Tylosin extraction menstruum and extraction method

A technology of tylosin and extraction method, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of high BA price, large dosage, and limited extraction capacity, and achieve small dosage and high extraction efficiency Effect

Active Publication Date: 2012-07-18
宁夏泰瑞制药股份有限公司
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ethyl acetate has a big disadvantage: the solubility in water at room temperature is 8.08% by mass fraction, and it is easy to decompose after long-term storage or use, and the hydrolysis products are acetic acid and ethanol
However, the disadvantages of extracting tylosin from the fermentation broth with BA are as follows: first: the price of BA is high and the dosage is large
Second: the extraction capacity of BA is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Extracting Tylosin from Fermentation Filtrate with Butyl Acetate

[0020] Secondary countercurrent extraction: Tylosin fermentation filtrate 60m 3 , The titer is 13523u / ml. Organic solvent butyl acetate 18m 3 . Turn on the centrifuge, and after the speed and current are normal, open the valves of each material pipeline, and at the same time open the steam valve of the pipeline heater to heat the material, so that the temperature of the fermentation liquid entering the centrifuge is at 44°C. During the extraction process, samples were taken to check the separation between the composite solvent phase and the water phase, the potency and the pH value of the water phase. The primary pH of the extract was 7.0-10.0, and the secondary pH was 8.0-11.0. After the extraction, the volume of the aqueous phase is 58m 3 , The titer is 748u / ml. The volume of butyl acetate is 17.1m 3 , the titer is 41898u / ml. It took 4.1h. The extraction yield was 88.3%.

[0021] Bac...

Embodiment 2

[0023] Example 2 Extracting Tylosin from Fermentation Filtrate with Composite Solvent

[0024] Secondary countercurrent extraction: Tylosin fermentation filtrate 60m 3 , The titer is 13416u / ml. Composite solvent 15m 3 (Butyl acetate 9.1 m 3 , Isobutyl acetate 4.65m 3 , butanol 1.2m 3 ). Turn on the centrifuge, and after the speed and current are normal, open the valves of each material pipeline, and at the same time open the steam valve of the pipeline heater to heat the material, so that the temperature of the fermentation liquid entering the centrifuge is at 40°C. During the extraction process, samples were taken to check the separation between the composite solvent phase and the water phase, the potency and the pH value of the water phase. The primary pH of the extract was 7.0-10.0, and the secondary pH was 8.0-11.0. After the extraction, the volume of the aqueous phase is 57m 3 , The titer is 286u / ml. Composite solvent volume is 14.6m 3 , the titer is 52542u / ml. ...

Embodiment 3

[0027] Example 3 Extracting Tylosin from Fermentation Filtrate with Composite Solvent

[0028] Tylosin Ferment Filtrate 60m 3, The titer is 13462u / ml. Emulsification occurred in the fermented liquid, and 204 L of demulsifier sodium dodecylbenzenesulfonate was added. Add composite solvent 15.6m 3 (Butyl acetate 9.4 m 3 , Isobutyl acetate 4.8m 3 , butanol 1.4m 3 ). Turn on the centrifuge, and after the speed and current are normal, open the valves of each material pipeline, and at the same time open the steam valve of the pipeline heater to heat the material, so that the temperature of the fermentation liquid entering the centrifuge is at 45°C. During the extraction process, samples were taken to check the separation between the composite solvent phase and the water phase, the potency and the pH value of the water phase. The primary pH of the extract was 7.0-10.0, and the secondary pH was 8.0-11.0. After the extraction, the volume of the aqueous phase is 57m 3 , The tite...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a compound menstruum for extracting tylosin and an extraction method. According to the invention, the compound menstruum formed by mixing butyl acetate, isobutyl acetate and butanol according to the volume ratio of 6:3 to 3.2 to 0.8-1 is used for extracting tylosin in fermentation filtrate through a secondary counter-current extraction method. The method has the advantages of low consumption of organic menstruum, low loss of tylosin, short extraction time, high extraction yield and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine extraction, and in particular relates to a tylosin extraction solvent and an extraction method. Background technique [0002] Tylosin, also known as tylosin, is a macrolide antibiotic obtained by fermenting and extracting actinomycetes Streptomyces freundii. There are three types of tylosin base, phosphate and tartrate form. Tylosin is a high-efficiency, non-residue antibacterial and growth-promoting agent for poultry and livestock. It has a unique curative effect on chicken septicemia, porcine epidemic pneumonia and other diseases, and has obvious promoting effect on the growth of poultry and livestock. As an animal-specific antibiotic, tylosin avoids the problem of cross-resistance caused by shared antibiotics between humans and animals, and is used for the prevention and treatment of swine and poultry mycoplasma diseases. [0003] At present, the domestic tylosin extraction process is ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06
Inventor 任勇冷晓红王友善奇乃董媛李小萍
Owner 宁夏泰瑞制药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap