Tylosin degrading bacteria and application thereof

A technology for tylosin and degrading bacteria, which is applied in the fields of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., and can solve problems such as environmental residues of tylosin

Active Publication Date: 2019-04-19
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: how to provide a fast and efficient strain for degrading tylosin, so as to solve the problem of tylosin residues in the environment

Method used

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  • Tylosin degrading bacteria and application thereof
  • Tylosin degrading bacteria and application thereof
  • Tylosin degrading bacteria and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Isolation and Screening of Tylosin Degrading Bacteria

[0020] On November 20, 2017, a sample of Tylos dregs was obtained from Zhongmu Biopharmaceutical Co., Ltd., Tuoketuo County, Hohhot City, Inner Mongolia Autonomous Region. Weighed 10g of the sample and put it in a triangular flask, and added it to 90ml of phosphate buffer. Place in a shaker at 30°C and 150rpm for 2 hours, take it out and let it stand. Dilute the supernatant to 10 -5 、10 -6 、10 -7 , absorb 100 μm supernatant and apply to beef extract peptone solid medium (peptone 5g, NaCl 5g, beef extract 3g, agar 18g, distilled water to 1000mL, use 1mol / L sodium hydroxide to adjust the pH to 7.0~7.2 and then kill Bacteria.) and cultured at 30°C for 3 days. According to the colony growth rate, size, shape, color and other characteristics, the colonies were picked and streaked on the beef extract peptone solid medium plate, and repeated several times until a single bacterium was isolated. Store in a 4°C refriger...

Embodiment 2

[0042] Optimal determination of TYL2 degradation conditions

[0043] The optimal culture conditions for Tylosin removal by Tylosin degrading bacteria were determined by single factor test. The measured content includes temperature (25°C-45°C), rotating speed (90r / min-200r / min), pH (5-11), tylosin concentration (25mg / L-150mg / L), inoculum size (1 %-10%), the culture time is 7 days. Three parallels were set for each of the above treatments. See the test results figure 2 . from figure 2 It can be seen that TYL2 is at 35°C, 150r / min, pH=7, and the inoculum size is 1.4×10 9 CFU / mL, when the concentration of tylosin is 25mg / L, after 7 days of treatment under optimized conditions, the degradation rate reaches the highest of about 75%.

Embodiment 3

[0045] Determination of Degradation Efficiency of Tylosin Degrading Strain TYL2 in Sewage

[0046] The screened highly efficient degrading bacteria TYL2 was added to the sewage samples, cultured for 7 days under the optimal conditions of the degrading bacteria TYL2, and the residual amount of tylosin was detected. See the test results image 3 . from image 3 It can be seen that TYL2 can degrade more than 60% of tylosin in sewage under optimized conditions. It shows that the bacteria can be used for the degradation of tylosin. The strain TYL2 and the bacterial agent produced can be directly applied to the treatment of tylosin enterprise waste, and can also be applied to the restoration of the surrounding soil and water environment polluted by tylosin.

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Abstract

The invention discloses tylosin degrading bacteria and application thereof, and belongs to the technical field of microorganism degrading antibiotics residues. The tylosin degrading bacteria TYL2 is brevibacillus borstelensis TYL2 and is preserved in the common microorganism center of the China microorganism culture preservation management council, wherein the preservation date is 29 / 12 / 2018, thepreservation serial number is CGMCC No.17051, and the preservation address is No.3 microorganism research laboratory of Chinese Academy of Sciences in No.1 yard at the Beichen west road of Chaoyang district of Beijing. With drug residues being primary raw materials for sieving, an obtained strain and a prepared fungicide can be directly used for treating waste of a tylosin enterprise, and can be also used for repairing surrounding soil and a water body environment which are polluted by tylosin.

Description

technical field [0001] The invention belongs to the field of microorganism degrading antibiotics, and in particular relates to a tylosin degrading bacterium and application thereof. Background technique [0002] Tylosin, also known as Tylosin or Tynon, was obtained by American scientists in 1959 in the study of a strain called Streptomycesfradiae (Streptomycesfradiae). Macrolide antibiotics. Tylosin is a white plate-like crystal, slightly soluble in water and alkaline. The molecular formula of Tylosin is: C 46 h 77 NO 17 , relative molecular mass: 916.1, chemical structural formula: [0003] [0004] Tylosin is a broad-spectrum antibiotic with specific effects on mycoplasma and a variety of G + Bacteria have a strong antibacterial effect, but also for some G － Bacteria, Campylobacter, Spirochetes and Coccidia have inhibitory effect. [0005] At present, the environmental residue problem of tylosin has become one of the hot environmental issues that are widely conc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/38C12R1/08
CPCC12N1/20C02F3/34B09C1/10C02F2101/38C12R2001/08C12N1/205
Inventor 李兆君冯长青冯瑶
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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