharide peptide in a cordyceps sinensis product. The method comprises the following steps: performing simple pretreatment on a sample, and qualitatively and quantitatively analyzing through a high performance liquid chromatography photo-diode array (HPLC-PDA) detector. By utilizing the advantages of accurate quantification of high performance liquid chromatography, accurate spectral analysis qualification of the PDA detector and the like, the method can be used for simply, quickly, accurately and directly measuring the connect of cordyceps sinensis polysaccharide peptide in the ordyceps sinensis product, and a simple, reliable and easily-popularized method is supplied to authentication analysis, quality evaluation and quality control of health efficacy factors in the cordyceps sinensis product.

Description

technical field [0001] The invention belongs to the field of food inspection and analysis, and specifically relates to a method for accurately and rapidly measuring the content of cordyceps sinensis polysaccharide peptides in cordyceps sinensis products by using the HPLC-PDA method. Background technique [0002] Cordyceps sinensis (scientific name: Ophiocordyceps sinensis), also known as Chinese caterpillar fungus, is a traditional precious Chinese medicinal material in Chinese history. It is mainly produced in the alpine regions and snow-capped grasslands of Qinghai, Tibet, Sichuan, Yunnan, Gansu and Guizhou provinces and autonomous regions in mainland China. Modern medicine has proved that Cordyceps sinensis has the effects of calming down, relieving cough and reducing phlegm, inhibiting the growth of tumor cells, increasing the body's unique immunity to fitness and disease prevention, and the polysaccharide peptide of Cordyceps sinensis is one of the main functional ingred...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 黄宏南吴志仁王娟娟
Owner 黄宏南
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