Method for analyzing three tetracycline antibiotics in water environment by HLB solid phase extraction-high performance liquid chromatography

Abstract

The invention relates to a method for analyzing three tetracycline antibiotics in a water environment by HLB solid-phase extraction-high performance liquid chromatography. The method is characterizedby comprising the following specific steps: 1) dissolving an oxytetracycline standard substance, a tetracycline standard substance and an aureomycin standard substance with methanol to prepare a mixedstandard solution with the concentration of 1.00 mu g / ml, gradually diluting the mixed standard solution into solutions with the concentrations of 0.05 mu g / ml, 0.10 mu g / ml, 0.40 mu g / ml, 0.60 mu g / ml and 1.00 mu g / ml, and drawing working curves of three tetracycline antibiotics; 2) performing solid-phase extraction on the three tetracycline antibiotics in the water environment by using an HLB solid-phase extraction column, and concentrating and quantifying the extracted solution by using a parallel quantitative concentrator; and (3) detecting and analyzing the three tetracycline antibioticsconcentrated and quantified in the step (2) by using a high performance liquid chromatography tandem ultraviolet detector. Compared with the traditional method, the HLB solid phase extraction small column has better adsorption capacity to the tetracycline antibiotics, and can meet the requirements of rapid and efficient detection.

Description

technical field [0001] The invention relates to a detection and analysis method for three tetracycline antibiotics in the field of antibiotic detection, in particular to an HLB solid-phase extraction-high performance liquid chromatography method for analyzing three tetracycline antibiotics in a water environment. Background technique [0002] The present invention relates to three tetracycline antibiotics: oxytetracycline, tetracycline and aureomycin. Tetracycline antibiotics are a class of broad-spectrum antibiotics produced by actinomycetes and are widely used in medical treatment, aquaculture and other fields. Due to the large amount of use, it is not easy to degrade in the environment, and has a long-term impact on environmental biology and ecology, such as: affecting the drug resistance of pathogenic bacteria, forming persistent environmental pollution, etc. [0003] At present, there is no unified standard analysis method for the above substances in the domestic water...

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Problems solved by technology

[0003] At present, there is no unified standard analysis method for the above substances in the domestic water environment monitoring field. This paper aims to propose a detection method with efficient pretreatment and accurate analysis.

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