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Method for increasing titer of bacitracin in fermentation liquid by using oxygen carrier

A technology of oxygen carrier and bacitracin, applied in the field of microorganisms, can solve the problems of no good effect, decreased strain vitality, large randomness, etc., and achieve the effects of reducing energy consumption, increasing production, and increasing transfer rate

Inactive Publication Date: 2013-01-30
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on the production of bacitracin by microbial fermentation is mainly the optimization of fermentation conditions, such as the optimization of the medium and the optimization of the fermentation mode, which has not achieved good results; some factories have also screened high-yield bacitracin through traditional microbial mutation breeding strains, the disadvantage of this method is that the randomness is too large, and the long-term mutagenesis will make the vigor of the strains continue to decline

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for improving the titer of bacitracin in a fermentation broth by using an oxygen carrier, the steps are as follows:

[0024] (1) First-level seed cultivation: Inoculate Bacillus licheniformis as a production strain into a seed medium with a pH of 7.2 from a slant, and cultivate it on a rotary shaker at 160r / min at a temperature of 37°C for 24 hours to obtain a seed suitable for inoculation. The seed culture medium contains: beef extract 10g / L, peptone 5g / L and sodium chloride 10g / L.

[0025] ⑵Secondary fermentation culture: Inoculate the seed culture solution into the fermentation medium at an inoculation amount of 5% (v / v), and add filter-sterilized 1% (v / v) n-dodecane before inoculation. The fermentation medium contains: corn steep liquor 35g / L, sucrose 12g / L, ammonium sulfate 3g / L, calcium carbonate 2g / L, corn flour 25g / L, pH=7.2.

[0026] (3) The fermentation culture method is carried out on a 7L fermenter with a liquid volume of 3.5L, 250rpm, and 37°C for...

Embodiment 2

[0029] A method for improving the titer of bacitracin in a fermentation broth by using an oxygen carrier, the steps are as follows:

[0030] (1) First-level seed cultivation: Same as (1) in Implementation Case 1.

[0031] ⑵Secondary fermentation culture: Inoculate the seed culture solution into the fermentation medium at an inoculum size of 5% (v / v), and add filter-sterilized 2% (v / v) oleic acid before inoculation. The fermentation medium contains: corn steep liquor 30g / L, sucrose 15g / L, ammonium sulfate 2g / L, calcium carbonate 3g / L, corn flour 20g / L, pH=7.2.

[0032] (3) The fermentation culture method is carried out on a 7L fermenter, and the liquid volume of the fermenter is 3.5L. The fermentation was carried out for 46 hours under the conditions of 250 rpm and 37° C. temperature.

[0033] In this example, the titer of bacitracin in the fermentation broth was 562.7u / ml, which was 7.71% higher than the titer of 522.4u / ml in the medium without oxygen carrier.

Embodiment 3

[0035] A method for improving the titer of bacitracin in a fermentation broth by using an oxygen carrier, the steps are as follows:

[0036] (1) First-level seed cultivation: Same as (1) in Implementation Case 1.

[0037] (2) Fermentation culture: Inoculate the seed culture liquid into the fermentation medium at an inoculation amount of 5% (v / v), and add filter-sterilized 5% (v / v) soybean oil before inoculation. The fermentation medium contains: corn steep liquor 30g / L, sucrose 13g / L, ammonium sulfate 4g / L, calcium carbonate 2g / L, corn flour 25g / L, pH=7.2.

[0038] (3) The fermentation culture method is carried out on a 7L fermenter, and the liquid volume of the fermenter is 3.5L. The fermentation was carried out for 42 hours under the conditions of 200 rpm and 37° C. temperature.

[0039] In this example, the titer of bacitracin in the fermentation broth was 547.1u / ml, which was 4.73% higher than the titer of 522.4u / ml in the culture medium without oxygen carrier.

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Abstract

The invention relates to a method for increasing titer of bacitracin in fermentation liquid by using oxygen carrier. A certain amount of oxygen carrier is added into a fermentation medium to improve the oxygen dissolution condition and greatly increase titer of bacitracin fermentation liquid, without changing other fermentation conditions. The method has simple process; the oxygen carrier in the production process can be recycled, so as to reduce production cost; therefore, the method has an important guiding significance for antibiotic production.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to an optimized application technology in a microorganism fermentation process, in particular to a method for increasing the titer of bacitracin in a fermentation liquid by using an oxygen carrier. Background technique [0002] Bacitracin, white or off-white powder in appearance, odorless or slightly smelly, bitter in taste, hygroscopic, soluble in water, methanol and ethanol, insoluble in acetone, ether and chlorine. Bacitracin is a broad-spectrum antibiotic that has a strong inhibitory effect on Gram-positive bacteria, especially pathogenic bacteria such as Clostridium, Staphylococcus, Streptococcus, Corynebacterium, and Neisseria. It is sensitive and has a strong inhibitory effect on penicillin-resistant Staphylococcus aureus. It is suitable for the treatment of abscesses, acne, purulent skin diseases, amoebic dysentery, gonorrhea and meningococcus and other infectious diseases, and i...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/10
Inventor 别松涛李建勋路福平谢进霞徐星蔡鑫
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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