Specific co-dominance molecular marker for large-fragment deletion mutation of eIF4E-1 site of tobacco and application of molecular marker

Abstract

The invention discloses a specific co-dominance molecular marker for large-fragment deletion mutation of an eIF4E-1 site of tobacco and application of the molecular marker. A marker relevant with thedetection of a wild site is named as ASM-W and is represented by SEQ ID No.1, and the fragment size is 763bp; and a marker relevant with the detection of a corresponding base deletion mutation site isnamed as ASM-m and is represented by SEQ ID No.2, and the fragment size is 572bp. The invention further discloses a primer for identifying the specific co-dominance molecular marker for the large-fragment deletion mutation, and the sequences of the primer are represented by SEQ ID NO.3, 4 and 5. By utilizing the markers and the primer, the genotype of backcross transformation descendants of PVY resistance donor parents can be accurately selected. The markers and the primer can be applied to the germplasm resource identification and assist breeding of the tobacco and the selection of germplasmmaterials or transformed descendants in the PVY resistance mutation type.

Description

technical field [0001] The present invention relates to the development of a large fragment mutant of a gene and its related site-specific molecular markers, in particular to a mutation of the eukaryotic translation initiation factor eIF4E-1 in tobacco and the development and development of its site-specific molecular markers. application. The mutant can provide a source of variation for the selection of virus-resistant tobacco germplasm containing the mutation site, and the site-specific molecular markers can be directly applied to molecular marker-assisted breeding to select tobacco materials containing the mutation site and improve eIF4E-1 mutation The invention relates to the selection efficiency of a body, and belongs to the field of biotechnology. Background technique [0002] Tobacco potato virus Y disease, also known as vein spot disease, is a systemic infection disease of tobacco caused by potato virus Y (PVY). The loss caused by this disease varies with the perio...

AI Technical Summary

Problems solved by technology

This marker can distinguish some disease-resistant flue-cured tobacco (or Burley tobacco) varieties from susceptible varieties, but cannot effectively distinguish the homozygous and heterozygous genotypes of wild-type eIF4E-1, for example, the applicant has verified through sequence analysis and PCR , found that the application of CF2 / GR11 primers can amplify approximately specific bands in 6 PVY resistant sun-cured varieties and 8 PVY susceptible flue-cured or sun-cured varieties (such as figure 1 shown), so it cannot effectively distinguish these tobacco PVY-resistant varieties, and this molecular marker cannot be directly used for assisted selection when backcrossing the mutant eIF4E-1 locus

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