Thallus extract and preparation method thereof

An extract and cell technology, which can be applied to the environmental protection treatment and comprehensive utilization of Tyler bacteria residue and vitamin B12 wastewater, the treatment and comprehensive utilization of wastewater, and the field of antibiotic fermentation industrial waste residue, which can solve the problem of destroying soil micro-ecological balance and waste of resources. , human hazards and other problems, to achieve the effect of solving pollution and utilization problems, solving difficult drying, and reducing overall costs

Inactive Publication Date: 2009-09-09
迈德金生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, some manufacturers dried it and used it as feed protein. However, because there is a certain amount of tylosin residue in it, referred to as "drug residue", it will form "anti-meat" and "anti-egg" after it is ingested into animals as feed protein. products that pose a direct hazard to humans
Of course, it is also used as agricultural fertilizer after drying, but if the drug residue is not resolved, it will directly d

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture Embodiment 1

[0020] Manufacturing Example 1: Treating 1000 kg of Tylos slag to prepare the bacterium extract:

[0021] 1. Strain preparation: the strains were Saccharomyes cerevisiae Meyenex Hansen CGMCC AS 2.121, Lactobacillus fermentum AS 1.122, Bacillus cereus CGMCC AS 1.895, and three strains were cultured in combination;

[0022] Preparation of seed liquid: The culture medium is: 50 grams of brown sugar, 15 grams of peptone, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 10 grams of ammonium sulfate, 0.3 grams of calcium carbonate, and 5 grams of sodium chloride. To 1000ml, sterilize at 115°C for 20 minutes; after cooling down, insert Saccharomyces cerevisiae, Lactobacillus fermentum, and Bacillus cereus that have been activated more than twice, control the temperature at 34±1°C with intermittent aeration and stirring, and cultivate for 36 hours. Obtain the primary compound seed liquid; prepare 100 L of secondary compound seed liquid in the same way.

[002...

manufacture Embodiment 2

[0025] Manufacturing Example 2: Treating 1000 kg of Tylos scum to prepare powdery thallus extract

[0026] 1. Strain preparation: the strains were Saccharomyes cerevisiae Meyenex Hansen CGMCC AS 2.610, Lactobacillus delbrueckii subsp bulgaricus CGMCC AS 1.1482, and Bacillus subtilis CGMCC AS 1.140;

[0027] (1), the cultivation of lactic acid bacteria:

[0028] First-class seeds: Culture medium: 10 grams of glucose, 5 grams of yeast extract, 1 gram of sodium chloride, 0.5 grams of dipotassium hydrogen phosphate, 0.1 grams of magnesium sulfate, 1 gram of ammonium sulfate, 0.1 grams of calcium carbonate, and dilute to 1000 with water ml, sterilized at 115°C for 10 minutes; insert activated Lactobacillus delbrueckii after cooling down, and culture at a controlled temperature of 36±1°C for 48 hours;

[0029] Secondary seeds: The culture medium is: 1000 grams of glucose, 250 grams of yeast extract, 100 grams of sodium chloride, 10 grams of dipotassium hydrogen phosphate, 10 grams ...

manufacture Embodiment 3

[0038] Manufacturing Example 3: Treating 1000 kg of Tylos scum to prepare powdery thallus extract

[0039] 1. Strain preparation: Saccharomyes cerevisiae Meyenex Hansen CGMCC AS 2.121 and Bacillus licheniformis CGMCC AS 1.932 were selected as yeasts;

[0040] Preparation of primary seeds: the culture medium is: 50 grams of molasses, 5 grams of beef extract, 2.5 grams of sodium chloride, 5 grams of dipotassium hydrogen phosphate, 2 grams of magnesium sulfate, 10 grams of ammonium sulfate, and 0.5 grams of calcium carbonate. Dilute to 1000 ml, sterilize at 100°C for 10 minutes; insert activated Saccharomyces cerevisiae and Bacillus after cooling down, and culture at a controlled temperature of 30±1°C for 72 hours to obtain a first-grade compound seed liquid;

[0041] Preparation of secondary seeds: the culture medium is: 10 kg of molasses, 1 kg of beef extract, 500 grams of sodium chloride, 1 kg of dipotassium hydrogen phosphate, 400 grams of magnesium sulfate, 2 kg of ammonium ...

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PUM

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Abstract

The invention relates to the field of comprehensive utilization of tylosin residues and waste water of vitamin B12, and provides a method which is to use the tylosin residues and the waste water of the vitamin B12 as raw materials, separate and extract protoplasm substances in a mycelium to prepare a thallus extract through microbial fermentation treatment, and apply the thallus extract in a biological medium. The thallus extract and the preparation method thereof open a new path for utilization of the tylosin residues and the waste water of the vitamin B12, and provide possibility of realizing clean production which is a goal of sustainable development for the fermentation industry of the tylosin and the vitamin B12. Furthermore, the method solves the problem that the tylosin residues and the waste water of the vitamin B12 pollute environment and are harmful to human health.

Description

Technical field: [0001] The invention relates to the technical field of treatment and comprehensive utilization of antibiotic fermentation industrial waste residue and waste water, in particular to the field of environmental protection treatment and comprehensive utilization of Tylos slag and vitamin B12 waste water. Specifically, it provides a bacterium extract prepared from Tylos slag and vitamin B12 waste water as raw materials after microbial fermentation treatment; the bacterium extract can be used as a nitrogen source in a biological medium and growth Factors, used in bio-fermentation industry. Background technique: [0002] Tylosin is a metabolite of liquid submerged fermentation of Streptomyces and other producing bacteria. During the production process of liquid submerged fermentation, the producing bacteria not only synthesize the desired product tylosin, but also produce a large amount of mycelium and fermentation broth. After treatment and solid-liquid separatio...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P1/04C12R1/645C12R1/225C12R1/07
Inventor 马兰孙玉胜冯艳霞
Owner 迈德金生物技术(北京)有限公司
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