Soft-shelled turtle fatty acid analysis method

Abstract

The invention relates to the field of aquatic organisms and analysis chemistry, in particular to a method for separating and authenticating the components of fatty acid in the body of a soft-shelled turtle by combining GC-MS (gas chromatography-mass spectrometry) with a retention index. Fatty acid methyl esters are prepared by separating the adipose tissue of the soft-shelled turtle, and extracting and deriving the fatty acid from the separated adipose tissue. The method for separating and authenticating the fatty acid comprises the following steps: dissolving fatty acid methyl esters of the soft-shelled turtle in hexane and analyzing by using GC-MS to fulfill the aim of separating; calculating the retention index of fatty acid methyl esters according to the retention time of different fatty acid methyl esters; searching a mass spectrometry library, and meanwhile, carrying out nature determination by combining the retention index, and calculating the relative content of each fatty acid methyl ester by an area normalization method so as to fulfill the aim of analyzing the components of fatty acid of the soft-shell turtle accurately and quickly. The method has the advantages that the components of fatty acid in the soft-shell turtle can be authenticated accurately by fully utilizing the method of searching the mass spectrometry library and nature determination by combining the retention index; particularly, the operation is simple and convenient, and the method has the advantage that the result is accurate compared with the method of searching the mass spectrometry library.

Description

[0001] Technical field: This invention clearly belongs to the field of analytical chemistry, and in particular relates to a method for analyzing fatty acids of soft-shelled soft-shelled turtles by gas chromatography mass spectrometry combined with retention index method. Background technique: [0002] Chinese soft-shelled turtle belongs to Reptiles, Turtles, Turtles, and Soft-shelled Turtles. It is widely distributed in my country except Xinjiang, Tibet, and Qinghai. It is a rare aquatic reptile in my country. Soft-shelled soft-shelled turtle meat is rich in nutrition and high in protein, and is favored by consumers at home and abroad. In addition to being nutritious and delicious, Chinese soft-shelled turtle also has high medicinal value. According to the records in "Bielu of Famous Doctors", soft-shelled turtles are sweet and flat in nature and have the effects of nourishing yin and tonifying kidneys and clearing away deficiency and heat. Turtle shell and turtle blood also h...

AI Technical Summary

Problems solved by technology

Due to the large variety of fatty acids, there are mainly three types of fatty acids in terms of structure: straight-chain fatty acids, branched-chain fatty acids, and complex fatty acids. Different chain lengths, double bond positions and numbers, and substituent groups of fatty acids will lead to inaccurate separation. , especially the complex structure of long-chain fatty acids often has isomers, and is affected by factors such as resolution and chromatographic conditions, which will cause deviations in the mass spectrum retrieval results of fatty acids, making it difficult to accurately characterize, and even appear mistake

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