Asiatic corn borer larva plasma protein sample preparation method suitable for dimensional electrophoresis

Abstract

An Asiatic corn borer larva plasma protein sample preparation method suitable for dimensional electrophoresis belongs to the technical field of preventing and controlling injurious insects in farmlands. The Asiatic corn borer larva plasma protein sample preparation method comprises the steps that the body surface of Asiatic corn borer larva is sterilized to collect hemolymph, 10mg fo N-phenylthiourea is added into per 200ul of hemolymph, the hemolymph is centrifuged to take supernatant to be separated and packed, 80ul of 50% polyethylene glycol (PEG) is added into each tube, the final concentration is 40%, the hemolymph is shocked and evenly mixed to be in ice bath for 1 hour, the hemolymph is centrifuged to remove supernatant, four times of volume of cold acetone suspension is added into sediment to be shocked and evenly mixed to be in ice bath for 30 minutes, and crude extract of Asiatic core borer larva plasma proteins are dissolved into hydration / crack buffer fluid to be centrifuged to take supernatant to obtain Asiatic corn borer larva plasma protein dimensional electrophoresis sample fluid. The preparation method can prepare Asiatic core borer larva plasma protein samples quickly and easily, can obtain high-quality dimensional electrophoresis patterns with good repeatability and facilitates follow-up researches on biomass spectrometry analysis and identification proteins. The preparation method can be widely applied to researches on the Asiatic core bore larva plasma proteins and has high practical value.

Description

technical field [0001] The invention belongs to the technical field of farmland pest prevention and control, and in particular relates to a method for preparing a plasma protein sample of corn borer larvae suitable for two-dimensional electrophoresis. Background technique [0002] The Asian corn borer, also known as the borer, belongs to the family Lepidoptera. It is the main insect pest of corn, and also damages crops such as sorghum and millet. The corn borer can damage all parts of the corn plant above the ground, making the damaged part lose its function and reducing the grain yield. Due to corn borer damage in the northern spring corn area, the yield is reduced by about 10% in normal years and 20% to 30% in major years. In Jilin Province alone, more than 500 million kilograms are lost due to corn borer damage every year. [0003] With the implementation and advancement of the Human Genome Project, life science research has entered the post-genome era. Proteomics resea...

AI Technical Summary

Problems solved by technology

There are many methods for preparing insect protein, but there is no report on the preparation method for the plasma sample of Ostrinia larvae (especially the plasma protein sample of Ostrinia larvae suitable for two-dimensional electrophoresis analysis)

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