Method for rapidly determining polysaccharide peptide in lucid ganoderma product

A rapid determination of polysaccharide peptide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome reagent preparation and insufficient stability of test results, and achieve the effect of eliminating impurity interference, simple and fast method, and strong pertinence

Active Publication Date: 2014-06-25
FUJIAN AGRI & FORESTRY UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the only detection method for polysaccharide peptides is the FoLin-phenol method reported by Chen Ying et al. (Chen Ying, Wang Saizhen, Lin Shuqian. Determination of polysaccharide peptide content in Ganoderma lucidum products by FoLin-phenol method[J]. Strait Pharmacy, 2007, 19 (7): 37-39.), the specific operation is to add 1.0 mL of alkaline copper test solution to 1 mL of sample, place it at room temperature for 10 minutes, add 4 mL of FoLin-phenol test solution, bathe in 55 ° C for 5 minutes, cool to room temperature, and measure at 750 nm Absorbance value, but the reagent preparation of this method is cumbersome, and the detection result is not stable enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly determining polysaccharide peptide in lucid ganoderma product
  • Method for rapidly determining polysaccharide peptide in lucid ganoderma product
  • Method for rapidly determining polysaccharide peptide in lucid ganoderma product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Testing instruments and materials

[0030] 1.1 Instrument

[0031] Waters 2515 high performance liquid chromatography system, Waters 2424 evaporative light scattering detector, Empower chromatography workstation and data processing system.

[0032] 1.2. Materials

[0033] Polysaccharide peptide reference substance (>95%, National Juncao Engineering Technology Research Center); Zhengzhou Handu Ganoderma lucidum samples and Shanghai Anqing Ganoderma lucidum samples are Ganoderma lucidum raw materials, purchased from local pharmacies.

[0034] 2. Detection method

[0035] 2.1 Preparation of sample solution

[0036] Weigh 1-2 g of Ganoderma lucidum sample, add 40 mL of water, extract in a 60 °C water bath for 30 min, cool, and dilute to 50 mL; filter, take 2 mL of the filtrate, add 6 mL of absolute ethanol, and mix well. Centrifuge at 10,000 rpm for 15 min, dissolve the precipitate with mobile phase, and filter through a 0.22 μm organic filter membrane to obtain a sa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for rapidly determining polysaccharide peptide in a lucid ganoderma product. The method takes a high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) as a detection means and takes lucid ganoderma polysaccharide peptide as a comparison product to rapidly detect the content of the polysaccharide peptide in the lucid ganoderma product. The pre-treatment of a sample only needs simple extraction operation and is convenient and rapid; finally, after the sample is subjected to chromatography high-performance separation, the evaporative light-scattering detector is used for carrying out quantitative analysis on the polysaccharide peptide in the product; a determination result is high in accuracy and good in stability; the method is better than a current common spectrophotometric method, so as to provide a very effective analyzing method for quality identification and quality control of the lucid ganoderma product.

Description

technical field [0001] The invention belongs to the field of analysis of traditional Chinese medicinal materials, and in particular relates to a method for rapidly determining polysaccharide peptides in ganoderma lucidum products by using an HPLC-ELSD method. Background technique [0002] Ganoderma lucidum is a fungus of the genus Ganoderma of the basidiomycete polyporaceae family. It is a treasure that nourishes and strengthens the body and strengthens the body. It has a long history of medicinal use. The application of modern science and technology makes the study of Ganoderma lucidum more in-depth, and further proves that Ganoderma lucidum and its active ingredients have a wide range of pharmacological effects, which can prevent chronic bronchitis, neurasthenia, hyperlipidemia, coronary heart disease, hepatitis, leukopenia, tumors and other diseases . Ganoderma lucidum polysaccharide peptide, as one of the active ingredients of Ganoderma lucidum, is a variety of glycopep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 林冬梅黄宏南王赛贞罗虹建林占熺林树钱
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products