Mixed Bed Ion Exchange Adsorber

Inactive Publication Date: 2017-10-19
MILLIPORE CORP
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0049]Furthermore, the inventive process for purifying a sample comprising a biomolecule of interest and impurities may also be carried out by contacting a sample, with a first chromatography media comprising fibers, said first fibers having imparted thereon functionality enabling ion-exchange chromatography and a second chromatography media comprising fibers, said second fibers having imparted thereon functionality enabling hydrophobic interaction chromatography. In this embodiment of the inventive process, preferably the first chromatography media are cation-exchange fibers and said second chromatography media are hydrophobic interaction chromatography fibers. Otherwise, if the biomolecule of interest it requires said first chromatography media are anion-exchange fibers and said second chromatography media are hydrophobic interaction chromatography fibers. Good purification results are achieved if said first

Problems solved by technology

However, it is often less effective in removing positively charged impurities, such as basic HCP, product aggregates and fragments.
The process of screening hybridoma libraries for candidate mABs is both time consuming and labor intensive.
However, the higher cost of these sophisticated ligands on resins precludes their employment for single use or in disposable processes.
Consequently, the separation process is inherently slow since the rate of mass transport is typically controlled by pore diffusion.
However, the use of small diameter beads comes at the price of increased column pressure drop.
Chromatography media typically has a very high cost (>$1000/L) and significant quantities are required for large scale production columns.
As

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mixed Bed Ion Exchange Adsorber
  • Mixed Bed Ion Exchange Adsorber
  • Mixed Bed Ion Exchange Adsorber

Examples

Experimental program
Comparison scheme
Effect test

example 1

Graft Polymerization of Un-Modified Nylon Fibers

[0207]10 g Allasso nylon fibers and water (466 ml) are added into a 500 ml bottle. 14 ml 1M HNO3 (14.4 mmol) are added to the reaction mixture, followed by the addition of 1.2 ml of a 0.4 M ammonium cerium(IV) nitrate solution in 1M HNO3 (0,480 mmol). The reaction mixture is agitated for 15 minutes. 3.39 g Glycidyl methacrylate (GMA, 24 mmol) are added. Now the agitated reaction mixture is heated to 35° C. for 1 hour. After cooling down to room temperature, the solids are washed with DI water (3×300 ml) and the damp material is used immediately in the following step.

example 2

Q-Functionalization of Epoxy-Functionalized Fibers (AEX Fiber Media)

[0208]The damp GMA functionalized fibers from example 1 are added into a 2 L bottle together with water (500 ml) and a solution of 50 wt % trimethylamine (aq.) in methanol (500 ml). The mixture is agitated for 18 hours at room temperature. Then the fiber solids are subsequently washed with a solution of 0.2 M ascorbic acid in 0.5 M sulphuric acid (3×400 ml), DI water (3×400 ml), 1M sodium hydroxide solution (3×400 ml), DI water (3×400 ml) and ethanol (1×400 ml). Subsequently, the material is placed in an oven to dry at 40° C. for 48 hours.

Yield: 11.74 g of a white fibrous solid

example 3

Graft Polymerization of Un-Modified Nylon Fibers (CEX Fiber Media)

[0209]10 g Allasso nylon fibers and water (460 ml) are added into a 1000 ml bottle. 29.8 ml 1M HNO3 solution (30 mmol) are added to the reaction mixture, followed by the addition of a solution 7.46 ml of a 0.4 M ammonium cerium(IV) nitrate solution in 1M HNO3 (3.00 mmol). The reaction mixture is agitated for 15 minutes. Then 61.5 g 3-sulfopropylmethacrylate potassium salt (3-SPMA, 250 mmol) are added and the resulting agitated reaction mixture is heated to 35° C. for 18 hours. After cooling to room temperature, the fiber solids from each bottle are washed with DI water (3×300 ml), 0.2 M ascorbic acid in 0.5 M sulphuric acid (3×300 ml), DI water (3×300 ml), 1M sodium hydroxide solution (3×300 ml), DI water 3×300 ml) and ethanol (1×300 ml). The prepared material is then placed in an oven to dry at 40° C.

Yield: 11.38 g of a white fibrous solid

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Hydrophobicityaaaaaaaaaa
Interactionaaaaaaaaaa
Login to view more

Abstract

The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and/or hydrophobic interaction.

Description

[0001]The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and / or hydrophobic interaction.BACKGROUNDPurification of Monoclonal Antibodies[0002]Since monoclonal antibodies (mAbs) are used for pharmaceutical applications, they are required in exceptionally high purities [A. Jungbauer, G. Carta, in: Protein Chromatography, Process Development and Scale-Up; WILEY-VCH Verlag, Weinheim (Germany) 2010].[0003]In general mammalian cell cultures are employed to manufacture the majority of therapeutic monoclona...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/20B01D63/02B01D15/38C07K1/36C07K1/18
CPCC07K1/20C07K1/18B01D2311/2626B01D63/02B01D15/3804C07K1/36
Inventor STONE, MATTHEW T.AMARA, JOHN P.
Owner MILLIPORE CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap