The invention relates to a method for preparing an
aphid protein sample applicable to
dielectrophoresis, belonging to the field of
agricultural pest prevention and control technologies. The method comprises the following steps of: adding aphids into
liquid nitrogen,
grinding the aphids and the
liquid nitrogen together into
powder, adding 1.5 ml of cold
acetone, evenly mixing, shaking, and centrifuging to collect a precipitate; putting the dried precipitate into
liquid nitrogen and
quartz sand, then
grinding again, and then, adding a saturated
phenol solution and an SDS (
Sodium Dodecyl Sulfate)
buffer solution of the same volume; adding a
methanol-dissolved 0.1M
ammonium acetate solution of a 5 times volume that of a
phenol phase sucked after centrifuging into the
phenol phase, putting the mixture into a refrigerator of -20 DEG C for precipitating for 2 hours, washing the precipitate by using the
methanol-dissolved 0.1M
ammonium acetate solution firstly, and then washing the precipitate by using 80% cold
acetone so as to obtain an
aphid protein crude extract; and dissolving the
aphid protein crude extract into a hydration /
pyrolysis buffer solution, then centrifuging, and collecting an supernatant, thereby obtaining an aphid protein
dielectrophoresis sample. The method has the advantages that the preparation of the aphid protein sample is simple and fast, the dielectrophoretogram with high quality and good
repeatability can be obtained, and the analysis and identification of proteins through a biological
mass spectrometry are facilitated. The method is widely applied to aphid
proteomics research.