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Separation purification method for natural abscisic acid

A technology for the separation and purification of natural abscisic acid, applied in the field of biofermentation-biochemical pesticide production, can solve the problems of low purification efficiency and high cost of natural abscisic acid, and achieve the effect of less product residue, simple method and high purity

Inactive Publication Date: 2016-03-30
江西新瑞丰生化股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiency of low purification efficiency and high cost of natural abscisic acid at present, and provides a separation and purification method of natural abscisic acid with high yield and low cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The abscisic acid fermentation liquid is filtered through the plate and frame to obtain the plate and frame filtrate, the pH is adjusted to 4.9, and the ultrafiltration membrane with a molecular weight cut off of 3000 is used to treat it to obtain an ultrafiltration dialysate, which is concentrated through a nanofiltration membrane with a molecular weight cut off of 100 , to obtain a concentrated solution, the concentrated solution is acidified and then extracted with ethyl ester, the extract is concentrated to 60 mg / ml, then dichloromethane is added, impurities are adsorbed by silica gel column chromatography, the obtained chromatographic solution is concentrated under reduced pressure, and the vacuum degree is -0.084Mpa, jacket circulating water temperature is 60°C, material temperature is 40°C, cooled and crystallized, filtered and dried to obtain abscisic acid product with a content of 96%.

Embodiment 2

[0025] The abscisic acid fermentation broth is filtered through the plate and frame to obtain the plate and frame filtrate, the pH is adjusted to 5.5, and the ultrafiltration membrane with a molecular weight cut off of 5000 is used to obtain the ultrafiltration dialysate, which is concentrated through a nanofiltration membrane with a molecular weight cut off of 150 , to obtain a concentrated solution, the concentrated solution is acidified and extracted with butyl ester, the extract is concentrated to 70mg / ml, then petroleum ether is added, impurities are adsorbed through alumina column chromatography, the obtained chromatographic solution is concentrated under reduced pressure, and the vacuum degree is -0.09Mpa, jacket circulating water temperature is 58°C, material temperature is 38°C, after concentration, add 0.8 times of hexane, cool and crystallize, filter and dry to obtain abscisic acid product with a content of 95.2%.

Embodiment 3

[0027] The abscisic acid fermentation liquid is filtered through the plate and frame to obtain the plate and frame filtrate, the pH is adjusted to 6.0, and the ultrafiltration membrane with a molecular weight cut off of 10000 is used to obtain the ultrafiltration dialysate, which is concentrated through a reverse osmosis membrane with a molecular weight cut off of 50 , to obtain a concentrated solution, the concentrated solution was acidified and extracted with isoamyl acetate, the extract was concentrated to 80 mg / ml, then chloroform was added, and impurities were adsorbed by magnesium silicate column chromatography, the obtained chromatographic solution was concentrated under reduced pressure, vacuum The temperature is -0.092Mpa, the temperature of the jacket circulating water is 45°C, and the material temperature is 30°C. During the concentration process, 0.7 times of chloroform is added, cooled and crystallized, filtered, and dried to obtain an abscisic acid product with a c...

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Abstract

A separation purification method for natural abscisic acid belongs to the field of biological fermentation and production of biochemical pesticides. The method comprises performing plate-frame filtration on an abscisic acid fermentation liquid to obtain a plate-frame filtrate, then processing the filtrate through an ultrafiltration membrane and a condensation membrane, so as to obtain a concentrate, acidifying the condensate, then performing extraction, pretreating the extraction liquid, then adding an eluent, adsorbing impurities through adsorption column chromatography, performing reduced-pressure concentration on the chromatography liquid, adding a non-polar solvent into the concentrate, filtering and drying, so as to obtain the abscisic acid product. The method is simple and convenient, abscisic acid extracted from the fermentation liquid is high in purity and high in yield, and product residue in the mother liquid is less.

Description

technical field [0001] The invention relates to the field of biofermentation-biochemical pesticide production, in particular to a separation and purification method of natural abscisic acid. Background technique [0002] Abscisic acid is an endogenous plant hormone with comprehensive physiological functions. It is one of the five major plant hormones. It is an important growth regulator for plants and a key factor for balancing the metabolism of plant endogenous hormones and related growth active substances. Water, fertilizer and the ability to coordinate metabolism in the body can effectively regulate the root / shoot, vegetative growth and reproductive growth of plants, and play an important role in improving the quality and yield of crops. Abscisic acid is a product with a very promising market prospect. It has broad application prospects in agricultural production and can generate huge economic and social benefits. At present, the price of abscisic acid in the market is s...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/47C07C51/43C07C59/90
CPCC07C51/42C07C51/43C07C51/47
Inventor 周金龙彭海刚雷文赵南
Owner 江西新瑞丰生化股份有限公司
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