A method for producing high-purity intracellular glycopeptides of versicolor versicolor by using modern fermentation technology

A technology of versicolor intracellular glycopeptide and fermentation tank, which is applied in the direction of peptide preparation method, microorganism-based method, biochemical equipment and method, etc., can solve the problem of low yield of versicolor intracellular glycopeptide, and achieve reduction Effect of heating extraction time, benefiting yield, improving yield and quality

Inactive Publication Date: 2011-12-28
CHONGQING DAXIN PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Another object of the present invention is to provide a new method for extracting intracellular glycopeptides from Versicolor versicolor, so as to overcome the low yield of intracellular glycopeptides of Versicolor versicolor in the prior art, thereby increasing the polysaccharide content

Method used

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  • A method for producing high-purity intracellular glycopeptides of versicolor versicolor by using modern fermentation technology
  • A method for producing high-purity intracellular glycopeptides of versicolor versicolor by using modern fermentation technology
  • A method for producing high-purity intracellular glycopeptides of versicolor versicolor by using modern fermentation technology

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The seed solution was prepared from the slant culture of the strain CVDX-1 through two-layer liquid aerated fermentation culture.

[0037]Incline medium (g / L): glucose 20, yeast extract 5, agar 15, prepared with drinking water, pH natural. Sterilize at 119°C for 30 minutes, connect the preserved strain CVDX- to the culture medium, and cultivate it in a 25°C incubator for 7 days.

[0038] Mother bottle medium (g / L): Glucose 20, yeast powder 10, licicine 2, magnesium sulfate 5, zinc sulfate 5, prepared with drinking water, PH natural. Sterilize at 119°C for 30 minutes, and culture on a reciprocating shaker (60 times / min) in a culture room for 6 days after inoculation, at a culture temperature of 25°C.

[0039] Small tank medium (g / L): glucose 15, soybean powder 15, dextrin 100, magnesium sulfate 5, potassium dihydrogen phosphate 5, calcium carbonate 2. Sterilize at 119°C for 30 minutes, 10 liters of fully automatic stainless steel fermentation tank, The feeding amount i...

Embodiment 2

[0044] The seed solution was prepared from the slant culture of the strain CVDX-1 through two-layer liquid aerated fermentation culture.

[0045] Incline medium (g / L): glucose 20, yeast extract 5, agar 15, prepared with drinking water, pH natural. Sterilize at 122°C for 30 minutes, connect the preserved strain CVDX-1 to the culture medium, and cultivate it in a 28°C incubator for 5 days.

[0046] Mother bottle medium (g / L): Glucose 20, yeast powder 10, licicine 2, magnesium sulfate 5, zinc sulfate 5, prepared with drinking water, PH natural. Sterilize at 123°C for 30 minutes, and culture on a reciprocating shaker (60 times / min) in a culture room for 5 days after inoculation at a culture temperature of 28°C.

[0047] Small tank medium (g / L): glucose 15, soybean powder 15, dextrin 100, magnesium sulfate 5, potassium dihydrogen phosphate 5, calcium carbonate 2. Sterilize at 122°C for 30 minutes, 10 liters of fully automatic stainless steel fermentation tank, The feeding amount ...

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Abstract

By providing a new modern bio-fermentation method for intracellular glycopeptides of Yunzhi, that is, in the production process, continuous feed feeding is adopted and the stirring speed of fermentation is controlled to increase the amount of Yunzhi mycelium, and a new extraction method is adopted at the same time, In order to overcome the problems of low yield of intracellular glycopeptides of Yunzhi in the prior art, etc., the yield and output of intracellular glycopeptides of Yunzhi can be effectively improved. The low-temperature drying process is adopted in the drying step, which overcomes the destruction of the polysaccharide peptide in the intracellular glycopeptide of Versicolor versicolor by the existing high-temperature drying technology, and improves the content of the absorbable polysaccharide peptide, so that it can meet the requirements of the national quality standard and reach the The quality standard of similar products in Japan.

Description

technical field [0001] The invention belongs to the field of medicinal microbial fermentation, and specifically relates to a production method for extracting high-purity intracellular glycopeptides from Yunzhi mycelium by using modern biological fermentation technology. Background technique [0002] Coriolus versicolor is a higher fungus belonging to the family Polyporaceae of the basidiomycete class Polyporaceae. It is a traditional Chinese medicine. It is recorded in "Compendium of Materia Medica" that it has the effects of "strengthening the body, nourishing the essence, nourishing the body, and prolonging life", which is described in modern medicine. It can enhance the immune function of the body and prevent and treat diseases. In 1970, Japanese scholars reported for the first time that a polysaccharide was extracted from the mycelium of Versicolor Versicolor Versicolor (Cariolus Versicolov CM-101), named PS-K, and its functional components were significantly higher than...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K1/14C12R1/645
Inventor 赵德周俊渝
Owner CHONGQING DAXIN PHARMA
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