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Method for identifying lucid ganoderma alkaloids substance finger-print

A technology of alkaloids and fingerprints, which is applied in the field of construction of high-performance liquid chromatography fingerprints, can solve the problems of reporting, identification of alkaloids and quality evaluation without establishing a scientific evaluation method, and achieve peak alkaloids Large number, easy operation of separation effect, and highly targeted effect

Active Publication Date: 2012-05-23
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no scientific evaluation method for the identification and quality evaluation of alkaloids, the biologically active components of different Ganoderma lucidum species, different regions of the same variety, and different cultivation methods for the same variety, and there is no related method report

Method used

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  • Method for identifying lucid ganoderma alkaloids substance finger-print
  • Method for identifying lucid ganoderma alkaloids substance finger-print
  • Method for identifying lucid ganoderma alkaloids substance finger-print

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 establishes the fingerprint of alkaloids in Ganoderma lucidum

[0031] 1. Crushing step: Slice, dry, and pulverize the red Ganoderma lucidum sample of No. 1 variety to about 40 meshes to obtain a dried Ganoderma lucidum.

[0032] 2. Extraction step: Weigh 5g of Ganoderma lucidum dry product, add 95wt% ethanol at a material-to-liquid ratio of 1:33, use 800W power under microwave reflux to extract for 17min, and filter to remove the filter residue to obtain the extract.

[0033] 3. The extract was distilled under reduced pressure to remove the solvent to obtain the alkaloid extract, add methanol to make the volume to 100mL, and obtain the sample solution. Membrane filtration, obtain need testing solution;

[0034] 4. The test solution is analyzed by high performance liquid chromatography to obtain a high performance liquid chromatography diagram. The chromatographic conditions are: Waters1525 high-performance liquid chromatography, binary pump, ultraviolet ...

Embodiment 2

[0036] Example 2 Preliminary Qualitative and Quantitative Comparison, Identification and Analysis of Red Ganoderma Lucidum Alkaloids in Different Production Areas

[0037] Collect 9 kinds of ganoderma lucidum samples from different production areas for alkaloid analysis, the experimental conditions and operation methods are the same as in Example 1, and obtain the alkaloid analysis spectra of 9 kinds of ganoderma lucidum samples, and then use the traditional Chinese medicine chromatographic fingerprint similarity evaluation system to select a The red Ganoderma lucidum sample from the production area is used as the reference peak (R), and the method of multi-point calibration of the chromatographic peak is used to match the chromatographic peaks of the 8 samples and automatically generate the reference spectrum, see the attached figure 2 , through the matching and peak area calculation of the traditional Chinese medicine chromatographic fingerprint similarity evaluation system,...

Embodiment 3

[0047] Example 3 Qualitative analysis of alkaloids using different species of Ganoderma lucidum samples

[0048] Collect 5 different species of red lucidum, Hanzhi, purple lucidum, black lucidum, and wild ganoderma for alkaloid analysis. The sampling volume, experimental conditions and operation methods are the same as in Example 1, and the alkaloid analysis spectra of five ganoderma samples are obtained. Then, using the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints, the red Ganoderma lucidum sample was selected as the reference peak, and the method of multi-point correction of the chromatographic peak was carried out to match the chromatographic peaks of the five samples and automatically generate the reference spectrum, see the attached image 3 According to the matching and peak area calculation of the Chinese medicine chromatographic fingerprint similarity evaluation system, wild Ganoderma lucidum, black lucidum, and Hanzhi all ha...

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Abstract

The invention discloses a method for identifying lucid ganoderma alkaloids substance finger-print, comprising the following steps: adopting 5-10 batches of dried lucid ganoderma fruit body fragments from known standard variety samples produced in different places; taking the same mass of crude medicine in each batch for producing test article solution; and carrying out high performance liquid chromatography analysis on the test article solution; taking common absorption peaks of the high performance liquid chromatography as characteristic chromatographic peaks; choosing chromatographic peaks with uniformization peak area exceeding 1% as fingerprint peaks; obtaining standard lucid ganoderma alkaloids substance finger-print; taking the sample to be tested: dried lucid ganoderma fruit body fragments, obtaining high performance liquid chromatography of the sample to be tested: the lucid ganoderma alkaloids substance by the same method. The high performance liquid chromatography of the sample to be tested: the lucid ganoderma alkaloids substance has characteristic peaks found in the standard finger-print of the lucid ganoderma alkaloids substance, the sample to be tested is a standard variety. With the method of the invention adopted, different lucid ganoderma varieties can be identified.

Description

technical field [0001] The invention belongs to the technical field of quality analysis and identification of alkaloids of raw materials of edible fungi and traditional Chinese medicinal materials, and in particular relates to a construction method and an analysis and identification method of high-performance liquid chromatography fingerprints of alkaloids of biologically active components in Ganoderma lucidum. Background technique [0002] Ganoderma lucidum (Ganoderma Lucidum) has been hailed as a panacea for human beings to prolong life since ancient times, and a fairy grass for nourishing and replenishing qi. As early as in my country's first monograph on herbal science, "Shen Nong's Materia Medica", it is believed that it has the effects of nourishing the heart, calming the soul, nourishing the liver and Qi, and strengthening the bones. Li Shizhen also spoke highly of Ganoderma lucidum's dietary and health care effects in "Compendium of Materia Medica": "Red Zhizhi, also...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/36G01N30/86A61K36/074A61P1/16A61P3/10A61P9/12A61P31/18A61P35/00A61P37/08
Inventor 何晋浙邵平孙培龙蒉霄芸
Owner ZHEJIANG UNIV OF TECH
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