9 results about "EIF4E" patented technology

Methods and compositions for treatment of prostate cancers, such as androgen receptor (AR) deficient and androgen receptor (AR) low cancers, are disclosed. The methods include administration of an agent that inhibits activity of eIF4F or an agent that disrupts the eIF4F translation-initiation complex (composed of eIF4E, eIF4A, and eIF4G).

It provides a complex for target protein degradation. [Solution] The present invention relates to a novel compound-peptide nucleic acid-aptamer complex for targeted protein degradation and its applications. The complex is manufactured by binding a novel compound to a peptide nucleic acid and introducing an aptamer that can bind complementaryly to the peptide nucleic acid. It has been confirmed to have the effect of targeting and degrading intracellular target proteins such as Tau, nucleolin, and eIF4E, and can be usefully utilized in related industries.

The invention relates to application of a reagent for inhibiting STAT3 gene expression in treatment of oxidative damage of trabecular meshwork cells. According to the invention, a signal channel STAT3 to EIF4E to TGF-beta-SMAD < 2 / 3 > for regulating and controlling the oxidative damage of the trabecular meshwork cells is innovatively found, and the STAT3 activates the TGF-beta-SMAD < 2 / 3 > channel by combining with EIF4E3, so that the apoptosis, oxidative stress and fibrosis of the trabecular meshwork cells caused by the oxidative damage are promoted. After the expression of the STAT3 gene in trabecular meshwork cells is inhibited, the EIF4E3 level can be reduced, and the apoptosis, oxidative stress and fibrosis of the trabecular meshwork cells induced by EIF4E3 are improved. Therefore, a reagent for inhibiting STAT3 gene expression can be used for treating trabecular meshwork cell oxidative damage and eye diseases related to the trabecular meshwork cell oxidative damage, including glaucoma and the like.

The invention relates to the technical field of gene editing, in particular to a tobacco multi-gene editing expression vector and a construction method and application thereof. According to the invention, a CRISPR / Cas9 gene editing system based on a tRNA spacer element is used for simultaneously targeting DHS, POD3, CSE, LPEAT, LHT1 and eIF4E of tobacco, mutant materials for simultaneously editing multiple genes are screened out, new germplasm is provided for creating excellent tobacco varieties, and the invention belongs to the technical field of gene editing.

The invention discloses an EIF4E-SV288 molecular marker for distinguishing a fragrant pig from a large white pig and a detection technology of the molecular marker, and belongs to the field of livestock molecular biology detection. The molecular marker SV288 is located in a pig reference genome Sscrofa 11.1 chr8: 121455580-121455867, the length of the molecular marker SV288 is 288bp, and the molecular marker SV288 is contained in a fourth intron of an EIF4E gene, has two types of a deletion type (allele D) and a non-deletion type (allele I), and corresponds to three genotypes of DD, ID and II. The invention also provides a primer for specifically detecting the molecular marker, a PCR detection method and an application method for distinguishing the fragrant pig from the large white pig. The detection result shows that the fragrant pig only contains the DD genotype, and the frequency of the D allele is 100%; large white pigs are mainly composed of II and ID genotypes, the frequency of D allele is 30.93%, and the difference between II and ID genotypes is very significant (Plt; the molecular marker is not limited by age, gender and feeding conditions of pigs, the detection method is accurate and reliable, the operation is simple and convenient, and variety distinguishing of fragrant pigs and large white pigs can be efficiently realized.

Novel retinamides with unique properties and biological activities, including inhibition of retinoic acid metabolism and modulation of Mnk1 / 2-eIF4E and androgen receptor (AR) signaling pathways, are synthesized and developed. The novel retinamides are capable of effecting treatment of a variety of dermatological conditions while limiting adverse side effects.

The invention discloses a red light reversible regulation gene expression system and application, the system comprises three plasmid vectors, respectively a plasmid pPA formed by fusing a PUF protein and a phytochrome PhyA, a plasmid pFE formed by fusing a shuttle protein FHY1 and eIF4E, and a downstream targeting regulation plasmid pNG formed by an NRE target and a downstream gene of interest GOI; according to the invention, the binding characteristic of RNA binding protein PUF and target NRE thereof is utilized, and the characteristic of dimerization of PhyA protein and ligand FHY1 thereof under red light is fused, so that a gene expression activation system capable of bidirectional light regulation is constructed, the PUF protein continuously and stably inhibits expression of downstream genes of interest, background leakage of the system is reduced from the source, and the expression of the downstream genes of interest is inhibited. Expression of downstream interested genes can be caused only under irradiation of red light, the expression can be rapidly closed by far-red light, and the expression of the genes can be accurately controlled through light; the system can be applied to tumor treatment.