High activity golden mushroom polysaccharide-peptide-Fe chelate and its prepn

A technology of Flammulina velutipes polysaccharides and chelates, applied in chemical instruments and methods, peptides, specific peptides, etc., can solve the problems affecting the quality of iron-fortified food, food color black, poor stability, etc., to improve the immune function of the whole body, The effect of performance function synergy and high stability

Inactive Publication Date: 2007-01-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the inorganic salt of iron is used as a food fortifier, the iron has a strong fishy smell and has a fishy smell after eating, and has poor stability to heat and light. In food process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: In the process of cultivating Flammulina velutipes, ferrous sulfate is added to the fruiting medium by mixing iron and adding ferrous sulfate to the quality of the fruiting medium 9×10 -4 %. After the water in the fruiting culture medium is absorbed by the material, bag it and sterilize it. In a sterile room, the inoculum of 3.9% relative to the quality of the fruiting culture medium is connected to the original seed and cultivated in a sealed room at 25°C , Get iron-rich Flammulina velutipes fruit body;

[0033] The components and mass percentages of the fruiting culture medium are 94% cottonseed hulls, 3% wheat bran, 0.8% sucrose, and 2.2% water;

[0034]Step 2: Take the iron-rich Flammulina velutipes fruit body as raw material, homogenize it, weigh it, add water according to raw material (w): water (v) = 1:30, heat up to 87°C, keep warm and stir for 1 hour, according to raw material quality Add 1% of cellulase and papain as a composite enzyme, act for 100 min at ...

Embodiment 2

[0039] Step 1: In the process of cultivating Flammulina velutipes, ferrous gluconate is added to the fruiting medium by spraying iron, and the added amount is 11×10 of the quality of the fruiting medium. -4 %. After the water in the fruiting culture medium is absorbed by the material, bag it and sterilize it. In a sterile room, 3.6% of the inoculum relative to the quality of the fruiting culture medium is connected to the original seed and cultivated in a sealed room at 25°C , Get iron-rich Flammulina velutipes fruit body;

[0040] The components and mass percentages of the fruiting culture medium are 90% cottonseed hulls, 6% wheat bran, 2% sucrose, and 2% water;

[0041] Step 2: Take the iron-rich Flammulina velutipes fruit body as raw material, homogenize it, weigh it, add water according to raw material (w): water (v) = 1:10, heat up to 90°C, keep stirring for 2 hours, according to the quality of raw materials Add cellulase to 0.08% of cellulase at 65°C and pH=4.5 for 95min, th...

Embodiment 3

[0046] Step 1: In the process of cultivating Flammulina velutipes, ferrous lactate is added to the fruiting medium by soaking iron, and the amount added is 6×10 of the quality of the fruiting medium. -4 %. After the water in the fruiting culture medium is absorbed by the material, bag it and sterilize it. In a sterile room, the inoculum amount of 4.9% relative to the quality of the fruiting culture medium is connected to the original seed and cultivated in a sealed room at 25℃ , Get iron-rich Flammulina velutipes fruit body;

[0047] The components and mass percentages of the fruiting culture medium are 96% cottonseed hulls, 1% wheat bran, 0.5% sucrose, and 2.5% water;

[0048] Step 2: Take the iron-rich Flammulina velutipes fruit body as raw material, homogenize it, weigh it, add water according to raw material (w): water (v) = 1:40, heat up to 85°C, keep stirring for 4 hours, according to the quality of raw material Add 0.05% of the compound enzyme of cellulase, hemicellulase an...

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Abstract

The present invention is one kind of high activity golden mushroom polysaccharide-peptide- Fe2+ chelate, which has molecular weight of 20-50 KD, and contains polysaccharide in 69.9-70.2 wt%, peptide in 29.3-29.7 wt% and Fe2+ in 0.3-0.6 wt%. The golden mushroom polysaccharide-peptide- Fe2+ chelate is prepared with iron-rich sporophore as material and through the following steps: enzymolyzing with cellulose, hemicellulase or papain to break cell wall to raise the content of effective components, especially polysaccharide; the subsequent degradation of golden mushroom polysaccharide-peptide- Fe2+ chelate under the action of polysaccharidase; and final two-stage ultrafiltering membrane separation to obtain high activity golden mushroom polysaccharide-peptide- Fe2+ chelate with molecular weight of 20-50 KD. The high activity golden mushroom polysaccharide-peptide- Fe2+ chelate can improve systemic immune function, inhibit tumor and replenish iron effectively.

Description

Technical field [0001] The invention belongs to the field of functional active ingredients, and particularly relates to a highly active Flammulina velutipes polysaccharide-peptide-Fe 2+ Chelate and its preparation method. Background technique [0002] Carbohydrates are the most organic compounds in nature, as well as important biopolymer compounds and important information molecules. They participate in the regulation of various life activities of cells. Active polysaccharides refer specifically to polysaccharide compounds with a particular physiological activity. Strong immune regulation and anti-tumor activity have attracted much attention. Among them, polysaccharides have a very obvious effect on promoting and restoring the body's immune function. Fungal polysaccharide is a polysaccharide component extracted from fungi. As a broad-spectrum immunomodulator, it mainly stimulates the maturation and differentiation of various immunologically active cells in the body through the ho...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04C07K17/10
Inventor 郑建仙王超叶远寿
Owner SOUTH CHINA UNIV OF TECH
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